Super-Resolution Reconstruction for Two-and Three-Dimensional LA-ICP-MS Bioimaging

Westerhausen, MT; Bishop, DP; Dowd, A; Wanagat, J; Cole, N; Doble, PA

Super-Resolution Reconstruction for Two-and Three-Dimensional LA-ICP-MS Bioimaging

Westerhausen, MT Bishop, DP

Dowd, A

Wanagat, J Cole, N

Doble, PA

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Publication Type:: Journal Article
Citation:: Analytical Chemistry, 2019
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Westerhausen, MT	en_US
dc.contributor.author	Bishop, DP https://orcid.org/0000-0002-6533-4410	en_US
dc.contributor.author	Dowd, A https://orcid.org/0000-0002-0500-3599	en_US
dc.contributor.author	Wanagat, J	en_US
dc.contributor.author	Cole, N https://orcid.org/0000-0002-9866-4694	en_US
dc.contributor.author	Doble, PA https://orcid.org/0000-0002-8472-1301	en_US
dc.date.accessioned	2020-03-17T05:55:22Z
dc.date.available	2020-05-25T19:05:58Z
dc.date.issued	2019-01-01	en_US
dc.identifier.citation	Analytical Chemistry, 2019	en_US
dc.identifier.issn	0003-2700	en_US
dc.identifier.uri	http://hdl.handle.net/10453/139369
dc.description.abstract	© 2019 American Chemical Society. The resolution of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) elemental bioimaging is usually constrained by the diameter of the laser spot size and is often not adequate to explore in situ subcellular distributions of elements and proteins in biological tissue sections. Super-resolution reconstruction is a method typically used for many imaging modalities and combines multiple lower resolution images to create a higher resolution image. Here, we present a super-resolution reconstruction method for LA-ICP-MS imaging by ablating consecutive layers of a biological specimen with offset orthogonal scans, resulting in a 10× improvement in resolution for quantitative measurement of dystrophin in murine muscle fibers. Layer-by-layer image reconstruction was also extended to the third dimension without the requirement of image registration across multiple thin section specimens. Quantitative super-resolution reconstruction, combined with Gaussian filtering and application of the Richardson-Lucy total variation algorithm, provided superior image clarity and fidelity in two-and three-dimensions.	en_US
dc.relation.ispartof	Analytical Chemistry	en_US
dc.relation.isbasedon	10.1021/acs.analchem.9b02380	en_US
dc.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in [Analytical Chemistry, 2019], copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see [insert ACS Articles on Request author-directed link to Published Work, see https://pubs.acs.org/doi/abs/10.1021/acs.analchem.9b02380].”
dc.subject.classification	Analytical Chemistry	en_US
dc.title	Super-Resolution Reconstruction for Two-and Three-Dimensional LA-ICP-MS Bioimaging	en_US
dc.type	Journal Article
utslib.for	0301 Analytical Chemistry	en_US
utslib.for	0399 Other Chemical Sciences	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	open_access
pubs.publication-status	Published	en_US

Abstract:

© 2019 American Chemical Society. The resolution of laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) elemental bioimaging is usually constrained by the diameter of the laser spot size and is often not adequate to explore in situ subcellular distributions of elements and proteins in biological tissue sections. Super-resolution reconstruction is a method typically used for many imaging modalities and combines multiple lower resolution images to create a higher resolution image. Here, we present a super-resolution reconstruction method for LA-ICP-MS imaging by ablating consecutive layers of a biological specimen with offset orthogonal scans, resulting in a 10× improvement in resolution for quantitative measurement of dystrophin in murine muscle fibers. Layer-by-layer image reconstruction was also extended to the third dimension without the requirement of image registration across multiple thin section specimens. Quantitative super-resolution reconstruction, combined with Gaussian filtering and application of the Richardson-Lucy total variation algorithm, provided superior image clarity and fidelity in two-and three-dimensions.

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http://hdl.handle.net/10453/139369