Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System.
- Publisher:
- Springer New York
- Publication Type:
- Journal Article
- Citation:
- Methods in molecular biology (Clifton, N.J.), 2019, 1880, pp. 163-172
- Issue Date:
- 2019-01
Closed Access
Filename | Description | Size | |||
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201901 Chen Su et al Autophagy MiMB.pdf | Published version | 375.24 kB | Adobe PDF |
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Full metadata record
Field | Value | Language |
---|---|---|
dc.contributor.author | Chen, Y | |
dc.contributor.author | Su, QP | |
dc.contributor.author | Yu, L | |
dc.date.accessioned | 2020-05-13T04:14:01Z | |
dc.date.available | 2020-05-13T04:14:01Z | |
dc.date.issued | 2019-01 | |
dc.identifier.citation | Methods in molecular biology (Clifton, N.J.), 2019, 1880, pp. 163-172 | |
dc.identifier.isbn | 9781493988723 | |
dc.identifier.issn | 1064-3745 | |
dc.identifier.issn | 1940-6029 | |
dc.identifier.uri | http://hdl.handle.net/10453/140677 | |
dc.description.abstract | Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins. | |
dc.format | ||
dc.language | eng | |
dc.publisher | Springer New York | |
dc.relation.ispartof | Methods in molecular biology (Clifton, N.J.) | |
dc.relation.isbasedon | 10.1007/978-1-4939-8873-0_9 | |
dc.rights | info:eu-repo/semantics/restrictedAccess | |
dc.subject | 0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology | |
dc.subject.classification | Developmental Biology | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Microtubules | |
dc.subject.mesh | Lysosomes | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Rats | |
dc.subject.mesh | Microscopy, Confocal | |
dc.subject.mesh | Cell Fractionation | |
dc.subject.mesh | Autophagy | |
dc.subject.mesh | Molecular Motor Proteins | |
dc.subject.mesh | Animals | |
dc.subject.mesh | Autophagy | |
dc.subject.mesh | Cell Fractionation | |
dc.subject.mesh | Cell Line | |
dc.subject.mesh | Humans | |
dc.subject.mesh | Lysosomes | |
dc.subject.mesh | Microscopy, Confocal | |
dc.subject.mesh | Microtubules | |
dc.subject.mesh | Mitochondria | |
dc.subject.mesh | Molecular Motor Proteins | |
dc.subject.mesh | Rats | |
dc.title | Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System. | |
dc.type | Journal Article | |
utslib.citation.volume | 1880 | |
utslib.location.activity | United States | |
utslib.for | 0399 Other Chemical Sciences | |
utslib.for | 0601 Biochemistry and Cell Biology | |
utslib.for | 0399 Other Chemical Sciences | |
utslib.for | 0601 Biochemistry and Cell Biology | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science | |
pubs.organisational-group | /University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices | |
pubs.organisational-group | /University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences | |
pubs.organisational-group | /University of Technology Sydney | |
utslib.copyright.status | closed_access | * |
dc.date.updated | 2020-05-13T04:13:56Z | |
pubs.publication-status | Published | |
pubs.volume | 1880 | |
utslib.start-page | 163 |
Abstract:
Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins.
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