Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System.

Chen, Y; Su, QP; Yu, L

Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System.

Chen, Y Su, QP Yu, L

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Publisher:: Springer New York
Publication Type:: Journal Article
Citation:: Methods in molecular biology (Clifton, N.J.), 2019, 1880, pp. 163-172
Issue Date:: 2019-01

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Field	Value	Language
dc.contributor.author	Chen, Y
dc.contributor.author	Su, QP
dc.contributor.author	Yu, L
dc.date.accessioned	2020-05-13T04:14:01Z
dc.date.available	2020-05-13T04:14:01Z
dc.date.issued	2019-01
dc.identifier.citation	Methods in molecular biology (Clifton, N.J.), 2019, 1880, pp. 163-172
dc.identifier.isbn	9781493988723
dc.identifier.issn	1064-3745
dc.identifier.issn	1940-6029
dc.identifier.uri	http://hdl.handle.net/10453/140677
dc.description.abstract	Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins.
dc.format	Print
dc.language	eng
dc.publisher	Springer New York
dc.relation.ispartof	Methods in molecular biology (Clifton, N.J.)
dc.relation.isbasedon	10.1007/978-1-4939-8873-0_9
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Cell Line
dc.subject.mesh	Microtubules
dc.subject.mesh	Lysosomes
dc.subject.mesh	Mitochondria
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Rats
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Cell Fractionation
dc.subject.mesh	Autophagy
dc.subject.mesh	Molecular Motor Proteins
dc.subject.mesh	Animals
dc.subject.mesh	Autophagy
dc.subject.mesh	Cell Fractionation
dc.subject.mesh	Cell Line
dc.subject.mesh	Humans
dc.subject.mesh	Lysosomes
dc.subject.mesh	Microscopy, Confocal
dc.subject.mesh	Microtubules
dc.subject.mesh	Mitochondria
dc.subject.mesh	Molecular Motor Proteins
dc.subject.mesh	Rats
dc.title	Studying Autophagic Lysosome Reformation in Cells and by an In Vitro Reconstitution System.
dc.type	Journal Article
utslib.citation.volume	1880
utslib.location.activity	United States
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-05-13T04:13:56Z
pubs.publication-status	Published
pubs.volume	1880
utslib.start-page	163

Abstract:

Autophagic lysosome reformation (ALR) is the terminal step of autophagy. ALR functions to recycle lysosomal membranes and maintain lysosome homeostasis. Maintaining a functional lysosome pool is critical for generating autolysosomes, in which cellular components are degraded and turned over during autophagy. This unit describes methods to visualize ALR in cells. In addition, this unit provides detailed protocols to establish in vitro systems which can be used to reconstitute ALR as well as to reconstitute mitochondrial tubulation/network formation, another process that is driven by motor proteins.

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http://hdl.handle.net/10453/140677