The effect of titanium alloy and stainless steel implants on immunological responses by analysis of NF-κB/ p65, NF-κB1/p50 profiles and the tregs

Akyol, S; Hanci, M; Ben-Nissan, B

The effect of titanium alloy and stainless steel implants on immunological responses by analysis of NF-κB/ p65, NF-κB1/p50 profiles and the tregs

Akyol, S Hanci, M Ben-Nissan, B

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Publication Type:: Journal Article
Citation:: Revista Cubana de Investigaciones Biomedicas, 2019, 38, (5)
Issue Date:: 2019-01-01

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Field	Value	Language
dc.contributor.author	Akyol, S
dc.contributor.author	Hanci, M
dc.contributor.author	Ben-Nissan, B
dc.date.accessioned	2020-05-18T22:19:45Z
dc.date.available	2020-05-18T22:19:45Z
dc.date.issued	2019-01-01
dc.identifier.citation	Revista Cubana de Investigaciones Biomedicas, 2019, 38, (5)
dc.identifier.issn	0864-0300
dc.identifier.issn	1561-3011
dc.identifier.uri	http://hdl.handle.net/10453/140789
dc.description.abstract	© 2019, Editorial Ciencias Medicas. All rights reserved. Introduction: A comprehensive understanding of the tissue-biomaterial interactions at a cellular level is required to explain the immune responses of numerous implant mediated complications and help to improve biomaterial design and use. Objectives: To research the effect of titanium alloy and stainless steel implants on immunological responses in rats by analysis of NF-κB/ p65, NF-κB1/p50 profiles in the activation of inflammatory signaling pathways and the role of Tregs. To minimize the inflammatory response of host-formed bio-material implants is our main goal. Materials and Methods: In this study, 39 Wistar albino male rats were divided into three groups with 13 rats each resulting in Group I (n: 13, sham), Group II (n: 13, Ti alloy rods), and Group III (n: 13, SS alloy rods). The NF-κB/ p65, NF-κB1/p50 and CD4+CD25+Foxp3+ (Tregs) in the blood were analyzed on days 7, 14 and 28 using ELISA and Flow cytometry. Results: Tregs level were lower in the stainless steel (SS) alloy compared to the sham and Titanium (Ti) alloy. NF-κB/ p65 (RelA) levels in the SS alloy showed a significant increase on all days in comparison with the sham and Ti alloy. NF-κB1 (p50) in the SS alloy was a significant increase on the 14th and 28th day. When the Ti alloy was compared with the SS alloy, NF-κB/ p65 (RelA) and NF-κB1 (p50) levels were significantly lower levels. Conclusions: Both the Ti alloy and SS alloy group implantation effects CD4+CD25+Treg cells in different ways. This work suggests that NF-κB/ p65, NF-κB1/p50 have excellent potential as a therapeutic target in the prevention of adverse reactions to metal, especially for controlling the inflammation after the implantation. In this application target can be NF-kB and for this IKK molecule inhibitors can be used or it can be done by the stabilization of IkB proteins.
dc.language	en
dc.relation.ispartof	Revista Cubana de Investigaciones Biomedicas
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Cardiovascular System & Hematology
dc.title	The effect of titanium alloy and stainless steel implants on immunological responses by analysis of NF-κB/ p65, NF-κB1/p50 profiles and the tregs
dc.type	Journal Article
utslib.citation.volume	38
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-05-18T22:19:42Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	38
utslib.citation.issue	5

Abstract:

© 2019, Editorial Ciencias Medicas. All rights reserved. Introduction: A comprehensive understanding of the tissue-biomaterial interactions at a cellular level is required to explain the immune responses of numerous implant mediated complications and help to improve biomaterial design and use. Objectives: To research the effect of titanium alloy and stainless steel implants on immunological responses in rats by analysis of NF-κB/ p65, NF-κB1/p50 profiles in the activation of inflammatory signaling pathways and the role of Tregs. To minimize the inflammatory response of host-formed bio-material implants is our main goal. Materials and Methods: In this study, 39 Wistar albino male rats were divided into three groups with 13 rats each resulting in Group I (n: 13, sham), Group II (n: 13, Ti alloy rods), and Group III (n: 13, SS alloy rods). The NF-κB/ p65, NF-κB1/p50 and CD4+CD25+Foxp3+ (Tregs) in the blood were analyzed on days 7, 14 and 28 using ELISA and Flow cytometry. Results: Tregs level were lower in the stainless steel (SS) alloy compared to the sham and Titanium (Ti) alloy. NF-κB/ p65 (RelA) levels in the SS alloy showed a significant increase on all days in comparison with the sham and Ti alloy. NF-κB1 (p50) in the SS alloy was a significant increase on the 14th and 28th day. When the Ti alloy was compared with the SS alloy, NF-κB/ p65 (RelA) and NF-κB1 (p50) levels were significantly lower levels. Conclusions: Both the Ti alloy and SS alloy group implantation effects CD4+CD25+Treg cells in different ways. This work suggests that NF-κB/ p65, NF-κB1/p50 have excellent potential as a therapeutic target in the prevention of adverse reactions to metal, especially for controlling the inflammation after the implantation. In this application target can be NF-kB and for this IKK molecule inhibitors can be used or it can be done by the stabilization of IkB proteins.

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http://hdl.handle.net/10453/140789