Gradient-sized control of tumor spheroids on a single chip.

Fang, G; Lu, H; Law, A; Gallego-Ortega, D; Jin, D; Lin, G

Gradient-sized control of tumor spheroids on a single chip.

Fang, G Lu, H

Law, A Gallego-Ortega, D Jin, D

Lin, G

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Publisher:: ROYAL SOC CHEMISTRY
Publication Type:: Journal Article
Citation:: Lab on a chip, 2019, 19, (24), pp. 4093-4103
Issue Date:: 2019-12

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Field	Value	Language
dc.contributor.author	Fang, G
dc.contributor.author	Lu, H https://orcid.org/0000-0001-6932-1900
dc.contributor.author	Law, A
dc.contributor.author	Gallego-Ortega, D
dc.contributor.author	Jin, D https://orcid.org/0000-0003-1046-2666
dc.contributor.author	Lin, G https://orcid.org/0000-0001-9880-8478
dc.date.accessioned	2020-05-18T22:36:37Z
dc.date.available	2020-05-18T22:36:37Z
dc.date.issued	2019-12
dc.identifier.citation	Lab on a chip, 2019, 19, (24), pp. 4093-4103
dc.identifier.issn	1473-0197
dc.identifier.issn	1473-0189
dc.identifier.uri	http://hdl.handle.net/10453/140792
dc.description.abstract	Multicellular tumor spheroids are attracting more attention as a physiologically relevant in vitro tumor model for biomedical research. The size of spheroids is one of the critical parameters related to drug penetration and cellular responses. It remains challenging to generate a large number of gradient-sized spheroids in one culture vessel. Here, a liquid-dome method was used to simultaneously produce more than 200 gradient-sized spheroids on an agarose chip. Surface tension effect was used to modulate the liquid spatial distribution and achieve a range of spheroid sizes. MCF-7 cells formed multiple spheroids on the chips for concept validation. It showed that different configurations of the liquid domes exhibited different levels of size control. Relative to the smallest spheroids in the configuration, hemispheric and square domes produced spheroids up to 3.4 and 12.8-fold larger in area, respectively. In addition, the co-culture of MCF-7 and fibroblasts helped to elucidate the tendency of fibroblasts towards the spheroid center. Other size-dependent behaviors were profiled; larger spheroids behaved differently from smaller spheroids in terms of spheroid growth, drug penetration and cellular responses. This method breaks the boundary between the preparation of gradient-sized spheroids and significant time/labour demand. It can be useful for drug screening and in vitro tumor modelling.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	ROYAL SOC CHEMISTRY
dc.relation	http://purl.org/au-research/grants/arc/IH150100028
dc.relation	http://purl.org/au-research/grants/arc/LE180100043
dc.relation.ispartof	Lab on a chip
dc.relation.isbasedon	10.1039/c9lc00872a
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	03 Chemical Sciences, 09 Engineering
dc.subject.classification	Analytical Chemistry
dc.title	Gradient-sized control of tumor spheroids on a single chip.
dc.type	Journal Article
utslib.citation.volume	19
utslib.location.activity	England
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Students
utslib.copyright.status	closed_access	*
dc.date.updated	2020-05-18T22:36:32Z
pubs.issue	24
pubs.publication-status	Published
pubs.volume	19
utslib.start-page	4093
utslib.citation.issue	24

Abstract:

Multicellular tumor spheroids are attracting more attention as a physiologically relevant in vitro tumor model for biomedical research. The size of spheroids is one of the critical parameters related to drug penetration and cellular responses. It remains challenging to generate a large number of gradient-sized spheroids in one culture vessel. Here, a liquid-dome method was used to simultaneously produce more than 200 gradient-sized spheroids on an agarose chip. Surface tension effect was used to modulate the liquid spatial distribution and achieve a range of spheroid sizes. MCF-7 cells formed multiple spheroids on the chips for concept validation. It showed that different configurations of the liquid domes exhibited different levels of size control. Relative to the smallest spheroids in the configuration, hemispheric and square domes produced spheroids up to 3.4 and 12.8-fold larger in area, respectively. In addition, the co-culture of MCF-7 and fibroblasts helped to elucidate the tendency of fibroblasts towards the spheroid center. Other size-dependent behaviors were profiled; larger spheroids behaved differently from smaller spheroids in terms of spheroid growth, drug penetration and cellular responses. This method breaks the boundary between the preparation of gradient-sized spheroids and significant time/labour demand. It can be useful for drug screening and in vitro tumor modelling.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/140792