Zostera virus T - a novel virus of the genus Tepovirus identified in the eelgrass, Zostera muelleri.

Goh, CJ; Park, D; Lee, JS; Davey, PA; Pernice, M; Ralph, PJ; Hahn, Y

Zostera virus T - a novel virus of the genus Tepovirus identified in the eelgrass, Zostera muelleri.

Goh, CJ Park, D Lee, JS Davey, PA Pernice, M

Ralph, PJ Hahn, Y

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Publisher:: AEPRESS SRO
Publication Type:: Journal Article
Citation:: Acta virologica, 2019, 63, (4), pp. 366-372
Issue Date:: 2019-01

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Field	Value	Language
dc.contributor.author	Goh, CJ
dc.contributor.author	Park, D
dc.contributor.author	Lee, JS
dc.contributor.author	Davey, PA
dc.contributor.author	Pernice, M https://orcid.org/0000-0002-3431-2104
dc.contributor.author	Ralph, PJ
dc.contributor.author	Hahn, Y
dc.date.accessioned	2020-05-20T02:12:31Z
dc.date.available	2020-05-20T02:12:31Z
dc.date.issued	2019-01
dc.identifier.citation	Acta virologica, 2019, 63, (4), pp. 366-372
dc.identifier.issn	0001-723X
dc.identifier.issn	1336-2305
dc.identifier.uri	http://hdl.handle.net/10453/140831
dc.description.abstract	Analysis of a transcriptome dataset obtained from tissue samples of the eelgrass Zostera muelleri, an aquatic flowering plant species of the family Zosteraceae, yielded three genome sequence contigs of a novel RNA virus. Sequence comparison and phylogenetic analysis revealed that the novel RNA virus, named Zostera virus T (ZoVT), belongs to the genus Tepovirus of the family Betaflexiviridae. The three genome contigs of ZoVT showed 88.2‒97.2% nucleotide sequence identity to each other, indicating that they descended from a common ancestor. The ZoVT genome contains three open reading frames (ORFs): ORF1 encodes a 1816 amino acid (aa) replicase (REP) with RNA-dependent RNA polymerase (RdRp) activity; ORF2, a 398 aa movement protein (MP); and ORF3, a 240 aa coat protein (CP). The phylogenetic analysis using REP sequences of ZoVT and other Betaflexiviridae viruses showed that Prunus virus T is the closest known virus to ZoVT, whereas potato virus T, the type species of the genus Tepovirus, is the second closest virus. Genome sequences of ZoVT, which is the third tepovirus species identified to date, may be useful for investigating the evolution and molecular biology of tepoviruses. Keywords: Zostera virus T; Tepovirus; Betaflexiviridae; eelgrass; Zostera muelleri.
dc.format	Print
dc.language	eng
dc.publisher	AEPRESS SRO
dc.relation.ispartof	Acta virologica
dc.relation.isbasedon	10.4149/av_2019_409
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0605 Microbiology, 1108 Medical Microbiology
dc.subject.classification	Virology
dc.subject.mesh	Flexiviridae
dc.subject.mesh	Genome, Viral
dc.subject.mesh	Open Reading Frames
dc.subject.mesh	Phylogeny
dc.subject.mesh	RNA, Viral
dc.subject.mesh	Zosteraceae
dc.title	Zostera virus T - a novel virus of the genus Tepovirus identified in the eelgrass, Zostera muelleri.
dc.type	Journal Article
utslib.citation.volume	63
utslib.location.activity	Slovakia
utslib.for	0605 Microbiology
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-05-20T02:12:25Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	63
utslib.start-page	366
utslib.citation.issue	4

Abstract:

Analysis of a transcriptome dataset obtained from tissue samples of the eelgrass Zostera muelleri, an aquatic flowering plant species of the family Zosteraceae, yielded three genome sequence contigs of a novel RNA virus. Sequence comparison and phylogenetic analysis revealed that the novel RNA virus, named Zostera virus T (ZoVT), belongs to the genus Tepovirus of the family Betaflexiviridae. The three genome contigs of ZoVT showed 88.2‒97.2% nucleotide sequence identity to each other, indicating that they descended from a common ancestor. The ZoVT genome contains three open reading frames (ORFs): ORF1 encodes a 1816 amino acid (aa) replicase (REP) with RNA-dependent RNA polymerase (RdRp) activity; ORF2, a 398 aa movement protein (MP); and ORF3, a 240 aa coat protein (CP). The phylogenetic analysis using REP sequences of ZoVT and other Betaflexiviridae viruses showed that Prunus virus T is the closest known virus to ZoVT, whereas potato virus T, the type species of the genus Tepovirus, is the second closest virus. Genome sequences of ZoVT, which is the third tepovirus species identified to date, may be useful for investigating the evolution and molecular biology of tepoviruses. Keywords: Zostera virus T; Tepovirus; Betaflexiviridae; eelgrass; Zostera muelleri.

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http://hdl.handle.net/10453/140831