First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent

Abril, D; Bustos Moya, IG; Marquez-Ortiz, RA; Josa Montero, DF; Corredor Rozo, ZL; Torres Molina, I; Vanegas Gómez, N; Escobar-Perez, J

First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent

Abril, D Bustos Moya, IG Marquez-Ortiz, RA Josa Montero, DF Corredor Rozo, ZL Torres Molina, I Vanegas Gómez, N Escobar-Perez, J

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Antibiotics, 8, (4), pp. 222-222

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Field	Value	Language
dc.contributor.author	Abril, D
dc.contributor.author	Bustos Moya, IG
dc.contributor.author	Marquez-Ortiz, RA
dc.contributor.author	Josa Montero, DF
dc.contributor.author	Corredor Rozo, ZL
dc.contributor.author	Torres Molina, I
dc.contributor.author	Vanegas Gómez, N
dc.contributor.author	Escobar-Perez, J
dc.date.accessioned	2020-06-04T23:30:31Z
dc.date.available	2020-06-04T23:30:31Z
dc.identifier.citation	Antibiotics, 8, (4), pp. 222-222
dc.identifier.issn	2079-6382
dc.identifier.uri	http://hdl.handle.net/10453/141117
dc.description.abstract	<jats:p>The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The blaOXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a blaOXA-244–harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.</jats:p>
dc.language	en
dc.publisher	MDPI AG
dc.relation.ispartof	Antibiotics
dc.relation.isbasedon	10.3390/antibiotics8040222
dc.rights	info:eu-repo/semantics/openAccess
dc.title	First Report and Comparative Genomics Analysis of a blaOXA-244-Harboring Escherichia coli Isolate Recovered in the American Continent
dc.type	Journal Article
utslib.citation.volume	8
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-06-04T23:30:24Z
pubs.issue	4
pubs.publication-status	Published online
pubs.volume	8
utslib.start-page	222
utslib.citation.issue	4

Abstract:

The carbapenemase OXA-244 is a derivate of OXA-48, and its detection is very difficult in laboratories. Here, we report the identification and genomic analysis of an Escherichia coli isolate (28Eco12) harboring the blaOXA-244 gene identified in Colombia, South America. The 28Eco12 isolate was identified during a retrospective study, and it was recovered from a patient treated in Colombia. The complete nucleotide sequence was established using the PacBio platform. A comparative genomics analysis with other blaOXA-244–harboring Escherichia coli strains was performed. The 28Eco12 isolate belonged to sequence type (ST) 38, and its genome was composed of two molecules, a chromosome of 5,343,367 bp and a plasmid of 92,027 bp, which belonged to the incompatibility group IncY and did not harbor resistance genes. The blaOXA-244 gene was chromosomally encoded and mobilized by an ISR1-related Tn6237 composite transposon. Notably, this transposon was inserted and located within a new genomic island. To our knowledge, this is the first report of a blaOXA-244–harboring Escherichia coli isolate in America. Our results suggest that the introduction of the OXA-244-producing E. coli isolate was through clonal expansion of the ST38 pandemic clone. Other isolates producing OXA-244 could be circulating silently in America.

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http://hdl.handle.net/10453/141117