Identification of Bacillus and Yersinia species and hoax agents by protein profiling using microfluidic capillary electrophoresis with peak detection algorithms

Bowman, S; Casares-de-Cal, MÁ; Alvarez-Dios, J; Gomez Tato, A; Roffey, P; Richardson, A; McNevin, D; Gahan, ME

Identification of Bacillus and Yersinia species and hoax agents by protein profiling using microfluidic capillary electrophoresis with peak detection algorithms

Bowman, S Casares-de-Cal, MÁ Alvarez-Dios, J Gomez Tato, A Roffey, P Richardson, A McNevin, D

Gahan, ME

Permalink

Publication Type:: Journal Article
Citation:: Australian Journal of Forensic Sciences, 2019
Issue Date:: 2019-01-01

Recently Added

	Filename	Size
	00450618.2019.1629020.pdf	1.93 MB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Open Access

This item is new to OPUS and is not currently available.

Full metadata record

Field	Value	Language
dc.contributor.author	Bowman, S	en_US
dc.contributor.author	Casares-de-Cal, MÁ	en_US
dc.contributor.author	Alvarez-Dios, J	en_US
dc.contributor.author	Gomez Tato, A	en_US
dc.contributor.author	Roffey, P	en_US
dc.contributor.author	Richardson, A	en_US
dc.contributor.author	McNevin, D https://orcid.org/0000-0003-1665-3367	en_US
dc.contributor.author	Gahan, ME	en_US
dc.date.accessioned	2020-06-11T06:51:16Z
dc.date.accessioned	2020-06-11T06:51:16Z
dc.date.available	2020-06-11T06:51:16Z
dc.date.available	2020-06-11T06:51:16Z
dc.date.issued	2019-01-01	en_US
dc.identifier.citation	Australian Journal of Forensic Sciences, 2019	en_US
dc.identifier.issn	0045-0618	en_US
dc.identifier.uri	http://hdl.handle.net/10453/141345
dc.description.abstract	© 2019, © 2019 Australian Academy of Forensic Sciences. Bacillus anthracis and Yersinia pestis are biological agents that pose an increasing concern to national security if deliberately disseminated. Hoax agents, including suspicious white powders and environmental bacterial species, can also cause disruption. In either scenario it is of high importance to rapidly and accurately identify any suspicious powder as hazardous or hoax. Protein profiling, using microfluidic capillary electrophoresis, provides a rapid (less than 40 minutes), reliable and field-based screening method. Two commonly encountered hoax agents, three Bacillus species (including B. anthracis Sterne strain), two Yersinia species and E. coli were profiled on the Experion™ System (Bio-Rad). Peak detection algorithms were developed for the identification of protein peaks in electropherograms. Boolean logic paths were then employed to predict the electrophoretic pattern of samples. Parameters assessed included variation within and between Experion™ Pro260 chips and the ability to discriminate between samples over time intervals, between operators and between field and laboratory analyses. Classification with optimal Boolean logic paths reported no misclassification with an accuracy of 100% for B. anthracis Sterne strain, B. thuringiensis (powder and culture-based), B. cereus and plain wheat flour. Overall there was 75% correct identification for the eight sample types tested.	en_US
dc.relation.ispartof	Australian Journal of Forensic Sciences	en_US
dc.relation.isbasedon	10.1080/00450618.2019.1629020	en_US
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Legal & Forensic Medicine	en_US
dc.title	Identification of Bacillus and Yersinia species and hoax agents by protein profiling using microfluidic capillary electrophoresis with peak detection algorithms	en_US
dc.type	Journal Article
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
utslib.copyright.status	recently_added	*
pubs.publication-status	Published	en_US

Abstract:

© 2019, © 2019 Australian Academy of Forensic Sciences. Bacillus anthracis and Yersinia pestis are biological agents that pose an increasing concern to national security if deliberately disseminated. Hoax agents, including suspicious white powders and environmental bacterial species, can also cause disruption. In either scenario it is of high importance to rapidly and accurately identify any suspicious powder as hazardous or hoax. Protein profiling, using microfluidic capillary electrophoresis, provides a rapid (less than 40 minutes), reliable and field-based screening method. Two commonly encountered hoax agents, three Bacillus species (including B. anthracis Sterne strain), two Yersinia species and E. coli were profiled on the Experion™ System (Bio-Rad). Peak detection algorithms were developed for the identification of protein peaks in electropherograms. Boolean logic paths were then employed to predict the electrophoretic pattern of samples. Parameters assessed included variation within and between Experion™ Pro260 chips and the ability to discriminate between samples over time intervals, between operators and between field and laboratory analyses. Classification with optimal Boolean logic paths reported no misclassification with an accuracy of 100% for B. anthracis Sterne strain, B. thuringiensis (powder and culture-based), B. cereus and plain wheat flour. Overall there was 75% correct identification for the eight sample types tested.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/141345