Analysis of the Archaeal ESCRT Apparatus.

Samson, RY; Duggin, IG; Bell, SD

Analysis of the Archaeal ESCRT Apparatus.

Samson, RY Duggin, IG Bell, SD

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Publisher:: Springer New York
Publication Type:: Chapter
Citation:: Methods in Molecular Biology, 2019, 1998, pp. 1-11
Issue Date:: 2019-01

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Field	Value	Language
dc.contributor.author	Samson, RY
dc.contributor.author	Duggin, IG
dc.contributor.author	Bell, SD
dc.date.accessioned	2020-06-23T01:56:46Z
dc.date.available	2020-06-23T01:56:46Z
dc.date.issued	2019-01
dc.identifier.citation	Methods in Molecular Biology, 2019, 1998, pp. 1-11
dc.identifier.isbn	9781493994915
dc.identifier.uri	http://hdl.handle.net/10453/141629
dc.description.abstract	Members of the archaeal domain of life that lack homologs of actin and tubulin divide by binary fission in a process that is dependent upon orthologs of eukaryotic ESCRT components. Many of these archaeal organisms are hyperthermophilic acidophiles with unique cell wall structures, which create technical challenges for performing traditional cell biological techniques. Here, we describe the "baby machine" method for synchronizing microorganisms at high temperatures in order to study cell cycle-related processes. We also provide details for fixing, permeabilizing, and staining archaeal cells and ESCRT assemblies for observation by light microscopy.
dc.format	Print
dc.language	en
dc.publisher	Springer New York
dc.relation	http://purl.org/au-research/grants/arc/FT160100010
dc.relation.ispartof	Methods in Molecular Biology
dc.relation.isbasedon	10.1007/978-1-4939-9492-2_1
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0399 Other Chemical Sciences, 0601 Biochemistry and Cell Biology
dc.subject.classification	Developmental Biology
dc.subject.mesh	Sulfolobus acidocaldarius
dc.subject.mesh	Archaeal Proteins
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Cell Cycle
dc.subject.mesh	Endosomal Sorting Complexes Required for Transport
dc.subject.mesh	Archaeal Proteins
dc.subject.mesh	Cell Cycle
dc.subject.mesh	Endosomal Sorting Complexes Required for Transport
dc.subject.mesh	Microscopy, Fluorescence
dc.subject.mesh	Sulfolobus acidocaldarius
dc.title	Analysis of the Archaeal ESCRT Apparatus.
dc.type	Chapter
utslib.citation.volume	1998
utslib.for	0399 Other Chemical Sciences
utslib.for	0601 Biochemistry and Cell Biology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-06-23T01:56:41Z
pubs.publication-status	Published
pubs.volume	1998

Abstract:

Members of the archaeal domain of life that lack homologs of actin and tubulin divide by binary fission in a process that is dependent upon orthologs of eukaryotic ESCRT components. Many of these archaeal organisms are hyperthermophilic acidophiles with unique cell wall structures, which create technical challenges for performing traditional cell biological techniques. Here, we describe the "baby machine" method for synchronizing microorganisms at high temperatures in order to study cell cycle-related processes. We also provide details for fixing, permeabilizing, and staining archaeal cells and ESCRT assemblies for observation by light microscopy.

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http://hdl.handle.net/10453/141629