Molecular Analysis of an IncF ColV-Like Plasmid Lineage That Carries a Complex Resistance Locus with a Trackable Genetic Signature.

McKinnon, J; Roy Chowdhury, P; Djordjevic, SP

Molecular Analysis of an IncF ColV-Like Plasmid Lineage That Carries a Complex Resistance Locus with a Trackable Genetic Signature.

McKinnon, J Roy Chowdhury, P Djordjevic, SP

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Publisher:: Mary Ann Liebert Inc
Publication Type:: Journal Article
Citation:: Microbial drug resistance (Larchmont, N.Y.), 2020, 26, (7), pp. 787-793
Issue Date:: 2020-07

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Field	Value	Language
dc.contributor.author	McKinnon, J
dc.contributor.author	Roy Chowdhury, P
dc.contributor.author	Djordjevic, SP
dc.date.accessioned	2020-08-12T07:15:48Z
dc.date.available	2020-08-12T07:15:48Z
dc.date.issued	2020-07
dc.identifier.citation	Microbial drug resistance (Larchmont, N.Y.), 2020, 26, (7), pp. 787-793
dc.identifier.issn	1076-6294
dc.identifier.issn	1931-8448
dc.identifier.uri	http://hdl.handle.net/10453/142079
dc.description.abstract	IncF ColV plasmids are important plasmid incompatibility group that are currently restricted to the Enterobacteriaceae. These plasmids carry an important repertoire of virulence-associated genes (VAGs) that contribute to the ability of avian pathogenic Escherichia coli to cause disease in poultry. VAGs found on ColV plasmids have also been linked to urosepsis and meningitis in humans but the mechanisms that elicit these disease conditions are not well understood. Recently we described the sequence of a ColV plasmid pSDJ2009-52F that carried the typical repertoire of VAGs and a complex resistance gene locus flanked by IS26, an insertion element that plays an important role in mobilizing antibiotic resistance genes on plasmids and genomic islands. We recovered complete ColV-like plasmid sequences from public databases that shared >80% sequence identity with pSDJ2009-52F in geographically diverse regions of the world over a 20-year timeframe. Previously we noted that pSDJ2009-52F carries a unique genetic signature in the class 1 integron within the complex resistance locus that was presumably created by the action of IS26. Here we show that most ColV-like plasmids that are closely related to pSDJ2009-52F also carry the same signature. Our studies provide insight into how these signature-bearing plasmids and the mobile genetic elements they carry traffic between E. coli sequence types over large geographic distances.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Mary Ann Liebert Inc
dc.relation	NSW Department of Primary Industries
dc.relation.ispartof	Microbial drug resistance (Larchmont, N.Y.)
dc.relation.isbasedon	10.1089/mdr.2019.0277
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences
dc.subject.classification	Microbiology
dc.title	Molecular Analysis of an IncF ColV-Like Plasmid Lineage That Carries a Complex Resistance Locus with a Trackable Genetic Signature.
dc.type	Journal Article
utslib.citation.volume	26
utslib.location.activity	United States
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-08-12T07:15:44Z
pubs.issue	7
pubs.publication-status	Published
pubs.volume	26
utslib.citation.issue	7

Abstract:

IncF ColV plasmids are important plasmid incompatibility group that are currently restricted to the Enterobacteriaceae. These plasmids carry an important repertoire of virulence-associated genes (VAGs) that contribute to the ability of avian pathogenic Escherichia coli to cause disease in poultry. VAGs found on ColV plasmids have also been linked to urosepsis and meningitis in humans but the mechanisms that elicit these disease conditions are not well understood. Recently we described the sequence of a ColV plasmid pSDJ2009-52F that carried the typical repertoire of VAGs and a complex resistance gene locus flanked by IS26, an insertion element that plays an important role in mobilizing antibiotic resistance genes on plasmids and genomic islands. We recovered complete ColV-like plasmid sequences from public databases that shared >80% sequence identity with pSDJ2009-52F in geographically diverse regions of the world over a 20-year timeframe. Previously we noted that pSDJ2009-52F carries a unique genetic signature in the class 1 integron within the complex resistance locus that was presumably created by the action of IS26. Here we show that most ColV-like plasmids that are closely related to pSDJ2009-52F also carry the same signature. Our studies provide insight into how these signature-bearing plasmids and the mobile genetic elements they carry traffic between E. coli sequence types over large geographic distances.

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http://hdl.handle.net/10453/142079