Heritable nanosilver resistance in priority pathogen: a unique genetic adaptation and comparison with ionic silver and antibiotics.

Valentin, E; Bottomley, AL; Chilambi, GS; Harry, EJ; Amal, R; Sotiriou, GA; Rice, SA; Gunawan, C

Heritable nanosilver resistance in priority pathogen: a unique genetic adaptation and comparison with ionic silver and antibiotics.

Valentin, E Bottomley, AL Chilambi, GS Harry, EJ Amal, R Sotiriou, GA Rice, SA Gunawan, C

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Publisher:: Royal Society of Chemistry (RSC)
Publication Type:: Journal Article
Citation:: Nanoscale, 2020, 12, (4), pp. 2384-2392
Issue Date:: 2020-01-13

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Field	Value	Language
dc.contributor.author	Valentin, E
dc.contributor.author	Bottomley, AL
dc.contributor.author	Chilambi, GS
dc.contributor.author	Harry, EJ
dc.contributor.author	Amal, R
dc.contributor.author	Sotiriou, GA
dc.contributor.author	Rice, SA
dc.contributor.author	Gunawan, C https://orcid.org/0000-0002-2957-1347
dc.date.accessioned	2020-08-13T05:40:13Z
dc.date.available	2020-08-13T05:40:13Z
dc.date.issued	2020-01-13
dc.identifier.citation	Nanoscale, 2020, 12, (4), pp. 2384-2392
dc.identifier.issn	2040-3364
dc.identifier.issn	2040-3372
dc.identifier.uri	http://hdl.handle.net/10453/142098
dc.description.abstract	The past decade has seen the incorporation of antimicrobial nanosilver (NAg) into medical devices, and, increasingly, in everyday 'antibacterial' products. With the continued rise of antibiotic resistant bacteria, there are concerns that these priority pathogens will also develop resistance to the extensively commercialized nanoparticle antimicrobials. Herein, this work reports the emergence of stable resistance traits to NAg in the WHO-listed priority pathogen Staphylococcus aureus, which has previously been suggested to have no, or very low, capacity for silver resistance. With no native presence of genetically encoded silver defence mechanisms, the work showed that the bacterium is dependent on mutation of physiologically essential genes, including those involved in nucleotide synthesis and oxidative stress defence. While some mutations were uniquely associated with resistance to NAg, the study also found common mutations that could be protective against both NAg and ionic silver. This is consistent with the observation of NAg/ionic silver cross-resistance. These mutations were detected following withdrawal of the silver exposure, denoting heritable characteristics that allow for spread of the resistance traits even with discontinued silver use. Heritable silver resistance in priority pathogen cautions that these nanoparticle antimicrobials should only be used as needed, to preserve their efficacy for treating infections.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Royal Society of Chemistry (RSC)
dc.relation	http://purl.org/au-research/grants/arc/DP180100474
dc.relation.ispartof	Nanoscale
dc.relation.isbasedon	10.1039/c9nr08424j
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	02 Physical Sciences, 03 Chemical Sciences, 10 Technology
dc.subject.classification	Nanoscience & Nanotechnology
dc.title	Heritable nanosilver resistance in priority pathogen: a unique genetic adaptation and comparison with ionic silver and antibiotics.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	England
utslib.for	02 Physical Sciences
utslib.for	03 Chemical Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-08-13T05:39:59Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	12
utslib.citation.issue	4

Abstract:

The past decade has seen the incorporation of antimicrobial nanosilver (NAg) into medical devices, and, increasingly, in everyday 'antibacterial' products. With the continued rise of antibiotic resistant bacteria, there are concerns that these priority pathogens will also develop resistance to the extensively commercialized nanoparticle antimicrobials. Herein, this work reports the emergence of stable resistance traits to NAg in the WHO-listed priority pathogen Staphylococcus aureus, which has previously been suggested to have no, or very low, capacity for silver resistance. With no native presence of genetically encoded silver defence mechanisms, the work showed that the bacterium is dependent on mutation of physiologically essential genes, including those involved in nucleotide synthesis and oxidative stress defence. While some mutations were uniquely associated with resistance to NAg, the study also found common mutations that could be protective against both NAg and ionic silver. This is consistent with the observation of NAg/ionic silver cross-resistance. These mutations were detected following withdrawal of the silver exposure, denoting heritable characteristics that allow for spread of the resistance traits even with discontinued silver use. Heritable silver resistance in priority pathogen cautions that these nanoparticle antimicrobials should only be used as needed, to preserve their efficacy for treating infections.

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http://hdl.handle.net/10453/142098