Whole Genome Sequencing Analysis of Porcine Faecal Commensal Escherichia coli Carrying Class 1 Integrons from Sows and Their Offspring.

Zingali, T; Reid, CJ; Chapman, TA; Gaio, D; Liu, M; Darling, AE; Djordjevic, SP

Whole Genome Sequencing Analysis of Porcine Faecal Commensal Escherichia coli Carrying Class 1 Integrons from Sows and Their Offspring.

Zingali, T Reid, CJ Chapman, TA Gaio, D Liu, M Darling, AE Djordjevic, SP

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Microorganisms, 2020, 8, (6)
Issue Date:: 2020-06-04

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Field	Value	Language
dc.contributor.author	Zingali, T
dc.contributor.author	Reid, CJ
dc.contributor.author	Chapman, TA
dc.contributor.author	Gaio, D
dc.contributor.author	Liu, M
dc.contributor.author	Darling, AE
dc.contributor.author	Djordjevic, SP
dc.date.accessioned	2020-08-14T06:17:00Z
dc.date.available	2020-06-03
dc.date.available	2020-08-14T06:17:00Z
dc.date.issued	2020-06-04
dc.identifier.citation	Microorganisms, 2020, 8, (6)
dc.identifier.issn	2076-2607
dc.identifier.issn	2076-2607
dc.identifier.uri	http://hdl.handle.net/10453/142107
dc.description.abstract	Intensive pig production systems often rely on the use of antimicrobials and heavy metal feed additives to maintain animal health and welfare. To gain insight into the carriage of antimicrobial resistance genes (ARGs) in the faecal flora of commercially reared healthy swine, we characterised the genome sequences of 117 porcine commensal E. coli that carried the class 1 integrase gene (intI1+). Isolates were sourced from 42 healthy sows and 126 of their offspring from a commercial breeding operation in Australia in 2017. intI1+ E. coli was detected in 28/42 (67%) sows and 90/126 (71%) piglets. Phylogroup A, particularly clonal complex 10, and phylogroup B1 featured prominently in the study collection. ST10, ST20, ST48 and ST361 were the dominant sequence types. Notably, 113/117 isolates (96%) carried three or more ARGs. Genes encoding resistance to -lactams, aminoglycosides, trimethoprim, sulphonamides, tetracyclines and heavy metals were dominant. ARGs encoding resistance to last-line agents, such as carbapenems and third generation cephalosporins, were not detected. IS26, an insertion sequence noted for its ability to capture and mobilise ARGs, was present in 108/117 (92%) intI1+ isolates, and it played a role in determining class 1 integron structure. Our data shows that healthy Australian pig faeces are an important reservoir of multidrug resistant E. coli that carry genes encoding resistance to multiple first-generation antibiotics and virulence-associated genes.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation	http://purl.org/au-research/grants/arc/LP150100912
dc.relation.ispartof	Microorganisms
dc.relation.isbasedon	10.3390/microorganisms8060843
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Whole Genome Sequencing Analysis of Porcine Faecal Commensal Escherichia coli Carrying Class 1 Integrons from Sows and Their Offspring.
dc.type	Journal Article
utslib.citation.volume	8
utslib.location.activity	Switzerland
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-08-14T06:16:46Z
pubs.issue	6
pubs.publication-status	Published
pubs.volume	8
utslib.citation.issue	6

Abstract:

Intensive pig production systems often rely on the use of antimicrobials and heavy metal feed additives to maintain animal health and welfare. To gain insight into the carriage of antimicrobial resistance genes (ARGs) in the faecal flora of commercially reared healthy swine, we characterised the genome sequences of 117 porcine commensal E. coli that carried the class 1 integrase gene (intI1+). Isolates were sourced from 42 healthy sows and 126 of their offspring from a commercial breeding operation in Australia in 2017. intI1+ E. coli was detected in 28/42 (67%) sows and 90/126 (71%) piglets. Phylogroup A, particularly clonal complex 10, and phylogroup B1 featured prominently in the study collection. ST10, ST20, ST48 and ST361 were the dominant sequence types. Notably, 113/117 isolates (96%) carried three or more ARGs. Genes encoding resistance to -lactams, aminoglycosides, trimethoprim, sulphonamides, tetracyclines and heavy metals were dominant. ARGs encoding resistance to last-line agents, such as carbapenems and third generation cephalosporins, were not detected. IS26, an insertion sequence noted for its ability to capture and mobilise ARGs, was present in 108/117 (92%) intI1+ isolates, and it played a role in determining class 1 integron structure. Our data shows that healthy Australian pig faeces are an important reservoir of multidrug resistant E. coli that carry genes encoding resistance to multiple first-generation antibiotics and virulence-associated genes.

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http://hdl.handle.net/10453/142107