Whole genome sequence comparison of avian pathogenic Escherichia coli from acute and chronic salpingitis of egg laying hens.

Poulsen, LL; Kudirkiene, E; Jørgensen, SL; Djordjevic, SP; Cummins, ML; Christensen, JP; Christensen, H; Bisgaard, M; Thøfner, I

Whole genome sequence comparison of avian pathogenic Escherichia coli from acute and chronic salpingitis of egg laying hens.

Poulsen, LL Kudirkiene, E Jørgensen, SL Djordjevic, SP Cummins, ML Christensen, JP Christensen, H Bisgaard, M Thøfner, I

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: BMC veterinary research, 2020, 16, (1), pp. 148
Issue Date:: 2020-05-20

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Field	Value	Language
dc.contributor.author	Poulsen, LL
dc.contributor.author	Kudirkiene, E
dc.contributor.author	Jørgensen, SL
dc.contributor.author	Djordjevic, SP
dc.contributor.author	Cummins, ML
dc.contributor.author	Christensen, JP
dc.contributor.author	Christensen, H
dc.contributor.author	Bisgaard, M
dc.contributor.author	Thøfner, I
dc.date.accessioned	2020-08-14T07:05:24Z
dc.date.available	2020-05-10
dc.date.available	2020-08-14T07:05:24Z
dc.date.issued	2020-05-20
dc.identifier.citation	BMC veterinary research, 2020, 16, (1), pp. 148
dc.identifier.issn	1746-6148
dc.identifier.issn	1746-6148
dc.identifier.uri	http://hdl.handle.net/10453/142111
dc.description.abstract	BACKGROUND:Infection in the oviduct (salpingitis) is the most common bacterial infection in egg laying hens and is mainly caused by Escherichia coli. The disease is responsible for decreased animal welfare, considerable economic loss as well as a risk of horizontal and vertical transmission of pathogenic E. coli. The outcome of salpingitis may be either acute or chronic. It has not yet been clarified whether the pathological manifestation is a result of the characteristics of the E. coli or whether the manifestation is associated with host factors such as host immunity. RESULTS:From the core- and accessory genome analysis and comparison of 62 E. coli no genetic markers were found to be associated to either acute or chronic infection. Twenty of the 62 genomes harboured at least one antimicrobial resistance gene with resistance against sulfonamides being the most common. The increased serum survival and iron chelating genes iss and iroN were highly prevalent in genomes from both acute and chronic salpingitis. CONCLUSION:Our analysis revealed that no genetic markers could differentiate the E. coli isolated from acute versus chronic salpingitis in egg laying hens. The difference in pathological outcome may be related to other factors such as immunological status, genetics and health of the host. These data indicate that salpingitis is another manifestation of colibacillosis.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	BMC veterinary research
dc.relation.isbasedon	10.1186/s12917-020-02369-5
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This is a post-peer-review, pre-copyedit version of an article published in BMC veterinary research. The final authenticated version is available online at: https://dx.doi.org/10.1186/s12917-020-02369-5.	en_US
dc.subject	0601 Biochemistry and Cell Biology, 0605 Microbiology, 0707 Veterinary Sciences
dc.subject.classification	Veterinary Sciences
dc.title	Whole genome sequence comparison of avian pathogenic Escherichia coli from acute and chronic salpingitis of egg laying hens.
dc.type	Journal Article
utslib.citation.volume	16
utslib.location.activity	England
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	0605 Microbiology
utslib.for	0707 Veterinary Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-08-14T07:05:12Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	16
utslib.citation.issue	1

Abstract:

BACKGROUND:Infection in the oviduct (salpingitis) is the most common bacterial infection in egg laying hens and is mainly caused by Escherichia coli. The disease is responsible for decreased animal welfare, considerable economic loss as well as a risk of horizontal and vertical transmission of pathogenic E. coli. The outcome of salpingitis may be either acute or chronic. It has not yet been clarified whether the pathological manifestation is a result of the characteristics of the E. coli or whether the manifestation is associated with host factors such as host immunity. RESULTS:From the core- and accessory genome analysis and comparison of 62 E. coli no genetic markers were found to be associated to either acute or chronic infection. Twenty of the 62 genomes harboured at least one antimicrobial resistance gene with resistance against sulfonamides being the most common. The increased serum survival and iron chelating genes iss and iroN were highly prevalent in genomes from both acute and chronic salpingitis. CONCLUSION:Our analysis revealed that no genetic markers could differentiate the E. coli isolated from acute versus chronic salpingitis in egg laying hens. The difference in pathological outcome may be related to other factors such as immunological status, genetics and health of the host. These data indicate that salpingitis is another manifestation of colibacillosis.

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http://hdl.handle.net/10453/142111