Nanosilver Targets the Bacterial Cell Envelope: The Link with Generation of Reactive Oxygen Radicals.

Gunawan, C; Faiz, MB; Mann, R; Ting, SRS; Sotiriou, GA; Marquis, CP; Amal, R

Nanosilver Targets the Bacterial Cell Envelope: The Link with Generation of Reactive Oxygen Radicals.

Gunawan, C

Faiz, MB Mann, R

Ting, SRS Sotiriou, GA Marquis, CP Amal, R

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: ACS applied materials & interfaces, 2020, 12, (5), pp. 5557-5568
Issue Date:: 2020-02

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Field	Value	Language
dc.contributor.author	Gunawan, C https://orcid.org/0000-0002-2957-1347
dc.contributor.author	Faiz, MB
dc.contributor.author	Mann, R https://orcid.org/0000-0003-4772-0139
dc.contributor.author	Ting, SRS
dc.contributor.author	Sotiriou, GA
dc.contributor.author	Marquis, CP
dc.contributor.author	Amal, R
dc.date.accessioned	2020-08-17T01:59:46Z
dc.date.available	2020-08-17T01:59:46Z
dc.date.issued	2020-02
dc.identifier.citation	ACS applied materials & interfaces, 2020, 12, (5), pp. 5557-5568
dc.identifier.issn	1944-8244
dc.identifier.issn	1944-8252
dc.identifier.uri	http://hdl.handle.net/10453/142137
dc.description.abstract	The work describes the interactions of nanosilver (NAg) with bacterial cell envelope components at a molecular level and how this associates with the reactive oxygen species (ROS)-mediated toxicity of the nanoparticle. Major structural changes were detected in cell envelope biomolecules as a result of damages in functional moieties, such as the saccharides, amides, and phosphodiesters. NAg exposure disintegrates the glycan backbone in the major cell wall component peptidoglycan, causes complete breakdown of lipoteichoic acid, and disrupts the phosphate-amine and fatty acid groups in phosphatidylethanolamine, a membrane phospholipid. Consistent with the oxidative attacks, we propose that the observed cell envelope damages are inflicted, at least in part, by the reactive oxygen radicals being generated by the nanoparticle during its leaching process, abiotically, without cells. The cell envelope targeting, especially those on the inner membrane phospholipid, is likely to then trigger the rapid generation of lethal levels of cellular superoxide (O2•-) and hydroxyl (OH•) radicals herein seen with a model bacterium. The present study provides a better understanding of the antibacterial mechanisms of NAg, whereby ROS generation could be both the cause and consequence of the toxicity, associated with the initial cell envelope targeting by the nanoparticle.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation	http://purl.org/au-research/grants/arc/DP180100474
dc.relation.ispartof	ACS applied materials & interfaces
dc.relation.hasversion	Accepted version	en
dc.relation.isbasedon	10.1021/acsami.9b20193
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This document is the Accepted Manuscript version of a Published Work that appeared in final form in ACS Applied Materials and Interfaces, copyright © American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://doi.org/10.1021/acsami.9b20193	en
dc.subject	03 Chemical Sciences, 09 Engineering
dc.subject.classification	Nanoscience & Nanotechnology
dc.title	Nanosilver Targets the Bacterial Cell Envelope: The Link with Generation of Reactive Oxygen Radicals.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	09 Engineering
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-08-17T01:59:21Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	12
utslib.citation.issue	5

Abstract:

The work describes the interactions of nanosilver (NAg) with bacterial cell envelope components at a molecular level and how this associates with the reactive oxygen species (ROS)-mediated toxicity of the nanoparticle. Major structural changes were detected in cell envelope biomolecules as a result of damages in functional moieties, such as the saccharides, amides, and phosphodiesters. NAg exposure disintegrates the glycan backbone in the major cell wall component peptidoglycan, causes complete breakdown of lipoteichoic acid, and disrupts the phosphate-amine and fatty acid groups in phosphatidylethanolamine, a membrane phospholipid. Consistent with the oxidative attacks, we propose that the observed cell envelope damages are inflicted, at least in part, by the reactive oxygen radicals being generated by the nanoparticle during its leaching process, abiotically, without cells. The cell envelope targeting, especially those on the inner membrane phospholipid, is likely to then trigger the rapid generation of lethal levels of cellular superoxide (O2•-) and hydroxyl (OH•) radicals herein seen with a model bacterium. The present study provides a better understanding of the antibacterial mechanisms of NAg, whereby ROS generation could be both the cause and consequence of the toxicity, associated with the initial cell envelope targeting by the nanoparticle.

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http://hdl.handle.net/10453/142137