An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata.

Aili, SR; Touchard, A; Hayward, R; Robinson, SD; Pineda, SS; Lalagüe, H; Mrinalini; Vetter, I; Undheim, EAB; Kini, RM; Escoubas, P; Padula, MP; Myers, GSA; Nicholson, GM

An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata.

Aili, SR Touchard, A Hayward, R

Robinson, SD Pineda, SS Lalagüe, H Mrinalini Vetter, I Undheim, EAB Kini, RM Escoubas, P Padula, MP Myers, GSA Nicholson, GM

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Toxins, 2020, 12, (5)
Issue Date:: 2020-05-14

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Field	Value	Language
dc.contributor.author	Aili, SR
dc.contributor.author	Touchard, A
dc.contributor.author	Hayward, R https://orcid.org/0000-0002-6300-3271
dc.contributor.author	Robinson, SD
dc.contributor.author	Pineda, SS
dc.contributor.author	Lalagüe, H
dc.contributor.author	Mrinalini
dc.contributor.author	Vetter, I
dc.contributor.author	Undheim, EAB
dc.contributor.author	Kini, RM
dc.contributor.author	Escoubas, P
dc.contributor.author	Padula, MP
dc.contributor.author	Myers, GSA
dc.contributor.author	Nicholson, GM
dc.date.accessioned	2020-08-18T06:05:12Z
dc.date.available	2020-05-12
dc.date.available	2020-08-18T06:05:12Z
dc.date.issued	2020-05-14
dc.identifier.citation	Toxins, 2020, 12, (5)
dc.identifier.issn	2072-6651
dc.identifier.issn	2072-6651
dc.identifier.uri	http://hdl.handle.net/10453/142196
dc.description.abstract	A critical hurdle in ant venom proteomic investigations is the lack of databases to comprehensively and specifically identify the sequence and function of venom proteins and peptides. To resolve this, we used venom gland transcriptomics to generate a sequence database that was used to assign the tandem mass spectrometry (MS) fragmentation spectra of venom peptides and proteins to specific transcripts. This was performed alongside a shotgun liquid chromatography-mass spectrometry (LC-MS/MS) analysis of the venom to confirm that these assigned transcripts were expressed as proteins. Through the combined transcriptomic and proteomic investigation of Paraponera clavata venom, we identified four times the number of proteins previously identified using 2D-PAGE alone. In addition to this, by mining the transcriptomic data, we identified several novel peptide sequences for future pharmacological investigations, some of which conform with inhibitor cysteine knot motifs. These types of peptides have the potential to be developed into pharmaceutical or bioinsecticide peptides.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI
dc.relation.ispartof	Toxins
dc.relation.isbasedon	10.3390/toxins12050324
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0601 Biochemistry and Cell Biology, 1115 Pharmacology and Pharmaceutical Sciences
dc.title	An Integrated Proteomic and Transcriptomic Analysis Reveals the Venom Complexity of the Bullet Ant Paraponera clavata.
dc.type	Journal Article
utslib.citation.volume	12
utslib.location.activity	Switzerland
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
dc.date.updated	2020-08-18T06:04:40Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	12
utslib.citation.issue	5

Abstract:

A critical hurdle in ant venom proteomic investigations is the lack of databases to comprehensively and specifically identify the sequence and function of venom proteins and peptides. To resolve this, we used venom gland transcriptomics to generate a sequence database that was used to assign the tandem mass spectrometry (MS) fragmentation spectra of venom peptides and proteins to specific transcripts. This was performed alongside a shotgun liquid chromatography-mass spectrometry (LC-MS/MS) analysis of the venom to confirm that these assigned transcripts were expressed as proteins. Through the combined transcriptomic and proteomic investigation of Paraponera clavata venom, we identified four times the number of proteins previously identified using 2D-PAGE alone. In addition to this, by mining the transcriptomic data, we identified several novel peptide sequences for future pharmacological investigations, some of which conform with inhibitor cysteine knot motifs. These types of peptides have the potential to be developed into pharmaceutical or bioinsecticide peptides.

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http://hdl.handle.net/10453/142196