Sexual reproduction and genetic polymorphism within the cosmopolitan marine diatom Pseudo-nitzschia pungens.

Kim, JH; Ajani, P; Murray, SA; Kim, J-H; Lim, HC; Teng, ST; Lim, PT; Han, M-S; Park, BS

Sexual reproduction and genetic polymorphism within the cosmopolitan marine diatom Pseudo-nitzschia pungens.

Kim, JH Ajani, P

Murray, SA Kim, J-H Lim, HC Teng, ST Lim, PT Han, M-S Park, BS

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: Scientific reports, 2020, 10, (1), pp. 10653
Issue Date:: 2020-06-30

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Field	Value	Language
dc.contributor.author	Kim, JH
dc.contributor.author	Ajani, P https://orcid.org/0000-0001-5364-9936
dc.contributor.author	Murray, SA
dc.contributor.author	Kim, J-H
dc.contributor.author	Lim, HC
dc.contributor.author	Teng, ST
dc.contributor.author	Lim, PT
dc.contributor.author	Han, M-S
dc.contributor.author	Park, BS
dc.date.accessioned	2020-09-10T22:20:06Z
dc.date.available	2020-06-10
dc.date.available	2020-09-10T22:20:06Z
dc.date.issued	2020-06-30
dc.identifier.citation	Scientific reports, 2020, 10, (1), pp. 10653
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/142612
dc.description.abstract	Different clades belonging to the cosmopolitan marine diatom Pseudo-nitzschia pungens appear to be present in different oceanic environments, however, a 'hybrid zone', where populations of different clades interbreed, has also been reported. Many studies have investigated the sexual reproduction of P. pungens, focused on morphology and life cycle, rather than the role of sexual reproduction in mixing the genomes of their parents. We carried out crossing experiments to determine the sexual compatibility/incompatibility between different clades of P. pungens, and examined the genetic polymorphism in the ITS2 region. Sexual reproduction did not occur only between clades II and III under any of experimental temperature conditions. Four offspring strains were established between clade I and III successfully. Strains established from offspring were found interbreed with other offspring strains as well as viable with their parental strains. We confirmed the hybrid sequence patterns between clades I and III and found novel sequence types including polymorphic single nucleotide polymorphisms (SNPs) in the offspring strains. Our results implicate that gene exchange and mixing between different clades are still possible, and that sexual reproduction is a significant ecological strategy to maintain the genetic diversity within this diatom species.
dc.format	Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	Scientific reports
dc.relation.isbasedon	10.1038/s41598-020-67547-9
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This is a post-peer-review, pre-copyedit version of an article published in Scientific reports. The final authenticated version is available online at: https://dx.doi.org/10.1038/s41598-020-67547-9.	en_US
dc.title	Sexual reproduction and genetic polymorphism within the cosmopolitan marine diatom Pseudo-nitzschia pungens.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-09-10T22:19:53Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	1

Abstract:

Different clades belonging to the cosmopolitan marine diatom Pseudo-nitzschia pungens appear to be present in different oceanic environments, however, a 'hybrid zone', where populations of different clades interbreed, has also been reported. Many studies have investigated the sexual reproduction of P. pungens, focused on morphology and life cycle, rather than the role of sexual reproduction in mixing the genomes of their parents. We carried out crossing experiments to determine the sexual compatibility/incompatibility between different clades of P. pungens, and examined the genetic polymorphism in the ITS2 region. Sexual reproduction did not occur only between clades II and III under any of experimental temperature conditions. Four offspring strains were established between clade I and III successfully. Strains established from offspring were found interbreed with other offspring strains as well as viable with their parental strains. We confirmed the hybrid sequence patterns between clades I and III and found novel sequence types including polymorphic single nucleotide polymorphisms (SNPs) in the offspring strains. Our results implicate that gene exchange and mixing between different clades are still possible, and that sexual reproduction is a significant ecological strategy to maintain the genetic diversity within this diatom species.

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http://hdl.handle.net/10453/142612