Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins.

Touchard, A; Aili, SR; Téné, N; Barassé, V; Klopp, C; Dejean, A; Kini, RM; Mrinalini; Coquet, L; Jouenne, T; Lefranc, B; Leprince, J; Escoubas, P; Nicholson, GM; Treilhou, M; Bonnafé, E

Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins.

Touchard, A Aili, SR Téné, N Barassé, V Klopp, C Dejean, A Kini, RM Mrinalini Coquet, L Jouenne, T Lefranc, B Leprince, J Escoubas, P Nicholson, GM Treilhou, M Bonnafé, E

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Journal of proteome research, 2020, 19, (4), pp. 1800-1811
Issue Date:: 2020-04

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Field	Value	Language
dc.contributor.author	Touchard, A
dc.contributor.author	Aili, SR
dc.contributor.author	Téné, N
dc.contributor.author	Barassé, V
dc.contributor.author	Klopp, C
dc.contributor.author	Dejean, A
dc.contributor.author	Kini, RM
dc.contributor.author	Mrinalini
dc.contributor.author	Coquet, L
dc.contributor.author	Jouenne, T
dc.contributor.author	Lefranc, B
dc.contributor.author	Leprince, J
dc.contributor.author	Escoubas, P
dc.contributor.author	Nicholson, GM
dc.contributor.author	Treilhou, M
dc.contributor.author	Bonnafé, E
dc.date.accessioned	2020-10-15T23:36:47Z
dc.date.available	2020-10-15T23:36:47Z
dc.date.issued	2020-04
dc.identifier.citation	Journal of proteome research, 2020, 19, (4), pp. 1800-1811
dc.identifier.issn	1535-3893
dc.identifier.issn	1535-3907
dc.identifier.uri	http://hdl.handle.net/10453/143285
dc.description.abstract	Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-, U13-, U20-MYRTX-Mri1a, U10-MYRTX-Mri1b, and U10-MYRTX-Mri1c) with an insecticidal activity. Chemically synthesized U10-MYRTX-Mri1a, -Mri1b, -Mri1c, and U20-MYRTX-Mri1a irreversibly paralyzed blowflies at the highest doses tested (30-125 nmol·g-1). U13-MYRTX-Mri1a, the most potent neurotoxic peptide at 1 h, had reversible effects after 24 h (150 nmol·g-1). Finally, U3-MYRTX-Mri1a has no insecticidal activity, even at up to 55 nmol·g-1. Thus, M. rubida employs a paralytic venom rich in linear insecticidal peptides, which likely act by disrupting cell membranes.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	Journal of proteome research
dc.relation.isbasedon	10.1021/acs.jproteome.0c00048
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	03 Chemical Sciences, 06 Biological Sciences
dc.subject.classification	Biochemistry & Molecular Biology
dc.title	Venom Peptide Repertoire of the European Myrmicine Ant Manica rubida: Identification of Insecticidal Toxins.
dc.type	Journal Article
utslib.citation.volume	19
utslib.location.activity	United States
utslib.for	03 Chemical Sciences
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-10-15T23:36:32Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	19
utslib.citation.issue	4

Abstract:

Using an integrated transcriptomic and proteomic approach, we characterized the venom peptidome of the European red ant, Manica rubida. We identified 13 "myrmicitoxins" that share sequence similarities with previously identified ant venom peptides, one of them being identified as an EGF-like toxin likely resulting from a threonine residue modified by O-fucosylation. Furthermore, we conducted insecticidal assays of reversed-phase HPLC venom fractions on the blowfly Lucilia caesar, permitting us to identify six myrmicitoxins (i.e., U3-, U10-, U13-, U20-MYRTX-Mri1a, U10-MYRTX-Mri1b, and U10-MYRTX-Mri1c) with an insecticidal activity. Chemically synthesized U10-MYRTX-Mri1a, -Mri1b, -Mri1c, and U20-MYRTX-Mri1a irreversibly paralyzed blowflies at the highest doses tested (30-125 nmol·g-1). U13-MYRTX-Mri1a, the most potent neurotoxic peptide at 1 h, had reversible effects after 24 h (150 nmol·g-1). Finally, U3-MYRTX-Mri1a has no insecticidal activity, even at up to 55 nmol·g-1. Thus, M. rubida employs a paralytic venom rich in linear insecticidal peptides, which likely act by disrupting cell membranes.

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http://hdl.handle.net/10453/143285