A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater.

Ruprecht, JE; Glamore, WC; Dafforn, KA; Wemheuer, F; Crane, SL; van Dorst, J; Johnston, EL; Mitrovic, SM; Turner, IL; Ferrari, BC; Birrer, SC

A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater.

Ruprecht, JE Glamore, WC Dafforn, KA Wemheuer, F Crane, SL van Dorst, J Johnston, EL Mitrovic, SM Turner, IL Ferrari, BC Birrer, SC

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Scientific data, 2020, 7, (1), pp. 158
Issue Date:: 2020-05-27

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Field	Value	Language
dc.contributor.author	Ruprecht, JE
dc.contributor.author	Glamore, WC
dc.contributor.author	Dafforn, KA
dc.contributor.author	Wemheuer, F
dc.contributor.author	Crane, SL
dc.contributor.author	van Dorst, J
dc.contributor.author	Johnston, EL
dc.contributor.author	Mitrovic, SM
dc.contributor.author	Turner, IL
dc.contributor.author	Ferrari, BC
dc.contributor.author	Birrer, SC
dc.date.accessioned	2020-10-18T22:43:23Z
dc.date.available	2020-04-20
dc.date.available	2020-10-18T22:43:23Z
dc.date.issued	2020-05-27
dc.identifier.citation	Scientific data, 2020, 7, (1), pp. 158
dc.identifier.issn	2052-4463
dc.identifier.issn	2052-4463
dc.identifier.uri	http://hdl.handle.net/10453/143355
dc.description.abstract	Real-world observational datasets that record and quantify pressure-stressor-response linkages between effluent discharges and natural aquatic systems are rare. With global wastewater volumes increasing at unprecedented rates, it is urgent that the present dataset is available to provide the necessary information about microbial community structure and functioning. Field studies were performed at two time-points in the Austral summer. Single-species and microbial community whole effluent toxicity (WET) testing was performed at a complete range of effluent concentrations and two salinities, with accompanying environmental data to provide new insights into nutrient and organic matter cycling, and to identify ecotoxicological tipping points. The two salinity regimes were chosen to investigate future scenarios based on a predicted salinity increase at the study site, typical of coastal regions with rising sea levels globally. Flow cytometry, amplicon sequencing of 16S and 18S rRNA genes and micro-fluidic quantitative polymerase-chain reactions (MFQPCR) were used to determine chlorophyll-a and total bacterial cell numbers and size, as well as taxonomic and functional diversity of pelagic microbial communities. This strong pilot dataset could be replicated in other regions globally and would be of high value to scientists and engineers to support the next advances in microbial ecotoxicology, environmental biomonitoring and estuarine water quality modelling.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation	Hunter Water Corporation
dc.relation.ispartof	Scientific data
dc.relation.isbasedon	10.1038/s41597-020-0496-5
dc.rights	info:eu-repo/semantics/openAccess
dc.title	A novel real-world ecotoxicological dataset of pelagic microbial community responses to wastewater.
dc.type	Journal Article
utslib.citation.volume	7
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-10-18T22:43:13Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	7
utslib.citation.issue	1

Abstract:

Real-world observational datasets that record and quantify pressure-stressor-response linkages between effluent discharges and natural aquatic systems are rare. With global wastewater volumes increasing at unprecedented rates, it is urgent that the present dataset is available to provide the necessary information about microbial community structure and functioning. Field studies were performed at two time-points in the Austral summer. Single-species and microbial community whole effluent toxicity (WET) testing was performed at a complete range of effluent concentrations and two salinities, with accompanying environmental data to provide new insights into nutrient and organic matter cycling, and to identify ecotoxicological tipping points. The two salinity regimes were chosen to investigate future scenarios based on a predicted salinity increase at the study site, typical of coastal regions with rising sea levels globally. Flow cytometry, amplicon sequencing of 16S and 18S rRNA genes and micro-fluidic quantitative polymerase-chain reactions (MFQPCR) were used to determine chlorophyll-a and total bacterial cell numbers and size, as well as taxonomic and functional diversity of pelagic microbial communities. This strong pilot dataset could be replicated in other regions globally and would be of high value to scientists and engineers to support the next advances in microbial ecotoxicology, environmental biomonitoring and estuarine water quality modelling.

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http://hdl.handle.net/10453/143355