The unique sterol biosynthesis pathway of three model diatoms consists of a conserved core and diversified endpoints

Jaramillo-Madrid, AC; Ashworth, J; Fabris, M; Ralph, PJ

The unique sterol biosynthesis pathway of three model diatoms consists of a conserved core and diversified endpoints

Jaramillo-Madrid, AC Ashworth, J

Fabris, M

Ralph, PJ

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Publisher:: ELSEVIER
Publication Type:: Journal Article
Citation:: Algal Research, 2020, 48
Issue Date:: 2020-06-01

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Field	Value	Language
dc.contributor.author	Jaramillo-Madrid, AC
dc.contributor.author	Ashworth, J https://orcid.org/0000-0003-4835-6551
dc.contributor.author	Fabris, M https://orcid.org/0000-0003-2910-2089
dc.contributor.author	Ralph, PJ
dc.date.accessioned	2020-10-20T03:02:36Z
dc.date.available	2020-10-20T03:02:36Z
dc.date.issued	2020-06-01
dc.identifier.citation	Algal Research, 2020, 48
dc.identifier.issn	2211-9264
dc.identifier.issn	2211-9264
dc.identifier.uri	http://hdl.handle.net/10453/143392
dc.description.abstract	© 2020 Diatoms produce a wide diversity of sterols among different species, the biosynthesis and conservation of which is not yet fully understood. To investigate the conservation and divergence of sterol biosynthesis pathways among diatoms, we performed comparative metabolic profiling and transcriptomics for a centric diatom (Thalassiosira pseudonana), a pennate diatom (Phaeodactylum tricornutum) and a chaetocerid (Chaetoceros muelleri) in response to inhibitors of enzymes involved in sterol biosynthesis. These three model diatoms, which are representative of distinct clades, share a unique core phytosterol biosynthesis pathway that relies on a terbinafine-insensitive alternative squalene epoxidase and the cyclization of 2,3-epoxysqualene into cycloartenol by a conserved oxidosqualene cyclase. Lineage-specific divergence in the synthesis of sterol precursors was found in the species analyzed. Cholesterol synthesis in diatoms seems to occur via cycloartenol rather than lanosterol. The diversification of natural sterols produced by each species appears to occur downstream of all experimentally targeted enzymes, suggesting adaptive specialization in terminal synthesis pathways.
dc.language	English
dc.publisher	ELSEVIER
dc.relation	http://purl.org/au-research/grants/arc/DE160100615
dc.relation.ispartof	Algal Research
dc.relation.isbasedon	10.1016/j.algal.2020.101902
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0607 Plant Biology, 0904 Chemical Engineering, 1003 Industrial Biotechnology
dc.title	The unique sterol biosynthesis pathway of three model diatoms consists of a conserved core and diversified endpoints
dc.type	Journal Article
utslib.citation.volume	48
utslib.for	0607 Plant Biology
utslib.for	0904 Chemical Engineering
utslib.for	1003 Industrial Biotechnology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-10-20T03:02:27Z
pubs.publication-status	Published
pubs.volume	48

Abstract:

© 2020 Diatoms produce a wide diversity of sterols among different species, the biosynthesis and conservation of which is not yet fully understood. To investigate the conservation and divergence of sterol biosynthesis pathways among diatoms, we performed comparative metabolic profiling and transcriptomics for a centric diatom (Thalassiosira pseudonana), a pennate diatom (Phaeodactylum tricornutum) and a chaetocerid (Chaetoceros muelleri) in response to inhibitors of enzymes involved in sterol biosynthesis. These three model diatoms, which are representative of distinct clades, share a unique core phytosterol biosynthesis pathway that relies on a terbinafine-insensitive alternative squalene epoxidase and the cyclization of 2,3-epoxysqualene into cycloartenol by a conserved oxidosqualene cyclase. Lineage-specific divergence in the synthesis of sterol precursors was found in the species analyzed. Cholesterol synthesis in diatoms seems to occur via cycloartenol rather than lanosterol. The diversification of natural sterols produced by each species appears to occur downstream of all experimentally targeted enzymes, suggesting adaptive specialization in terminal synthesis pathways.

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http://hdl.handle.net/10453/143392