A simple spectrophotometric method for rapid quantitative screening of arsenic bio-transforming bacteria

Bahar, MM; Mahbub, KR; Naidu, R; Megharaj, M

A simple spectrophotometric method for rapid quantitative screening of arsenic bio-transforming bacteria

Bahar, MM Mahbub, KR Naidu, R Megharaj, M

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Environmental Technology and Innovation, 2020, 19, pp. 100840-100840
Issue Date:: 2020-08-01

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Field	Value	Language
dc.contributor.author	Bahar, MM
dc.contributor.author	Mahbub, KR
dc.contributor.author	Naidu, R
dc.contributor.author	Megharaj, M
dc.date.accessioned	2020-10-20T19:49:00Z
dc.date.available	2020-10-20T19:49:00Z
dc.date.issued	2020-08-01
dc.identifier.citation	Environmental Technology and Innovation, 2020, 19, pp. 100840-100840
dc.identifier.issn	2352-1864
dc.identifier.issn	2352-1864
dc.identifier.uri	http://hdl.handle.net/10453/143414
dc.description.abstract	© 2020 A simple spectrophotometric technique for inorganic arsenic speciation of known total arsenic has been developed for application in quantitative screening of arsenite-oxidizing and arsenate-reducing bacteria from the wide range of environmental isolates. The technique is based on the spectrophotometric absorbance measurement of the color intensity in the arsenic and potassium permanganate mixture. Since arsenate does not react with potassium permanganate, the intensity of color decreases proportionately with the increasing concentration of arsenite in the total arsenic. The speciation method can be used over a pH range of 4–9. The validity of the results obtained from this spectrophotometric method was confirmed with the Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry (LC-ICP-MS). This method has great potential for the screening of arsenite-oxidizing bacteria.
dc.language	en
dc.publisher	Elsevier BV
dc.relation.ispartof	Environmental Technology and Innovation
dc.relation.isbasedon	10.1016/j.eti.2020.100840
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0502 Environmental Science and Management, 0907 Environmental Engineering, 1002 Environmental Biotechnology
dc.title	A simple spectrophotometric method for rapid quantitative screening of arsenic bio-transforming bacteria
dc.type	Journal Article
utslib.citation.volume	19
utslib.for	0502 Environmental Science and Management
utslib.for	0907 Environmental Engineering
utslib.for	1002 Environmental Biotechnology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-10-20T19:48:46Z
pubs.publication-status	Published
pubs.volume	19

Abstract:

© 2020 A simple spectrophotometric technique for inorganic arsenic speciation of known total arsenic has been developed for application in quantitative screening of arsenite-oxidizing and arsenate-reducing bacteria from the wide range of environmental isolates. The technique is based on the spectrophotometric absorbance measurement of the color intensity in the arsenic and potassium permanganate mixture. Since arsenate does not react with potassium permanganate, the intensity of color decreases proportionately with the increasing concentration of arsenite in the total arsenic. The speciation method can be used over a pH range of 4–9. The validity of the results obtained from this spectrophotometric method was confirmed with the Liquid Chromatography-Inductively Coupled Plasma-Mass Spectrometry (LC-ICP-MS). This method has great potential for the screening of arsenite-oxidizing bacteria.

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http://hdl.handle.net/10453/143414