Sample extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method development and validation for the quantitative detection of cyanobacterial hepatotoxins and neurotoxins in Singapore's reservoirs

Abbas, F; Porojan, C; Mowe, MAD; Lehane, M; Mitrovic, SM; Lim, RP; Yeo, DCJ; Furey, A

Sample extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method development and validation for the quantitative detection of cyanobacterial hepatotoxins and neurotoxins in Singapore's reservoirs

Abbas, F Porojan, C Mowe, MAD Lehane, M Mitrovic, SM Lim, RP Yeo, DCJ Furey, A

Permalink

Publisher:: CSIRO PUBLISHING
Publication Type:: Journal Article
Citation:: Marine and Freshwater Research, 2020, 71, (5), pp. 673-688
Issue Date:: 2020-01-01

Closed Access

	Filename	Description	Size
	MF19157.pdf	Published version	502.24 kB	Adobe PDF	View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Abbas, F
dc.contributor.author	Porojan, C
dc.contributor.author	Mowe, MAD
dc.contributor.author	Lehane, M
dc.contributor.author	Mitrovic, SM
dc.contributor.author	Lim, RP
dc.contributor.author	Yeo, DCJ
dc.contributor.author	Furey, A
dc.date.accessioned	2020-10-30T04:51:37Z
dc.date.available	2020-10-30T04:51:37Z
dc.date.issued	2020-01-01
dc.identifier.citation	Marine and Freshwater Research, 2020, 71, (5), pp. 673-688
dc.identifier.issn	1323-1650
dc.identifier.issn	0067-1940
dc.identifier.uri	http://hdl.handle.net/10453/143618
dc.description.abstract	© 2020 CSIRO. Cyanobacterial blue-green algal toxins are produced by harmful algal blooms (HABs). Most species of phytoplankton are not harmful, but excessive amounts of certain HAB taxa can cause harm to human and animal health, aquatic ecosystems and local economies. To investigate the prevalence of cylindrospermopsin (CYN) and anatoxin-a (ANA) in Singapore's reservoirs, a hazard analysis was initiated to profile the CYN and ANA levels present. Water samples from 17 reservoirs were monitored monthly over a 12-month period (November 2012-October 2013). Analyses were conducted by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a triple-stage quadrupole mass spectrometer with a turbo-assisted ion spray source. CYN was more prevalent than ANA. Intracellular CYN concentrations exceeded 0.4 μg L-1 in 6 of 17 man-made reservoirs surveyed, and slightly exceeded the provisional CYN drinking water guidelines of 1 μg L-1 (National Health and Medical Research Council and National Resource Management Ministerial Council 2011) on one occasion (1.1 μg L-1, July 2013) in one reservoir. The dominant cyanobacteria genera during that period were Cylindrospermopsis, Planktolyngbya, Pseudanabaena and Microcystis. For ANA, all 17 reservoirs had concentrations below 0.1 μg L-1. Based on random forest analysis, the most important environmental factors affecting CYN concentrations were total nitrogen (most important), nitrate, total phosphorus and Cylindrospermopsis counts (least important). The findings of this study indicate that reducing total nitrogen concentrations may be useful in minimising CYN concentrations in tropical reservoirs.
dc.language	English
dc.publisher	CSIRO PUBLISHING
dc.relation.ispartof	Marine and Freshwater Research
dc.relation.isbasedon	10.1071/MF19157
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Marine Biology & Hydrobiology
dc.title	Sample extraction and liquid chromatography-tandem mass spectrometry (LC-MS/MS) method development and validation for the quantitative detection of cyanobacterial hepatotoxins and neurotoxins in Singapore's reservoirs
dc.type	Journal Article
utslib.citation.volume	71
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-10-30T04:51:26Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	71
utslib.citation.issue	5

Abstract:

© 2020 CSIRO. Cyanobacterial blue-green algal toxins are produced by harmful algal blooms (HABs). Most species of phytoplankton are not harmful, but excessive amounts of certain HAB taxa can cause harm to human and animal health, aquatic ecosystems and local economies. To investigate the prevalence of cylindrospermopsin (CYN) and anatoxin-a (ANA) in Singapore's reservoirs, a hazard analysis was initiated to profile the CYN and ANA levels present. Water samples from 17 reservoirs were monitored monthly over a 12-month period (November 2012-October 2013). Analyses were conducted by liquid chromatography-tandem mass spectrometry (LC-MS/MS) using a triple-stage quadrupole mass spectrometer with a turbo-assisted ion spray source. CYN was more prevalent than ANA. Intracellular CYN concentrations exceeded 0.4 μg L-1 in 6 of 17 man-made reservoirs surveyed, and slightly exceeded the provisional CYN drinking water guidelines of 1 μg L-1 (National Health and Medical Research Council and National Resource Management Ministerial Council 2011) on one occasion (1.1 μg L-1, July 2013) in one reservoir. The dominant cyanobacteria genera during that period were Cylindrospermopsis, Planktolyngbya, Pseudanabaena and Microcystis. For ANA, all 17 reservoirs had concentrations below 0.1 μg L-1. Based on random forest analysis, the most important environmental factors affecting CYN concentrations were total nitrogen (most important), nitrate, total phosphorus and Cylindrospermopsis counts (least important). The findings of this study indicate that reducing total nitrogen concentrations may be useful in minimising CYN concentrations in tropical reservoirs.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/143618