Considerations for amino acid analysis by liquid chromatography-tandem mass spectrometry: A tutorial review

Violi, JP; Bishop, DP; Padula, MP; Steele, JR; Rodgers, KJ

Considerations for amino acid analysis by liquid chromatography-tandem mass spectrometry: A tutorial review

Violi, JP Bishop, DP Padula, MP Steele, JR Rodgers, KJ

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: TrAC - Trends in Analytical Chemistry, 2020, 131, pp. 116018-116018
Issue Date:: 2020-10-01

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Field	Value	Language
dc.contributor.author	Violi, JP
dc.contributor.author	Bishop, DP
dc.contributor.author	Padula, MP
dc.contributor.author	Steele, JR
dc.contributor.author	Rodgers, KJ
dc.date.accessioned	2020-11-01T22:03:49Z
dc.date.available	2020-11-01T22:03:49Z
dc.date.issued	2020-10-01
dc.identifier.citation	TrAC - Trends in Analytical Chemistry, 2020, 131, pp. 116018-116018
dc.identifier.issn	0165-9936
dc.identifier.issn	1879-3142
dc.identifier.uri	http://hdl.handle.net/10453/143679
dc.description.abstract	© 2020 Elsevier B.V. Amino acids are present in a variety of matrices from biological fluids to plant and animal tissues. We discuss options for amino acid extraction, sample clean-up and protein hydrolysis. Chromatographic separation of native amino acids is difficult due to their structure and physiochemical properties. Most analysts derivatise amino acids prior to analysis which improves the signal-to-noise ratio, provides more efficient ionisation and allows the use of reverse phase chromatography. Since chiral analysis is becoming increasingly important, we discuss chiral column chromatography and the use of chiral derivatisation agents. The choice of detector and parameters is based on a wide array of criteria including whether amino acids are native or derivatised and if targeted or untargeted analysis is being performed. This tutorial review aims to act as both a guide and to provide an overview of the techniques scientists currently employ for the analysis of amino acids via liquid chromatography-tandem mass spectrometry.
dc.language	en
dc.publisher	Elsevier BV
dc.relation	http://purl.org/au-research/grants/arc/DE180100194
dc.relation	Ian Potter Foundation
dc.relation.ispartof	TrAC - Trends in Analytical Chemistry
dc.relation.isbasedon	10.1016/j.trac.2020.116018
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	Considerations for amino acid analysis by liquid chromatography-tandem mass spectrometry: A tutorial review
dc.type	Journal Article
utslib.citation.volume	131
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CHT - Health Technologies
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Provost
pubs.organisational-group	/University of Technology Sydney/Provost/Jumbunna
utslib.copyright.status	open_access	*
utslib.copyright.embargo	2022-10-01T00:00:00+1000Z
dc.date.updated	2020-11-01T22:03:38Z
pubs.publication-status	Published
pubs.volume	131

Abstract:

© 2020 Elsevier B.V. Amino acids are present in a variety of matrices from biological fluids to plant and animal tissues. We discuss options for amino acid extraction, sample clean-up and protein hydrolysis. Chromatographic separation of native amino acids is difficult due to their structure and physiochemical properties. Most analysts derivatise amino acids prior to analysis which improves the signal-to-noise ratio, provides more efficient ionisation and allows the use of reverse phase chromatography. Since chiral analysis is becoming increasingly important, we discuss chiral column chromatography and the use of chiral derivatisation agents. The choice of detector and parameters is based on a wide array of criteria including whether amino acids are native or derivatised and if targeted or untargeted analysis is being performed. This tutorial review aims to act as both a guide and to provide an overview of the techniques scientists currently employ for the analysis of amino acids via liquid chromatography-tandem mass spectrometry.

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http://hdl.handle.net/10453/143679