Contribution of introns to the species diversity associated with the apicomplexan parasite, Neospora caninum.

Calarco, L; Ellis, J

Contribution of introns to the species diversity associated with the apicomplexan parasite, Neospora caninum.

Calarco, L

Ellis, J

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Publisher:: SPRINGER
Publication Type:: Journal Article
Citation:: Parasitology research, 2020, 119, (2), pp. 431-445
Issue Date:: 2020-02

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Field	Value	Language
dc.contributor.author	Calarco, L https://orcid.org/0000-0003-1911-6866
dc.contributor.author	Ellis, J https://orcid.org/0000-0001-7328-4831
dc.date.accessioned	2020-11-15T06:38:18Z
dc.date.available	2019-11-19
dc.date.available	2020-11-15T06:38:18Z
dc.date.issued	2020-02
dc.identifier.citation	Parasitology research, 2020, 119, (2), pp. 431-445
dc.identifier.issn	0932-0113
dc.identifier.issn	1432-1955
dc.identifier.uri	http://hdl.handle.net/10453/144020
dc.description.abstract	Neospora caninum is an intracellular parasite considered a leading cause of bovine reproduction failure worldwide, and a serious neurological disease of canines. Transplacental transmission in intermediate hosts is considered the most efficient means of transmission, which strictly involves asexual reproduction. Nonetheless, extensive genetic diversity has been reported within the species. What is yet to be elucidated are the major drivers of such diversity, and their impact on important parasite phenotypes such as virulence. Instead of protein-encoding sequences, genome and transcriptome data were used to investigate SNPs in introns between two distinct N. caninum isolates, with reported differences in pathogenicity. Variant analysis identified 840 and 501 SNPs within intergenic regions and introns, respectively, distinctly concentrated on chromosomes VI and XI, whereas the rest of the genome was monomorphic in comparison. Gene ontologies for SNP-dense intron-containing genes included ATP binding, transmembrane transport, protein kinase activity, and transcription and translation processes. This study shows that variation in non-coding DNA is contributing to N. caninum intraspecies genetic diversity, and potentially influencing and contributing to important parasite mechanisms. Finally, we present an assembled and annotated N. caninum apicoplast genome and show that this essential organelle is highly conserved between the two isolates, and related Coccidia.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	SPRINGER
dc.relation.ispartof	Parasitology research
dc.relation.isbasedon	10.1007/s00436-019-06561-x
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0605 Microbiology, 0707 Veterinary Sciences, 1108 Medical Microbiology
dc.subject.classification	Mycology & Parasitology
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Dogs
dc.subject.mesh	Neospora
dc.subject.mesh	Coccidiosis
dc.subject.mesh	Protozoan Proteins
dc.subject.mesh	Virulence
dc.subject.mesh	Amino Acid Sequence
dc.subject.mesh	Polymorphism, Single Nucleotide
dc.subject.mesh	Introns
dc.subject.mesh	Transcriptome
dc.subject.mesh	Whole Genome Sequencing
dc.subject.mesh	Amino Acid Sequence
dc.subject.mesh	Animals
dc.subject.mesh	Cattle
dc.subject.mesh	Coccidiosis
dc.subject.mesh	Dogs
dc.subject.mesh	Introns
dc.subject.mesh	Neospora
dc.subject.mesh	Polymorphism, Single Nucleotide
dc.subject.mesh	Protozoan Proteins
dc.subject.mesh	Transcriptome
dc.subject.mesh	Virulence
dc.subject.mesh	Whole Genome Sequencing
dc.title	Contribution of introns to the species diversity associated with the apicomplexan parasite, Neospora caninum.
dc.type	Journal Article
utslib.citation.volume	119
utslib.location.activity	Germany
utslib.for	0605 Microbiology
utslib.for	0707 Veterinary Sciences
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2020-11-15T06:38:12Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	119
utslib.citation.issue	2

Abstract:

Neospora caninum is an intracellular parasite considered a leading cause of bovine reproduction failure worldwide, and a serious neurological disease of canines. Transplacental transmission in intermediate hosts is considered the most efficient means of transmission, which strictly involves asexual reproduction. Nonetheless, extensive genetic diversity has been reported within the species. What is yet to be elucidated are the major drivers of such diversity, and their impact on important parasite phenotypes such as virulence. Instead of protein-encoding sequences, genome and transcriptome data were used to investigate SNPs in introns between two distinct N. caninum isolates, with reported differences in pathogenicity. Variant analysis identified 840 and 501 SNPs within intergenic regions and introns, respectively, distinctly concentrated on chromosomes VI and XI, whereas the rest of the genome was monomorphic in comparison. Gene ontologies for SNP-dense intron-containing genes included ATP binding, transmembrane transport, protein kinase activity, and transcription and translation processes. This study shows that variation in non-coding DNA is contributing to N. caninum intraspecies genetic diversity, and potentially influencing and contributing to important parasite mechanisms. Finally, we present an assembled and annotated N. caninum apicoplast genome and show that this essential organelle is highly conserved between the two isolates, and related Coccidia.

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http://hdl.handle.net/10453/144020