Draft genomes of Perkinsus olseni and Perkinsus chesapeaki reveal polyploidy and regional differences in heterozygosity.

Bogema, DR; Yam, J; Micallef, ML; Gholipourkanani, H; Go, J; Jenkins, C; Dang, C

Draft genomes of Perkinsus olseni and Perkinsus chesapeaki reveal polyploidy and regional differences in heterozygosity.

Bogema, DR Yam, J Micallef, ML Gholipourkanani, H Go, J Jenkins, C Dang, C

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Publisher:: Elsevier BV
Publication Type:: Journal Article
Citation:: Genomics, 2020
Issue Date:: 2020-10-02

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Field	Value	Language
dc.contributor.author	Bogema, DR
dc.contributor.author	Yam, J
dc.contributor.author	Micallef, ML
dc.contributor.author	Gholipourkanani, H
dc.contributor.author	Go, J
dc.contributor.author	Jenkins, C
dc.contributor.author	Dang, C
dc.date.accessioned	2020-11-20T00:56:37Z
dc.date.available	2020-09-30
dc.date.available	2020-11-20T00:56:37Z
dc.date.issued	2020-10-02
dc.identifier.citation	Genomics, 2020
dc.identifier.issn	0888-7543
dc.identifier.issn	1089-8646
dc.identifier.uri	http://hdl.handle.net/10453/144170
dc.description.abstract	Perkinsus spp. parasites have significant impact on aquaculture and wild mollusc populations. We sequenced the genomes of five monoclonal isolates of Perkinsus olseni and one Perkinsus chesapeaki from international sources. Sequence analysis revealed similar levels of repetitive sequence within species, a polyploid genome structure, and substantially higher heterozygosity in Oceanian-sourced isolates. We also identified tandem replication of the rRNA transcriptional unit, with high strain variation. Characterized gene content was broadly similar amongst all Perkinsus spp. but P. olseni Oceanian isolates contained an elevated number of genes compared to other P. olseni isolates and cox3 could not be identified in any Perkinsus spp. sequence. Phylogenetics and average nucleotide identity scans were consistent with all P. olseni isolates being within one species. These are the first genome sequences generated for both P. olseni and P. chesapeaki and will allow future advances in diagnostic design and population genomics of these important aquatic parasites.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier BV
dc.relation.ispartof	Genomics
dc.relation.isbasedon	10.1016/j.ygeno.2020.09.064
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0604 Genetics, 0806 Information Systems
dc.subject.classification	Genetics & Heredity
dc.title	Draft genomes of Perkinsus olseni and Perkinsus chesapeaki reveal polyploidy and regional differences in heterozygosity.
dc.type	Journal Article
utslib.location.activity	United States
utslib.for	0604 Genetics
utslib.for	0806 Information Systems
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-11-20T00:56:29Z
pubs.publication-status	Published

Abstract:

Perkinsus spp. parasites have significant impact on aquaculture and wild mollusc populations. We sequenced the genomes of five monoclonal isolates of Perkinsus olseni and one Perkinsus chesapeaki from international sources. Sequence analysis revealed similar levels of repetitive sequence within species, a polyploid genome structure, and substantially higher heterozygosity in Oceanian-sourced isolates. We also identified tandem replication of the rRNA transcriptional unit, with high strain variation. Characterized gene content was broadly similar amongst all Perkinsus spp. but P. olseni Oceanian isolates contained an elevated number of genes compared to other P. olseni isolates and cox3 could not be identified in any Perkinsus spp. sequence. Phylogenetics and average nucleotide identity scans were consistent with all P. olseni isolates being within one species. These are the first genome sequences generated for both P. olseni and P. chesapeaki and will allow future advances in diagnostic design and population genomics of these important aquatic parasites.

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http://hdl.handle.net/10453/144170