Unlocking the phylogenetic diversity, primary habitats, and abundances of free-living Symbiodiniaceae on a coral reef.

Fujise, L; Suggett, DJ; Stat, M; Kahlke, T; Bunce, M; Gardner, SG; Goyen, S; Woodcock, S; Ralph, PJ; Seymour, JR; Siboni, N; Nitschke, MR

Unlocking the phylogenetic diversity, primary habitats, and abundances of free-living Symbiodiniaceae on a coral reef.

Fujise, L Suggett, DJ Stat, M Kahlke, T

Bunce, M Gardner, SG Goyen, S

Woodcock, S

Ralph, PJ Seymour, JR Siboni, N

Nitschke, MR

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Molecular ecology, 2020
Issue Date:: 2020-11-03

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Field	Value	Language
dc.contributor.author	Fujise, L
dc.contributor.author	Suggett, DJ
dc.contributor.author	Stat, M
dc.contributor.author	Kahlke, T https://orcid.org/0000-0002-9762-6573
dc.contributor.author	Bunce, M
dc.contributor.author	Gardner, SG
dc.contributor.author	Goyen, S https://orcid.org/0000-0003-3691-4640
dc.contributor.author	Woodcock, S https://orcid.org/0000-0003-4903-3164
dc.contributor.author	Ralph, PJ
dc.contributor.author	Seymour, JR
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949
dc.contributor.author	Nitschke, MR
dc.date.accessioned	2020-11-23T04:15:57Z
dc.date.available	2020-11-23T04:15:57Z
dc.date.issued	2020-11-03
dc.identifier.citation	Molecular ecology, 2020
dc.identifier.issn	0962-1083
dc.identifier.issn	1365-294X
dc.identifier.uri	http://hdl.handle.net/10453/144250
dc.description.abstract	Dinoflagellates of the family Symbiodiniaceae form mutualistic symbioses with marine invertebrates such as reef-building corals, but also inhabit reef environments as free-living cells. Most coral species acquire Symbiodiniaceae horizontally from the surrounding environment during the larval and/or recruitment phase, however the phylogenetic diversity and ecology of free-living Symbiodiniaceae on coral reefs is largely unknown. We coupled environmental DNA sequencing and genus-specific qPCR to resolve the community structure and cell abundances of free-living Symbiodiniaceae in the water column, sediment, and macroalgae and compared these to coral symbionts. Sampling was conducted at two time points, one of which coincided with the annual coral spawning event when recombination between hosts and free-living Symbiodiniaceae is assumed to be critical. Amplicons of the internal transcribed spacer (ITS2) region were assigned to 12 of the 15 Symbiodiniaceae genera or genera-equivalent lineages. Community compositions were separated by habitat, with water samples containing a high proportion of sequences corresponding to coral symbionts of the genus Cladocopium, potentially as a result of cell expulsion from in hospite populations. Sediment-associated Symbiodiniaceae communities were distinct, potentially due to the presence of exclusively free-living species. Intriguingly, macroalgal surfaces displayed the highest cell abundances of Symbiodiniaceae, suggesting a key role for macroalgae in ensuring the ecological success of corals through maintenance of a continuum between environmental and symbiotic populations of Symbiodiniaceae.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation	http://purl.org/au-research/grants/arc/DP160100271
dc.relation.ispartof	Molecular ecology
dc.relation.isbasedon	10.1111/mec.15719
dc.rights	info:eu-repo/semantics/openAccess
dc.rights	This is the peer reviewed version of the following article: Unlocking the phylogenetic diversity, primary habitats, and abundances of free-living Symbiodiniaceae on a coral reef. Molecular ecology First published: 03 Nov 2020 which has been published in final form at https://onlinelibrary.wiley.com/doi/10.1111/mec.15719 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving."
dc.subject	06 Biological Sciences
dc.subject.classification	Evolutionary Biology
dc.title	Unlocking the phylogenetic diversity, primary habitats, and abundances of free-living Symbiodiniaceae on a coral reef.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	06 Biological Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2020-11-23T04:15:37Z
pubs.publication-status	Published

Abstract:

Dinoflagellates of the family Symbiodiniaceae form mutualistic symbioses with marine invertebrates such as reef-building corals, but also inhabit reef environments as free-living cells. Most coral species acquire Symbiodiniaceae horizontally from the surrounding environment during the larval and/or recruitment phase, however the phylogenetic diversity and ecology of free-living Symbiodiniaceae on coral reefs is largely unknown. We coupled environmental DNA sequencing and genus-specific qPCR to resolve the community structure and cell abundances of free-living Symbiodiniaceae in the water column, sediment, and macroalgae and compared these to coral symbionts. Sampling was conducted at two time points, one of which coincided with the annual coral spawning event when recombination between hosts and free-living Symbiodiniaceae is assumed to be critical. Amplicons of the internal transcribed spacer (ITS2) region were assigned to 12 of the 15 Symbiodiniaceae genera or genera-equivalent lineages. Community compositions were separated by habitat, with water samples containing a high proportion of sequences corresponding to coral symbionts of the genus Cladocopium, potentially as a result of cell expulsion from in hospite populations. Sediment-associated Symbiodiniaceae communities were distinct, potentially due to the presence of exclusively free-living species. Intriguingly, macroalgal surfaces displayed the highest cell abundances of Symbiodiniaceae, suggesting a key role for macroalgae in ensuring the ecological success of corals through maintenance of a continuum between environmental and symbiotic populations of Symbiodiniaceae.

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http://hdl.handle.net/10453/144250