In vitro and ex vivo strategies for intracellular delivery.

Stewart, MP; Sharei, A; Ding, X; Sahay, G; Langer, R; Jensen, KF

In vitro and ex vivo strategies for intracellular delivery.

Stewart, MP Sharei, A Ding, X Sahay, G Langer, R Jensen, KF

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Nature, 2016, 538, (7624), pp. 183-192
Issue Date:: 2016-10

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Field	Value	Language
dc.contributor.author	Stewart, MP
dc.contributor.author	Sharei, A
dc.contributor.author	Ding, X
dc.contributor.author	Sahay, G
dc.contributor.author	Langer, R
dc.contributor.author	Jensen, KF
dc.date.accessioned	2020-11-25T07:43:57Z
dc.date.available	2016-07-11
dc.date.available	2020-11-25T07:43:57Z
dc.date.issued	2016-10
dc.identifier.citation	Nature, 2016, 538, (7624), pp. 183-192
dc.identifier.issn	0028-0836
dc.identifier.issn	1476-4687
dc.identifier.uri	http://hdl.handle.net/10453/144345
dc.description.abstract	Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.
dc.format	Print
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Nature
dc.relation.isbasedon	10.1038/nature19764
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	General Science & Technology
dc.subject.mesh	Cell Membrane
dc.subject.mesh	Intracellular Space
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Drug Delivery Systems
dc.subject.mesh	Transfection
dc.subject.mesh	Microfluidics
dc.subject.mesh	Nanotechnology
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Animals
dc.subject.mesh	Cell Membrane
dc.subject.mesh	Drug Delivery Systems
dc.subject.mesh	Humans
dc.subject.mesh	In Vitro Techniques
dc.subject.mesh	Intracellular Space
dc.subject.mesh	Lab-On-A-Chip Devices
dc.subject.mesh	Microfluidics
dc.subject.mesh	Nanotechnology
dc.subject.mesh	Transfection
dc.title	In vitro and ex vivo strategies for intracellular delivery.
dc.type	Journal Article
utslib.citation.volume	538
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-11-25T07:43:54Z
pubs.issue	7624
pubs.publication-status	Published
pubs.volume	538
utslib.citation.issue	7624

Abstract:

Intracellular delivery of materials has become a critical component of genome-editing approaches, ex vivo cell-based therapies, and a diversity of fundamental research applications. Limitations of current technologies motivate development of next-generation systems that can deliver a broad variety of cargo to diverse cell types. Here we review in vitro and ex vivo intracellular delivery approaches with a focus on mechanisms, challenges and opportunities. In particular, we emphasize membrane-disruption-based delivery methods and the transformative role of nanotechnology, microfluidics and laboratory-on-chip technology in advancing the field.

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http://hdl.handle.net/10453/144345