Finding the proverbial needle: Non-targeted screening of synthetic opioids in equine plasma.

Klingberg, J; Cawley, A; Shimmon, R; Fouracre, C; Pasin, D; Fu, S

Finding the proverbial needle: Non-targeted screening of synthetic opioids in equine plasma.

Klingberg, J Cawley, A Shimmon, R

Fouracre, C Pasin, D

Fu, S

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Drug testing and analysis, 2020
Issue Date:: 2020-07-05

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Field	Value	Language
dc.contributor.author	Klingberg, J
dc.contributor.author	Cawley, A
dc.contributor.author	Shimmon, R https://orcid.org/0000-0002-8763-991X
dc.contributor.author	Fouracre, C
dc.contributor.author	Pasin, D https://orcid.org/0000-0002-5037-7290
dc.contributor.author	Fu, S https://orcid.org/0000-0002-6238-3612
dc.date.accessioned	2020-11-26T05:05:04Z
dc.date.available	2021-08-12T19:00:48Z
dc.date.issued	2020-07-05
dc.identifier.citation	Drug testing and analysis, 2020
dc.identifier.issn	1942-7603
dc.identifier.issn	1942-7611
dc.identifier.uri	http://hdl.handle.net/10453/144382
dc.description.abstract	Synthetic opioids are a class of compounds that are of particular concern due to their high potency and potential health impacts. With the relentless emergence of new synthetic opioid derivatives, non-targeted screening strategies are required that do not rely on the use of library spectra or reference materials. In this study, product ion searching, and Kendrick mass defect analysis were investigated for non-targeted screening of synthetic opioids. The estimated screening cut-offs for these techniques ranged between 0.05 and 0.1 ng/mL. These techniques were designed to not be reliant on a particular vendor's software, meaning that they can be applied to existing drug screening protocols, without requiring the development and validation of new analytical procedures. The efficacy of the developed techniques was tested through blind trials, with spiked samples inserted amongst authentic plasma samples, which demonstrated the usefulness of these methods for high-throughput screening. The use of a non-targeted screening workflow that contains complementary techniques can increase the likelihood of detecting compounds of interest within a sample, as well as the confidence in detections that are made.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	Drug testing and analysis
dc.relation.isbasedon	10.1002/dta.2893
dc.rights	info:eu-repo/semantics/embargoedAccess
dc.subject	0301 Analytical Chemistry, 0601 Biochemistry and Cell Biology, 1115 Pharmacology and Pharmaceutical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	Finding the proverbial needle: Non-targeted screening of synthetic opioids in equine plasma.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	0301 Analytical Chemistry
utslib.for	0601 Biochemistry and Cell Biology
utslib.for	1115 Pharmacology and Pharmaceutical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - CFS - Centre for Forensic Science
pubs.organisational-group	/University of Technology Sydney/Strength - CCET - Centre for Clean Energy Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-11-26T05:04:53Z
pubs.publication-status	Published

Abstract:

Synthetic opioids are a class of compounds that are of particular concern due to their high potency and potential health impacts. With the relentless emergence of new synthetic opioid derivatives, non-targeted screening strategies are required that do not rely on the use of library spectra or reference materials. In this study, product ion searching, and Kendrick mass defect analysis were investigated for non-targeted screening of synthetic opioids. The estimated screening cut-offs for these techniques ranged between 0.05 and 0.1 ng/mL. These techniques were designed to not be reliant on a particular vendor's software, meaning that they can be applied to existing drug screening protocols, without requiring the development and validation of new analytical procedures. The efficacy of the developed techniques was tested through blind trials, with spiked samples inserted amongst authentic plasma samples, which demonstrated the usefulness of these methods for high-throughput screening. The use of a non-targeted screening workflow that contains complementary techniques can increase the likelihood of detecting compounds of interest within a sample, as well as the confidence in detections that are made.

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http://hdl.handle.net/10453/144382