Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential.

Johnson, L; Waters, L; Green, S; Wood, B; Marks, DC

Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential.

Johnson, L

Waters, L

Green, S Wood, B Marks, DC

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Publisher:: Wiley
Publication Type:: Journal Article
Citation:: Transfusion, 2020, 60, (3), pp. 454-459
Issue Date:: 2020

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Field	Value	Language
dc.contributor.author	Johnson, L https://orcid.org/0000-0003-3303-4437
dc.contributor.author	Waters, L https://orcid.org/0000-0002-4740-055X
dc.contributor.author	Green, S
dc.contributor.author	Wood, B
dc.contributor.author	Marks, DC
dc.date.accessioned	2020-11-27T02:12:42Z
dc.date.available	2019-11-12
dc.date.available	2020-11-27T02:12:42Z
dc.date.issued	2020
dc.identifier.citation	Transfusion, 2020, 60, (3), pp. 454-459
dc.identifier.issn	0041-1132
dc.identifier.issn	1537-2995
dc.identifier.uri	http://hdl.handle.net/10453/144398
dc.description.abstract	BACKGROUND AND OBJECTIVES:Cryopreservation provides an option for long-term storage of platelet concentrates. While platelets are usually frozen as soon as practical after collection (within 2 days), the ability to freeze units at a later stage of the shelf life may improve inventory management. As such, the aim of this study was to determine the impact of freezing platelets approaching expiry (Day 5/6). MATERIALS AND METHODS:Two ABO-matched buffy coat-derived platelets (30% plasma/70% platelet additive solution) were pooled and split to produce matched pairs (n = 8 pairs). Platelets were frozen on Day 1 after collection (cryopreserved platelets [CPPs]) or Day 5 or 6 (expired-CPPs) at -80°C with 5% to 6% dimethyl sulfoxide. In vitro platelet quality was tested before freezing and after thawing and reconstitution in plasma. RESULTS:The majority of prefreeze parameters were equivalent for all platelet units (Day 1 vs. Day 5 or 6). Expired-CPPs had a higher mean postthaw platelet recovery (82 ± 4%) compared to CPPs (75 ± 4%; p = 0.0021). Cryopreservation resulted in a loss of surface glycoproteins (glycoprotein (GP) Ibα, GPIIb, GPVI), an increase in activation markers (phosphatidylserine and P-selectin) and microparticle release, compared to unfrozen platelets. However, the cryopreservation-induced changes were equivalent in CPPs and expired-CPPs. Functionality was measured by thromboelastography and was similar between expired-CPPs (R-time: 5.3 ± 0.3) and CPPs (R-time: 5.4 ± 0.5; p = 0.7094). CONCLUSION:The phenotype and functional profile of platelets frozen at expiry were similar to platelets frozen 1 day following collection. These data suggest that expired platelets may represent a suitable starting material for cryopreservation.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Wiley
dc.relation.ispartof	Transfusion
dc.relation.isbasedon	10.1111/trf.15616
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	1102 Cardiorespiratory Medicine and Haematology, 1103 Clinical Sciences, 1107 Immunology
dc.subject.classification	Cardiovascular System & Hematology
dc.subject.mesh	Blood Platelets
dc.subject.mesh	Humans
dc.subject.mesh	Cryopreservation
dc.subject.mesh	Freezing
dc.subject.mesh	Blood Buffy Coat
dc.subject.mesh	Blood Buffy Coat
dc.subject.mesh	Blood Platelets
dc.subject.mesh	Cryopreservation
dc.subject.mesh	Freezing
dc.subject.mesh	Humans
dc.title	Freezing expired platelets does not compromise in vitro quality: An opportunity to maximize inventory potential.
dc.type	Journal Article
utslib.citation.volume	60
utslib.location.activity	United States
utslib.for	1103 Clinical Sciences
utslib.for	1102 Cardiorespiratory Medicine and Haematology
utslib.for	1107 Immunology
utslib.for	1102 Cardiorespiratory Medicine and Haematology
utslib.for	1103 Clinical Sciences
utslib.for	1107 Immunology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	true
dc.date.updated	2020-11-27T02:12:38Z
pubs.issue	3
pubs.publication-status	Published online
pubs.volume	60
utslib.citation.issue	3

Abstract:

BACKGROUND AND OBJECTIVES:Cryopreservation provides an option for long-term storage of platelet concentrates. While platelets are usually frozen as soon as practical after collection (within 2 days), the ability to freeze units at a later stage of the shelf life may improve inventory management. As such, the aim of this study was to determine the impact of freezing platelets approaching expiry (Day 5/6). MATERIALS AND METHODS:Two ABO-matched buffy coat-derived platelets (30% plasma/70% platelet additive solution) were pooled and split to produce matched pairs (n = 8 pairs). Platelets were frozen on Day 1 after collection (cryopreserved platelets [CPPs]) or Day 5 or 6 (expired-CPPs) at -80°C with 5% to 6% dimethyl sulfoxide. In vitro platelet quality was tested before freezing and after thawing and reconstitution in plasma. RESULTS:The majority of prefreeze parameters were equivalent for all platelet units (Day 1 vs. Day 5 or 6). Expired-CPPs had a higher mean postthaw platelet recovery (82 ± 4%) compared to CPPs (75 ± 4%; p = 0.0021). Cryopreservation resulted in a loss of surface glycoproteins (glycoprotein (GP) Ibα, GPIIb, GPVI), an increase in activation markers (phosphatidylserine and P-selectin) and microparticle release, compared to unfrozen platelets. However, the cryopreservation-induced changes were equivalent in CPPs and expired-CPPs. Functionality was measured by thromboelastography and was similar between expired-CPPs (R-time: 5.3 ± 0.3) and CPPs (R-time: 5.4 ± 0.5; p = 0.7094). CONCLUSION:The phenotype and functional profile of platelets frozen at expiry were similar to platelets frozen 1 day following collection. These data suggest that expired platelets may represent a suitable starting material for cryopreservation.

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http://hdl.handle.net/10453/144398