An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy

Söderström, B; Ruda, A; Widmalm, G; Daley, DO

An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy

Söderström, B Ruda, A Widmalm, G Daley, DO

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Publisher:: Microbiology Society
Publication Type:: Journal Article
Citation:: Microbiology, 2020
Issue Date:: 2020-11-25

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Field	Value	Language
dc.contributor.author	Söderström, B
dc.contributor.author	Ruda, A
dc.contributor.author	Widmalm, G
dc.contributor.author	Daley, DO
dc.date.accessioned	2020-12-03T05:18:37Z
dc.date.available	2020-12-03T05:18:37Z
dc.date.issued	2020-11-25
dc.identifier.citation	Microbiology, 2020
dc.identifier.issn	1350-0872
dc.identifier.issn	1465-2080
dc.identifier.uri	http://hdl.handle.net/10453/144486
dc.description.abstract	<jats:p>Fluorescent <jats:sc>d</jats:sc>-amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an OregonGreen488-labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Gram-positive and some Gram-negative bacteria, and imaged by super-resolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm.</jats:p>
dc.language	en
dc.publisher	Microbiology Society
dc.relation.ispartof	Microbiology
dc.relation.isbasedon	10.1099/mic.0.000996
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.classification	Microbiology
dc.title	An OregonGreen488-labelled d-amino acid for visualizing peptidoglycan by super-resolution STED nanoscopy
dc.type	Journal Article
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-12-03T05:18:28Z
pubs.publication-status	Published

Abstract:

Fluorescent d-amino acids (FDAAs) are molecular probes that are widely used for labelling the peptidoglycan layer of bacteria. When added to growing cells they are incorporated into the stem peptide by a transpeptidase reaction, allowing the timing and localization of peptidoglycan synthesis to be determined by fluorescence microscopy. Herein we describe the chemical synthesis of an OregonGreen488-labelled FDAA (OGDA). We also demonstrate that OGDA can be efficiently incorporated into the PG of Gram-positive and some Gram-negative bacteria, and imaged by super-resolution stimulated emission depletion (STED) nanoscopy at a resolution well below 100 nm.

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http://hdl.handle.net/10453/144486