COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins.

Woldhuis, RR; Heijink, IH; van den Berge, M; Timens, W; Oliver, BGG; de Vries, M; Brandsma, C-A

COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins.

Woldhuis, RR Heijink, IH van den Berge, M Timens, W Oliver, BGG de Vries, M Brandsma, C-A

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Publisher:: BMJ
Publication Type:: Journal Article
Citation:: Thorax, 2020, pp. thoraxjnl-2020-215114
Issue Date:: 2020-12-03

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Field	Value	Language
dc.contributor.author	Woldhuis, RR
dc.contributor.author	Heijink, IH
dc.contributor.author	van den Berge, M
dc.contributor.author	Timens, W
dc.contributor.author	Oliver, BGG
dc.contributor.author	de Vries, M
dc.contributor.author	Brandsma, C-A
dc.date.accessioned	2020-12-14T03:59:47Z
dc.date.available	2020-10-22
dc.date.available	2020-12-14T03:59:47Z
dc.date.issued	2020-12-03
dc.identifier.citation	Thorax, 2020, pp. thoraxjnl-2020-215114
dc.identifier.issn	0040-6376
dc.identifier.issn	1468-3296
dc.identifier.uri	http://hdl.handle.net/10453/144674
dc.description.abstract	COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (SEO)-COPD. SASP protein secretion was measured after paraquat-induced senescence in lung fibroblasts using Olink Proteomics and compared between (SEO-)COPD-derived and control-derived fibroblasts. We identified 124 SASP proteins of senescent lung fibroblasts, of which 42 were secreted at higher levels by COPD-derived fibroblasts and 35 by SEO-COPD-derived fibroblasts compared with controls. Interestingly, the (SEO-)COPD-associated SASP included proteins involved in chronic inflammation, which may contribute to (SEO-)COPD pathogenesis.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	BMJ
dc.relation.ispartof	Thorax
dc.relation.isbasedon	10.1136/thoraxjnl-2020-215114
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1103 Clinical Sciences
dc.subject.classification	Respiratory System
dc.title	COPD-derived fibroblasts secrete higher levels of senescence-associated secretory phenotype proteins.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	1103 Clinical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
dc.date.updated	2020-12-14T03:59:42Z
pubs.publication-status	Published

Abstract:

COPD-derived fibroblasts have increased cellular senescence. Senescent cell accumulation can induce tissue dysfunction by their senescence-associated secretory phenotype (SASP). We aimed to determine the SASP of senescent fibroblasts and COPD-derived lung fibroblasts, including severe, early-onset (SEO)-COPD. SASP protein secretion was measured after paraquat-induced senescence in lung fibroblasts using Olink Proteomics and compared between (SEO-)COPD-derived and control-derived fibroblasts. We identified 124 SASP proteins of senescent lung fibroblasts, of which 42 were secreted at higher levels by COPD-derived fibroblasts and 35 by SEO-COPD-derived fibroblasts compared with controls. Interestingly, the (SEO-)COPD-associated SASP included proteins involved in chronic inflammation, which may contribute to (SEO-)COPD pathogenesis.

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http://hdl.handle.net/10453/144674