Global distribution of a chlorophyll f cyanobacterial marker.

Antonaru, LA; Cardona, T; Larkum, AWD; Nürnberg, DJ

Global distribution of a chlorophyll f cyanobacterial marker.

Antonaru, LA Cardona, T Larkum, AWD Nürnberg, DJ

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: The ISME journal, 2020, 14, (9), pp. 2275-2287
Issue Date:: 2020-09

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Field	Value	Language
dc.contributor.author	Antonaru, LA
dc.contributor.author	Cardona, T
dc.contributor.author	Larkum, AWD
dc.contributor.author	Nürnberg, DJ
dc.date.accessioned	2020-12-14T05:02:43Z
dc.date.available	2020-04-28
dc.date.available	2020-12-14T05:02:43Z
dc.date.issued	2020-09
dc.identifier.citation	The ISME journal, 2020, 14, (9), pp. 2275-2287
dc.identifier.issn	1751-7362
dc.identifier.issn	1751-7370
dc.identifier.uri	http://hdl.handle.net/10453/144678
dc.description.abstract	Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype's distribution.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	The ISME journal
dc.relation.isbasedon	10.1038/s41396-020-0670-y
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Microbiology
dc.title	Global distribution of a chlorophyll f cyanobacterial marker.
dc.type	Journal Article
utslib.citation.volume	14
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-12-14T05:02:29Z
pubs.issue	9
pubs.publication-status	Published
pubs.volume	14
utslib.citation.issue	9

Abstract:

Some cyanobacteria use light outside the visible spectrum for oxygenic photosynthesis. The far-red light (FRL) region is made accessible through a complex acclimation process that involves the formation of new phycobilisomes and photosystems containing chlorophyll f. Diverse cyanobacteria ranging from unicellular to branched-filamentous forms show this response. These organisms have been isolated from shaded environments such as microbial mats, soil, rock, and stromatolites. However, the full spread of chlorophyll f-containing species in nature is still unknown. Currently, discovering new chlorophyll f cyanobacteria involves lengthy incubation times under selective far-red light. We have used a marker gene to detect chlorophyll f organisms in environmental samples and metagenomic data. This marker, apcE2, encodes a phycobilisome linker associated with FRL-photosynthesis. By focusing on a far-red motif within the sequence, degenerate PCR and BLAST searches can effectively discriminate against the normal chlorophyll a-associated apcE. Even short recovered sequences carry enough information for phylogenetic placement. Markers of chlorophyll f photosynthesis were found in metagenomic datasets from diverse environments around the globe, including cyanobacterial symbionts, hypersaline lakes, corals, and the Arctic/Antarctic regions. This additional information enabled higher phylogenetic resolution supporting the hypothesis that vertical descent, as opposed to horizontal gene transfer, is largely responsible for this phenotype's distribution.

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http://hdl.handle.net/10453/144678