| Field |
Value |
Language |
|
dc.contributor.author |
Zhanghao, K |
|
|
dc.contributor.author |
Liu, W |
|
|
dc.contributor.author |
Li, M |
|
|
dc.contributor.author |
Wu, Z |
|
|
dc.contributor.author |
Wang, X |
|
|
dc.contributor.author |
Chen, X |
|
|
dc.contributor.author |
Shan, C |
|
|
dc.contributor.author |
Wang, H |
|
|
dc.contributor.author |
Chen, X |
|
|
dc.contributor.author |
Dai, Q |
|
|
dc.contributor.author |
Xi, P |
|
|
dc.contributor.author |
Jin, D
https://orcid.org/0000-0003-1046-2666
|
|
|
dc.date.accessioned |
2020-12-15T01:23:42Z |
|
|
dc.date.available |
2020-10-29 |
|
|
dc.date.available |
2020-12-15T01:23:42Z |
|
|
dc.date.issued |
2020-11-18 |
|
|
dc.identifier.citation |
Nature communications, 2020, 11, (1) |
|
|
dc.identifier.issn |
2041-1723 |
|
|
dc.identifier.issn |
2041-1723 |
|
|
dc.identifier.uri |
http://hdl.handle.net/10453/144697
|
|
|
dc.description.abstract |
Lipid membranes are found in most intracellular organelles, and their heterogeneities play an essential role in regulating the organelles' biochemical functionalities. Here we report a Spectrum and Polarization Optical Tomography (SPOT) technique to study the subcellular lipidomics in live cells. Simply using one dye that universally stains the lipid membranes, SPOT can simultaneously resolve the membrane morphology, polarity, and phase from the three optical-dimensions of intensity, spectrum, and polarization, respectively. These high-throughput optical properties reveal lipid heterogeneities of ten subcellular compartments, at different developmental stages, and even within the same organelle. Furthermore, we obtain real-time monitoring of the multi-organelle interactive activities of cell division and successfully reveal their sophisticated lipid dynamics during the plasma membrane separation, tunneling nanotubules formation, and mitochondrial cristae dissociation. This work suggests research frontiers in correlating single-cell super-resolution lipidomics with multiplexed imaging of organelle interactome. |
|
|
dc.format |
Electronic |
|
|
dc.language |
eng |
|
|
dc.publisher |
Springer Science and Business Media LLC |
|
|
dc.relation.ispartof |
Nature communications |
|
|
dc.relation.isbasedon |
10.1038/s41467-020-19747-0 |
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|
dc.rights |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
|
|
dc.rights |
info:eu-repo/semantics/openAccess |
|
|
dc.rights |
This is a post-peer-review, pre-copyedit version of an article published in Nature communications. The final authenticated version is available online at: https://dx.doi.org/10.1038/s41467-020-19747-0. |
en_US |
|
dc.rights |
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
|
|
dc.title |
High-dimensional super-resolution imaging reveals heterogeneity and dynamics of subcellular lipid membranes. |
|
|
dc.type |
Journal Article |
|
|
utslib.citation.volume |
11 |
|
|
utslib.location.activity |
England |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science |
|
|
pubs.organisational-group |
/University of Technology Sydney/Strength - IBMD - Initiative for Biomedical Devices |
|
|
pubs.organisational-group |
/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences |
|
|
pubs.organisational-group |
/University of Technology Sydney |
|
|
utslib.copyright.status |
open_access |
* |
|
pubs.consider-herdc |
false |
|
|
dc.date.updated |
2020-12-15T01:23:31Z |
|
|
pubs.issue |
1 |
|
|
pubs.publication-status |
Published |
|
|
pubs.volume |
11 |
|
|
utslib.citation.issue |
1 |
|
