Highly heterogeneous temporal dynamics in the abundance and diversity of the emerging pathogens Arcobacter at an urban beach.

Carney, RL; Brown, MV; Siboni, N; Raina, J-B; Kahlke, T; Mitrovic, SM; Seymour, JR

Highly heterogeneous temporal dynamics in the abundance and diversity of the emerging pathogens Arcobacter at an urban beach.

Carney, RL Brown, MV Siboni, N

Raina, J-B Kahlke, T

Mitrovic, SM Seymour, JR

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Publisher:: PERGAMON-ELSEVIER SCIENCE LTD
Publication Type:: Journal Article
Citation:: Water research, 2020, 171
Issue Date:: 2020-03

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Field	Value	Language
dc.contributor.author	Carney, RL
dc.contributor.author	Brown, MV
dc.contributor.author	Siboni, N https://orcid.org/0000-0001-6082-0949
dc.contributor.author	Raina, J-B
dc.contributor.author	Kahlke, T https://orcid.org/0000-0002-9762-6573
dc.contributor.author	Mitrovic, SM
dc.contributor.author	Seymour, JR
dc.date.accessioned	2020-12-15T03:05:55Z
dc.date.available	2019-12-16
dc.date.available	2020-12-15T03:05:55Z
dc.date.issued	2020-03
dc.identifier.citation	Water research, 2020, 171
dc.identifier.issn	0043-1354
dc.identifier.issn	1879-2448
dc.identifier.uri	http://hdl.handle.net/10453/144705
dc.description.abstract	While the significance of Arcobacter in clinical settings grows, the ecological dynamics of potentially pathogenic Arcobacter in coastal marine environments remains unclear. In this study, we monitored the temporal dynamics of Arcobacter at an urban beach subject to significant stormwater input and wet weather sewer overflows (WWSO). Weekly monitoring of bacterial communities over 24 months using 16S rRNA amplicon sequencing revealed large, intermittent peaks in the relative abundance of Arcobacter. Quantitative PCR was subsequently employed to track absolute abundance of Arcobacter 23S rRNA gene copies, revealing peaks in abundance reaching up to 108 gene copies L-1, with these increases statistically correlated with stormwater and WWSO intrusion. Notably, peaks in Arcobacter abundance were poorly correlated with enterococci plate counts, and remained elevated for one week following heavy rainfall. Using oligotyping we discriminated single nucleotide variants (SNVs) within the Arcobacter population, revealing 10 distinct clusters of SNVs that we defined as Arcobacter "ecotypes", with each displaying distinct temporal dynamics. The most abundant ecotype during stormwater and modelled WWSO events displayed 16S rRNA sequence similarity to A. cryaerophilius, a species previously implicated in human illness. Our findings highlight the diverse environmental drivers of Arcobacter abundance within coastal settings and point to a potentially important, yet overlooked exposure risk of these potential pathogens to humans.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	PERGAMON-ELSEVIER SCIENCE LTD
dc.relation	http://purl.org/au-research/grants/arc/FT130100218
dc.relation.ispartof	Water research
dc.relation.isbasedon	10.1016/j.watres.2019.115405
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject.classification	Environmental Engineering
dc.subject.mesh	Humans
dc.subject.mesh	Bacteria
dc.subject.mesh	Arcobacter
dc.subject.mesh	DNA, Bacterial
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Real-Time Polymerase Chain Reaction
dc.subject.mesh	Humans
dc.subject.mesh	Bacteria
dc.subject.mesh	Arcobacter
dc.subject.mesh	DNA, Bacterial
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Real-Time Polymerase Chain Reaction
dc.subject.mesh	Arcobacter
dc.subject.mesh	Bacteria
dc.subject.mesh	DNA, Bacterial
dc.subject.mesh	Humans
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Real-Time Polymerase Chain Reaction
dc.title	Highly heterogeneous temporal dynamics in the abundance and diversity of the emerging pathogens Arcobacter at an urban beach.
dc.type	Journal Article
utslib.citation.volume	171
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2020-12-15T03:05:46Z
pubs.publication-status	Published
pubs.volume	171

Abstract:

While the significance of Arcobacter in clinical settings grows, the ecological dynamics of potentially pathogenic Arcobacter in coastal marine environments remains unclear. In this study, we monitored the temporal dynamics of Arcobacter at an urban beach subject to significant stormwater input and wet weather sewer overflows (WWSO). Weekly monitoring of bacterial communities over 24 months using 16S rRNA amplicon sequencing revealed large, intermittent peaks in the relative abundance of Arcobacter. Quantitative PCR was subsequently employed to track absolute abundance of Arcobacter 23S rRNA gene copies, revealing peaks in abundance reaching up to 108 gene copies L-1, with these increases statistically correlated with stormwater and WWSO intrusion. Notably, peaks in Arcobacter abundance were poorly correlated with enterococci plate counts, and remained elevated for one week following heavy rainfall. Using oligotyping we discriminated single nucleotide variants (SNVs) within the Arcobacter population, revealing 10 distinct clusters of SNVs that we defined as Arcobacter "ecotypes", with each displaying distinct temporal dynamics. The most abundant ecotype during stormwater and modelled WWSO events displayed 16S rRNA sequence similarity to A. cryaerophilius, a species previously implicated in human illness. Our findings highlight the diverse environmental drivers of Arcobacter abundance within coastal settings and point to a potentially important, yet overlooked exposure risk of these potential pathogens to humans.

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http://hdl.handle.net/10453/144705