Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review.

Gasanov, U; Hughes, D; Hansbro, PM

Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review.

Gasanov, U Hughes, D Hansbro, PM

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Publisher:: OXFORD UNIV PRESS
Publication Type:: Journal Article
Citation:: FEMS microbiology reviews, 2005, 29, (5), pp. 851-875
Issue Date:: 2005-11

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Field	Value	Language
dc.contributor.author	Gasanov, U
dc.contributor.author	Hughes, D
dc.contributor.author	Hansbro, PM
dc.date.accessioned	2020-12-22T22:35:49Z
dc.date.available	2004-12-10
dc.date.available	2020-12-22T22:35:49Z
dc.date.issued	2005-11
dc.identifier.citation	FEMS microbiology reviews, 2005, 29, (5), pp. 851-875
dc.identifier.issn	0168-6445
dc.identifier.issn	1574-6976
dc.identifier.uri	http://hdl.handle.net/10453/144908
dc.description.abstract	Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	OXFORD UNIV PRESS
dc.relation.ispartof	FEMS microbiology reviews
dc.relation.isbasedon	10.1016/j.femsre.2004.12.002
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0605 Microbiology, 1108 Medical Microbiology
dc.subject.classification	Microbiology
dc.subject.mesh	Animals
dc.subject.mesh	Humans
dc.subject.mesh	Listeria
dc.subject.mesh	Listeria monocytogenes
dc.subject.mesh	Bacteriological Techniques
dc.subject.mesh	Bacterial Typing Techniques
dc.subject.mesh	Environmental Microbiology
dc.subject.mesh	Food Microbiology
dc.subject.mesh	Listeriosis
dc.subject.mesh	Animals
dc.subject.mesh	Bacterial Typing Techniques
dc.subject.mesh	Bacteriological Techniques
dc.subject.mesh	Environmental Microbiology
dc.subject.mesh	Food Microbiology
dc.subject.mesh	Humans
dc.subject.mesh	Listeria
dc.subject.mesh	Listeria monocytogenes
dc.subject.mesh	Listeriosis
dc.title	Methods for the isolation and identification of Listeria spp. and Listeria monocytogenes: a review.
dc.type	Journal Article
utslib.citation.volume	29
utslib.location.activity	England
utslib.for	0605 Microbiology
utslib.for	1108 Medical Microbiology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-12-22T22:35:40Z
pubs.issue	5
pubs.publication-status	Published
pubs.volume	29
utslib.citation.issue	5

Abstract:

Listeria monocytogenes is an important food-borne pathogen and is widely tested for in food, environmental and clinical samples. Identification traditionally involved culture methods based on selective enrichment and plating followed by the characterization of Listeria spp. based on colony morphology, sugar fermentation and haemolytic properties. These methods are the gold standard; but they are lengthy and may not be suitable for testing of foods with short shelf lives. As a result more rapid tests were developed based on antibodies (ELISA) or molecular techniques (PCR or DNA hybridization). While these tests possess equal sensitivity, they are rapid and allow testing to be completed within 48 h. More recently, molecular methods were developed that target RNA rather than DNA, such as RT-PCR, real time PCR or nucleic acid based sequence amplification (NASBA). These tests not only provide a measure of cell viability but they can also be used for quantitative analysis. In addition, a variety of tests are available for sub-species characterization, which are particularly useful in epidemiological investigations. Early typing methods differentiated isolates based on phenotypic markers, such as multilocus enzyme electrophoresis, phage typing and serotyping. These phenotypic typing methods are being replaced by molecular tests, which reflect genetic relationships between isolates and are more accurate. These new methods are currently mainly used in research but their considerable potential for routine testing in the future cannot be overlooked.

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http://hdl.handle.net/10453/144908