Transcutaneous immunization with combined cholera toxin and CpG adjuvant protects against Chlamydia muridarum genital tract infection.

Berry, LJ; Hickey, DK; Skelding, KA; Bao, S; Rendina, AM; Hansbro, PM; Gockel, CM; Beagley, KW

Transcutaneous immunization with combined cholera toxin and CpG adjuvant protects against Chlamydia muridarum genital tract infection.

Berry, LJ Hickey, DK Skelding, KA Bao, S Rendina, AM Hansbro, PM Gockel, CM Beagley, KW

Permalink

Publisher:: AMER SOC MICROBIOLOGY
Publication Type:: Journal Article
Citation:: Infection and immunity, 2004, 72, (2), pp. 1019-1028
Issue Date:: 2004-02

Closed Access

	Filename	Description	Size
	Transcutaneous immunisation with cholera toxin and CpG adjuvant protects against Cmu genital tract infection.pdf	Published version	1.58 MB		View/Open

Copyright Clearance Process

Recently Added
In Progress
Closed Access

This item is closed access and not available.

Full metadata record

Field	Value	Language
dc.contributor.author	Berry, LJ
dc.contributor.author	Hickey, DK
dc.contributor.author	Skelding, KA
dc.contributor.author	Bao, S
dc.contributor.author	Rendina, AM
dc.contributor.author	Hansbro, PM
dc.contributor.author	Gockel, CM
dc.contributor.author	Beagley, KW
dc.date.accessioned	2020-12-22T22:43:46Z
dc.date.available	2020-12-22T22:43:46Z
dc.date.issued	2004-02
dc.identifier.citation	Infection and immunity, 2004, 72, (2), pp. 1019-1028
dc.identifier.issn	0019-9567
dc.identifier.issn	1098-5522
dc.identifier.uri	http://hdl.handle.net/10453/144910
dc.description.abstract	Chlamydia trachomatis is a pathogen of the genital tract and ocular epithelium. Infection is established by the binding of the metabolically inert elementary body (EB) to epithelial cells. These are taken up by endocytosis into a membrane-bound vesicle termed an inclusion. The inclusion avoids fusion with host lysosomes, and the EBs differentiate into the metabolically active reticulate body (RB), which replicates by binary fission within the protected environment of the inclusion. During the extracellular EB stage of the C. trachomatis life cycle, antibody present in genital tract or ocular secretions can inhibit infection both in vivo and in tissue culture. The RB, residing within the intracellular inclusion, is not accessible to antibody, and resolution of infection at this stage requires a cell-mediated immune response mediated by gamma interferon-secreting Th1 cells. Thus, an ideal vaccine to protect against C. trachomatis genital tract infection should induce both antibody (immunoglobulin A [IgA] and IgG) responses in mucosal secretions to prevent infection by chlamydial EB and a strong Th1 response to limit ascending infection to the uterus and fallopian tubes. In the present study we show that transcutaneous immunization with major outer membrane protein (MOMP) in combination with both cholera toxin and CpG oligodeoxynucleotides elicits MOMP-specific IgG and IgA in vaginal and uterine lavage fluid, MOMP-specific IgG in serum, and gamma interferon-secreting T cells in reproductive tract-draining caudal and lumbar lymph nodes. This immunization protocol resulted in enhanced clearance of C. muridarum (C. trachomatis, mouse pneumonitis strain) following intravaginal challenge of BALB/c mice.
dc.format	Print
dc.language	eng
dc.publisher	AMER SOC MICROBIOLOGY
dc.relation.ispartof	Infection and immunity
dc.relation.isbasedon	10.1128/iai.72.2.1019-1028.2004
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	06 Biological Sciences, 07 Agricultural and Veterinary Sciences, 11 Medical and Health Sciences
dc.subject.classification	Microbiology
dc.subject.mesh	Vagina
dc.subject.mesh	Animals
dc.subject.mesh	Mice, Inbred BALB C
dc.subject.mesh	Mice
dc.subject.mesh	Chlamydia trachomatis
dc.subject.mesh	Chlamydia Infections
dc.subject.mesh	Genital Diseases, Female
dc.subject.mesh	Cholera Toxin
dc.subject.mesh	Porins
dc.subject.mesh	Immunoglobulin A
dc.subject.mesh	Immunoglobulin G
dc.subject.mesh	RNA, Messenger
dc.subject.mesh	Oligodeoxyribonucleotides
dc.subject.mesh	Bacterial Vaccines
dc.subject.mesh	Adjuvants, Immunologic
dc.subject.mesh	Immunization
dc.subject.mesh	Administration, Cutaneous
dc.subject.mesh	Female
dc.subject.mesh	Interferon-gamma
dc.subject.mesh	Adjuvants, Immunologic
dc.subject.mesh	Administration, Cutaneous
dc.subject.mesh	Animals
dc.subject.mesh	Bacterial Vaccines
dc.subject.mesh	Chlamydia Infections
dc.subject.mesh	Chlamydia trachomatis
dc.subject.mesh	Cholera Toxin
dc.subject.mesh	Female
dc.subject.mesh	Genital Diseases, Female
dc.subject.mesh	Immunization
dc.subject.mesh	Immunoglobulin A
dc.subject.mesh	Immunoglobulin G
dc.subject.mesh	Interferon-gamma
dc.subject.mesh	Mice
dc.subject.mesh	Mice, Inbred BALB C
dc.subject.mesh	Oligodeoxyribonucleotides
dc.subject.mesh	Porins
dc.subject.mesh	RNA, Messenger
dc.subject.mesh	Vagina
dc.title	Transcutaneous immunization with combined cholera toxin and CpG adjuvant protects against Chlamydia muridarum genital tract infection.
dc.type	Journal Article
utslib.citation.volume	72
utslib.location.activity	United States
utslib.for	06 Biological Sciences
utslib.for	07 Agricultural and Veterinary Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2020-12-22T22:43:36Z
pubs.issue	2
pubs.publication-status	Published
pubs.volume	72
utslib.citation.issue	2

Abstract:

Chlamydia trachomatis is a pathogen of the genital tract and ocular epithelium. Infection is established by the binding of the metabolically inert elementary body (EB) to epithelial cells. These are taken up by endocytosis into a membrane-bound vesicle termed an inclusion. The inclusion avoids fusion with host lysosomes, and the EBs differentiate into the metabolically active reticulate body (RB), which replicates by binary fission within the protected environment of the inclusion. During the extracellular EB stage of the C. trachomatis life cycle, antibody present in genital tract or ocular secretions can inhibit infection both in vivo and in tissue culture. The RB, residing within the intracellular inclusion, is not accessible to antibody, and resolution of infection at this stage requires a cell-mediated immune response mediated by gamma interferon-secreting Th1 cells. Thus, an ideal vaccine to protect against C. trachomatis genital tract infection should induce both antibody (immunoglobulin A [IgA] and IgG) responses in mucosal secretions to prevent infection by chlamydial EB and a strong Th1 response to limit ascending infection to the uterus and fallopian tubes. In the present study we show that transcutaneous immunization with major outer membrane protein (MOMP) in combination with both cholera toxin and CpG oligodeoxynucleotides elicits MOMP-specific IgG and IgA in vaginal and uterine lavage fluid, MOMP-specific IgG in serum, and gamma interferon-secreting T cells in reproductive tract-draining caudal and lumbar lymph nodes. This immunization protocol resulted in enhanced clearance of C. muridarum (C. trachomatis, mouse pneumonitis strain) following intravaginal challenge of BALB/c mice.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/144910