COPD is characterized by increased detection of Haemophilus influenzae, Streptococcus pneumoniae and a deficiency of Bacillus species.

Simpson, JL; Baines, KJ; Horvat, JC; Essilfie, A-T; Brown, AC; Tooze, M; McDonald, VM; Gibson, PG; Hansbro, PM

COPD is characterized by increased detection of Haemophilus influenzae, Streptococcus pneumoniae and a deficiency of Bacillus species.

Simpson, JL Baines, KJ Horvat, JC Essilfie, A-T Brown, AC Tooze, M McDonald, VM Gibson, PG Hansbro, PM

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Respirology (Carlton, Vic.), 2016, 21, (4), pp. 697-704
Issue Date:: 2016-05

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Field	Value	Language
dc.contributor.author	Simpson, JL
dc.contributor.author	Baines, KJ
dc.contributor.author	Horvat, JC
dc.contributor.author	Essilfie, A-T
dc.contributor.author	Brown, AC
dc.contributor.author	Tooze, M
dc.contributor.author	McDonald, VM
dc.contributor.author	Gibson, PG
dc.contributor.author	Hansbro, PM
dc.date.accessioned	2021-01-04T23:11:15Z
dc.date.available	2015-11-22
dc.date.available	2021-01-04T23:11:15Z
dc.date.issued	2016-05
dc.identifier.citation	Respirology (Carlton, Vic.), 2016, 21, (4), pp. 697-704
dc.identifier.issn	1323-7799
dc.identifier.issn	1440-1843
dc.identifier.uri	http://hdl.handle.net/10453/145075
dc.description.abstract	Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation and inflammation. Airway bacterial colonization is increased in COPD; however, the role of potentially pathogenic and non-pathogenic bacteria in the pathogenesis of disease is unclear. This study characterized the presence of bacteria in a well-characterized cohort of adults with COPD and healthy controls.Adults with COPD (n = 70) and healthy controls (n = 51) underwent clinical assessment and sputum induction. Sputum was dispersed, and total and differential cell counts were performed. Bacteria were cultured, identified and enumerated. Supernatants were assessed for neutrophil elastase (NE) and IL-1β. Common respiratory pathogens were also determined using real-time PCR.Participants with COPD had a typical neutrophilic inflammatory profile. The total load of bacteria was increased in COPD and was associated with poorer respiratory health status, as measured by the St George's Respiratory Questionnaire (Spearman's r = 0.336, P = 0.013). Significantly lower levels of culturable Bacillus species were identified compared with healthy controls. PCR analyses revealed increased rates of detection of potentially pathogenic bacteria with Haemophilus influenzae detection associated with higher sputum levels of NE and IL-1β, while Streptococcus pneumoniae was more common in male ex-smokers with emphysema and a deficit in diffusion capacity.Non-pathogenic and pathogenic bacteria were altered in the sputum of patients with COPD. These observations highlight the potential to identify treatment and management strategies that both target specific bacterial pathogens and restore the microbial balance, which may lead to reductions in inflammation and subsequent improvements in lung health.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation.ispartof	Respirology (Carlton, Vic.)
dc.relation.isbasedon	10.1111/resp.12734
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	11 Medical and Health Sciences
dc.subject.classification	Respiratory System
dc.subject.mesh	Neutrophils
dc.subject.mesh	Sputum
dc.subject.mesh	Humans
dc.subject.mesh	Bacillus
dc.subject.mesh	Haemophilus influenzae
dc.subject.mesh	Streptococcus pneumoniae
dc.subject.mesh	Pulmonary Disease, Chronic Obstructive
dc.subject.mesh	Leukocyte Elastase
dc.subject.mesh	Leukocyte Count
dc.subject.mesh	Case-Control Studies
dc.subject.mesh	Adult
dc.subject.mesh	Aged
dc.subject.mesh	Middle Aged
dc.subject.mesh	Female
dc.subject.mesh	Male
dc.subject.mesh	Interleukin-1beta
dc.subject.mesh	Young Adult
dc.subject.mesh	Bacterial Load
dc.subject.mesh	Surveys and Questionnaires
dc.subject.mesh	Adult
dc.subject.mesh	Aged
dc.subject.mesh	Bacillus
dc.subject.mesh	Bacterial Load
dc.subject.mesh	Case-Control Studies
dc.subject.mesh	Female
dc.subject.mesh	Haemophilus influenzae
dc.subject.mesh	Humans
dc.subject.mesh	Interleukin-1beta
dc.subject.mesh	Leukocyte Count
dc.subject.mesh	Leukocyte Elastase
dc.subject.mesh	Male
dc.subject.mesh	Middle Aged
dc.subject.mesh	Neutrophils
dc.subject.mesh	Pulmonary Disease, Chronic Obstructive
dc.subject.mesh	Sputum
dc.subject.mesh	Streptococcus pneumoniae
dc.subject.mesh	Surveys and Questionnaires
dc.subject.mesh	Young Adult
dc.title	COPD is characterized by increased detection of Haemophilus influenzae, Streptococcus pneumoniae and a deficiency of Bacillus species.
dc.type	Journal Article
utslib.citation.volume	21
utslib.location.activity	Australia
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-01-04T23:11:08Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	21
utslib.citation.issue	4

Abstract:

Chronic obstructive pulmonary disease (COPD) is characterized by progressive airflow limitation and inflammation. Airway bacterial colonization is increased in COPD; however, the role of potentially pathogenic and non-pathogenic bacteria in the pathogenesis of disease is unclear. This study characterized the presence of bacteria in a well-characterized cohort of adults with COPD and healthy controls.Adults with COPD (n = 70) and healthy controls (n = 51) underwent clinical assessment and sputum induction. Sputum was dispersed, and total and differential cell counts were performed. Bacteria were cultured, identified and enumerated. Supernatants were assessed for neutrophil elastase (NE) and IL-1β. Common respiratory pathogens were also determined using real-time PCR.Participants with COPD had a typical neutrophilic inflammatory profile. The total load of bacteria was increased in COPD and was associated with poorer respiratory health status, as measured by the St George's Respiratory Questionnaire (Spearman's r = 0.336, P = 0.013). Significantly lower levels of culturable Bacillus species were identified compared with healthy controls. PCR analyses revealed increased rates of detection of potentially pathogenic bacteria with Haemophilus influenzae detection associated with higher sputum levels of NE and IL-1β, while Streptococcus pneumoniae was more common in male ex-smokers with emphysema and a deficit in diffusion capacity.Non-pathogenic and pathogenic bacteria were altered in the sputum of patients with COPD. These observations highlight the potential to identify treatment and management strategies that both target specific bacterial pathogens and restore the microbial balance, which may lead to reductions in inflammation and subsequent improvements in lung health.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/145075