Organized Disassembly of Photosynthesis During Programmed Cell Death Mediated By Long Chain Bases.

Zavafer, A; González-Solís, A; Palacios-Bahena, S; Saucedo-García, M; Tapia de Aquino, C; Vázquez-Santana, S; King-Díaz, B; Gavilanes-Ruiz, M

Organized Disassembly of Photosynthesis During Programmed Cell Death Mediated By Long Chain Bases.

Zavafer, A González-Solís, A Palacios-Bahena, S Saucedo-García, M Tapia de Aquino, C Vázquez-Santana, S King-Díaz, B Gavilanes-Ruiz, M

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Publisher:: NATURE PUBLISHING GROUP
Publication Type:: Journal Article
Citation:: Scientific reports, 2020, 10, (1)
Issue Date:: 2020-06-25

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Field	Value	Language
dc.contributor.author	Zavafer, A
dc.contributor.author	González-Solís, A
dc.contributor.author	Palacios-Bahena, S
dc.contributor.author	Saucedo-García, M
dc.contributor.author	Tapia de Aquino, C
dc.contributor.author	Vázquez-Santana, S
dc.contributor.author	King-Díaz, B
dc.contributor.author	Gavilanes-Ruiz, M
dc.date.accessioned	2021-01-11T05:26:17Z
dc.date.available	2020-04-27
dc.date.available	2021-01-11T05:26:17Z
dc.date.issued	2020-06-25
dc.identifier.citation	Scientific reports, 2020, 10, (1)
dc.identifier.issn	2045-2322
dc.identifier.issn	2045-2322
dc.identifier.uri	http://hdl.handle.net/10453/145296
dc.description.abstract	In plants, pathogen triggered programmed cell death (PCD) is frequently mediated by polar lipid molecules referred as long chain bases (LCBs) or ceramides. PCD interceded by LCBs is a well-organized process where several cell organelles play important roles. In fact, light-dependent reactions in the chloroplast have been proposed as major players during PCD, however, the functional aspects of the chloroplast during PCD are largely unknown. For this reason, we investigated events that lead to disassembly of the chloroplast during PCD mediated by LCBs. To do so, LCB elevation was induced with Pseudomonas syringae pv. tomato (a non-host pathogen) or Fumonisin B1 in Phaseolus vulgaris. Then, we performed biochemical tests to detect PCD triggering events (phytosphingosine rises, MPK activation and H<sub>2</sub>O<sub>2</sub> generation) followed by chloroplast structural and functional tests. Observations of the chloroplast, via optical phenotyping methods combined with microscopy, indicated that the loss of photosynthetic linear electron transport coincides with the organized ultrastructure disassembly. In addition, structural changes occurred in parallel with accumulation of H<sub>2</sub>O<sub>2</sub> inside the chloroplast. These features revealed the collapse of chloroplast integrity and function as a mechanism leading to the irreversible execution of the PCD promoted by LCBs.
dc.format	Electronic
dc.language	eng
dc.publisher	NATURE PUBLISHING GROUP
dc.relation.ispartof	Scientific reports
dc.relation.isbasedon	10.1038/s41598-020-65186-8
dc.rights	info:eu-repo/semantics/openAccess
dc.subject.mesh	Chloroplasts
dc.subject.mesh	Pseudomonas syringae
dc.subject.mesh	Phaseolus
dc.subject.mesh	Lycopersicon esculentum
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Fumonisins
dc.subject.mesh	Lipids
dc.subject.mesh	Apoptosis
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Chloroplasts
dc.subject.mesh	Pseudomonas syringae
dc.subject.mesh	Phaseolus
dc.subject.mesh	Lycopersicon esculentum
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Fumonisins
dc.subject.mesh	Lipids
dc.subject.mesh	Apoptosis
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Apoptosis
dc.subject.mesh	Chloroplasts
dc.subject.mesh	Fumonisins
dc.subject.mesh	Hydrogen Peroxide
dc.subject.mesh	Lipids
dc.subject.mesh	Lycopersicon esculentum
dc.subject.mesh	Phaseolus
dc.subject.mesh	Photosynthesis
dc.subject.mesh	Pseudomonas syringae
dc.title	Organized Disassembly of Photosynthesis During Programmed Cell Death Mediated By Long Chain Bases.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	England
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
pubs.consider-herdc	false
dc.date.updated	2021-01-11T05:25:38Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	1

Abstract:

In plants, pathogen triggered programmed cell death (PCD) is frequently mediated by polar lipid molecules referred as long chain bases (LCBs) or ceramides. PCD interceded by LCBs is a well-organized process where several cell organelles play important roles. In fact, light-dependent reactions in the chloroplast have been proposed as major players during PCD, however, the functional aspects of the chloroplast during PCD are largely unknown. For this reason, we investigated events that lead to disassembly of the chloroplast during PCD mediated by LCBs. To do so, LCB elevation was induced with Pseudomonas syringae pv. tomato (a non-host pathogen) or Fumonisin B1 in Phaseolus vulgaris. Then, we performed biochemical tests to detect PCD triggering events (phytosphingosine rises, MPK activation and H₂O₂ generation) followed by chloroplast structural and functional tests. Observations of the chloroplast, via optical phenotyping methods combined with microscopy, indicated that the loss of photosynthetic linear electron transport coincides with the organized ultrastructure disassembly. In addition, structural changes occurred in parallel with accumulation of H₂O₂ inside the chloroplast. These features revealed the collapse of chloroplast integrity and function as a mechanism leading to the irreversible execution of the PCD promoted by LCBs.

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http://hdl.handle.net/10453/145296