Plasmon-Enhanced Surface-Enhanced Raman Scattering Mapping Concentrated on a Single Bead for Ultrasensitive and Multiplexed Immunoassay.

Lu, X; Ren, W; Hu, C; Liu, C; Li, Z

Plasmon-Enhanced Surface-Enhanced Raman Scattering Mapping Concentrated on a Single Bead for Ultrasensitive and Multiplexed Immunoassay.

Lu, X Ren, W

Hu, C Liu, C Li, Z

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Publisher:: AMER CHEMICAL SOC
Publication Type:: Journal Article
Citation:: Analytical chemistry, 2020, 92, (18), pp. 12387-12393
Issue Date:: 2020-09

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Field	Value	Language
dc.contributor.author	Lu, X
dc.contributor.author	Ren, W https://orcid.org/0000-0002-6537-6039
dc.contributor.author	Hu, C
dc.contributor.author	Liu, C
dc.contributor.author	Li, Z
dc.date.accessioned	2021-01-12T05:10:17Z
dc.date.available	2021-01-12T05:10:17Z
dc.date.issued	2020-09
dc.identifier.citation	Analytical chemistry, 2020, 92, (18), pp. 12387-12393
dc.identifier.issn	0003-2700
dc.identifier.issn	1520-6882
dc.identifier.uri	http://hdl.handle.net/10453/145338
dc.description.abstract	A single microbead (MB)-concentrated surface-enhanced Raman scattering (SERS) mapping strategy is proposed for ultrasensitive and multiplexed immunoassay with high precision. In this design, the SERS tags are specifically immobilized on the surface of a plasmonic gold nanoparticle (GNP) layer-coated single MB via target protein-mediated immune coupling. By this means, even ultralow target dosage can bring highly concentrated SERS tags on the confined small zone around the single MB, and the target-induced SERS signals are largely enhanced by the plasmonic layer, endowing the proposed strategy with ultrahigh sensitivity to quantify subpicogram per milliliter levels of proteins. Moreover, the per-pixel averaged SERS intensity is adopted for target quantitation through mapping the SERS signals around the MB's surface, achieving greatly improved reproducibility compared with traditional single-point measurement. Benefiting from the intrinsic merits of SERS mapping, this elegant strategy also enables multiplexed immunoassay on a single MB.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	AMER CHEMICAL SOC
dc.relation.ispartof	Analytical chemistry
dc.relation.isbasedon	10.1021/acs.analchem.0c02125
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	Plasmon-Enhanced Surface-Enhanced Raman Scattering Mapping Concentrated on a Single Bead for Ultrasensitive and Multiplexed Immunoassay.
dc.type	Journal Article
utslib.citation.volume	92
utslib.location.activity	United States
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-01-12T05:10:09Z
pubs.issue	18
pubs.publication-status	Published
pubs.volume	92
utslib.citation.issue	18

Abstract:

A single microbead (MB)-concentrated surface-enhanced Raman scattering (SERS) mapping strategy is proposed for ultrasensitive and multiplexed immunoassay with high precision. In this design, the SERS tags are specifically immobilized on the surface of a plasmonic gold nanoparticle (GNP) layer-coated single MB via target protein-mediated immune coupling. By this means, even ultralow target dosage can bring highly concentrated SERS tags on the confined small zone around the single MB, and the target-induced SERS signals are largely enhanced by the plasmonic layer, endowing the proposed strategy with ultrahigh sensitivity to quantify subpicogram per milliliter levels of proteins. Moreover, the per-pixel averaged SERS intensity is adopted for target quantitation through mapping the SERS signals around the MB's surface, achieving greatly improved reproducibility compared with traditional single-point measurement. Benefiting from the intrinsic merits of SERS mapping, this elegant strategy also enables multiplexed immunoassay on a single MB.

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http://hdl.handle.net/10453/145338