Revealing changes in the microbiome of Symbiodiniaceae under thermal stress.

Camp, EF; Kahlke, T; Nitschke, MR; Varkey, D; Fisher, NL; Fujise, L; Goyen, S; Hughes, DJ; Lawson, CA; Ros, M; Woodcock, S; Xiao, K; Leggat, W; Suggett, DJ

Revealing changes in the microbiome of Symbiodiniaceae under thermal stress.

Camp, EF Kahlke, T

Nitschke, MR Varkey, D Fisher, NL Fujise, L Goyen, S

Hughes, DJ Lawson, CA Ros, M

Woodcock, S

Xiao, K Leggat, W Suggett, DJ

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Publisher:: WILEY
Publication Type:: Journal Article
Citation:: Environmental microbiology, 2020, 22, (4), pp. 1294-1309
Issue Date:: 2020-04

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Field	Value	Language
dc.contributor.author	Camp, EF
dc.contributor.author	Kahlke, T https://orcid.org/0000-0002-9762-6573
dc.contributor.author	Nitschke, MR
dc.contributor.author	Varkey, D
dc.contributor.author	Fisher, NL
dc.contributor.author	Fujise, L
dc.contributor.author	Goyen, S https://orcid.org/0000-0003-3691-4640
dc.contributor.author	Hughes, DJ
dc.contributor.author	Lawson, CA
dc.contributor.author	Ros, M https://orcid.org/0000-0002-1140-0101
dc.contributor.author	Woodcock, S https://orcid.org/0000-0003-4903-3164
dc.contributor.author	Xiao, K
dc.contributor.author	Leggat, W
dc.contributor.author	Suggett, DJ
dc.date.accessioned	2021-01-14T05:11:42Z
dc.date.available	2020-01-27
dc.date.available	2021-01-14T05:11:42Z
dc.date.issued	2020-04
dc.identifier.citation	Environmental microbiology, 2020, 22, (4), pp. 1294-1309
dc.identifier.issn	1462-2912
dc.identifier.issn	1462-2920
dc.identifier.uri	http://hdl.handle.net/10453/145433
dc.description.abstract	Symbiodiniaceae are a diverse family of marine dinoflagellates, well known as coral endosymbionts. Isolation and in vitro culture of Symbiodiniaceae strains for physiological studies is a widely adopted tool, especially in the context of understanding how environmental stress perturbs Symbiodiniaceae cell functioning. While the bacterial microbiomes of corals often correlate with coral health, the bacterial communities co-cultured with Symbiodiniaceae isolates have been largely overlooked, despite the potential of bacteria to significantly influence the emergent physiological properties of Symbiodiniaceae cultures. We examined the physiological response to heat stress by Symbiodiniaceae isolates (spanning three genera) with well-described thermal tolerances, and combined these observations with matched changes in bacterial composition and abundance through 16S rRNA metabarcoding. Under thermal stress, there were Symbiodiniaceae strain-specific changes in maximum quantum yield of photosystem II (proxy for health) and growth rates that were accompanied by changes in the relative abundance of multiple Symbiodiniaceae-specific bacteria. However, there were no Symbiodiniaceae-independent signatures of bacterial community reorganisation under heat stress. Notably, the thermally tolerant Durusdinium trenchii (ITS2 major profile D1a) had the most stable bacterial community under heat stress. Ultimately, this study highlights the complexity of Symbiodiniaceae-bacteria interactions and provides a first step towards uncoupling their relative contributions towards Symbiodiniaceae physiological functioning.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	WILEY
dc.relation	http://purl.org/au-research/grants/arc/DP160100271
dc.relation	http://purl.org/au-research/grants/arc/DE190100142
dc.relation.ispartof	Environmental microbiology
dc.relation.isbasedon	10.1111/1462-2920.14935
dc.rights	info:eu-repo/semantics/restrictedAccess
dc.subject	0603 Evolutionary Biology, 0605 Microbiology
dc.subject.classification	Microbiology
dc.subject.mesh	Animals
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Bacteria
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Heat-Shock Response
dc.subject.mesh	Microbiota
dc.subject.mesh	Animals
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Bacteria
dc.subject.mesh	RNA, Ribosomal, 16S
dc.subject.mesh	Heat-Shock Response
dc.subject.mesh	Microbiota
dc.subject.mesh	Animals
dc.subject.mesh	Bacteria
dc.subject.mesh	Dinoflagellida
dc.subject.mesh	Heat-Shock Response
dc.subject.mesh	Microbiota
dc.subject.mesh	RNA, Ribosomal, 16S
dc.title	Revealing changes in the microbiome of Symbiodiniaceae under thermal stress.
dc.type	Journal Article
utslib.citation.volume	22
utslib.location.activity	England
utslib.for	0603 Evolutionary Biology
utslib.for	0605 Microbiology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Mathematical and Physical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney/DVC (Resources)
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-01-14T05:11:31Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	22
utslib.citation.issue	4

Abstract:

Symbiodiniaceae are a diverse family of marine dinoflagellates, well known as coral endosymbionts. Isolation and in vitro culture of Symbiodiniaceae strains for physiological studies is a widely adopted tool, especially in the context of understanding how environmental stress perturbs Symbiodiniaceae cell functioning. While the bacterial microbiomes of corals often correlate with coral health, the bacterial communities co-cultured with Symbiodiniaceae isolates have been largely overlooked, despite the potential of bacteria to significantly influence the emergent physiological properties of Symbiodiniaceae cultures. We examined the physiological response to heat stress by Symbiodiniaceae isolates (spanning three genera) with well-described thermal tolerances, and combined these observations with matched changes in bacterial composition and abundance through 16S rRNA metabarcoding. Under thermal stress, there were Symbiodiniaceae strain-specific changes in maximum quantum yield of photosystem II (proxy for health) and growth rates that were accompanied by changes in the relative abundance of multiple Symbiodiniaceae-specific bacteria. However, there were no Symbiodiniaceae-independent signatures of bacterial community reorganisation under heat stress. Notably, the thermally tolerant Durusdinium trenchii (ITS2 major profile D1a) had the most stable bacterial community under heat stress. Ultimately, this study highlights the complexity of Symbiodiniaceae-bacteria interactions and provides a first step towards uncoupling their relative contributions towards Symbiodiniaceae physiological functioning.

Please use this identifier to cite or link to this item:

http://hdl.handle.net/10453/145433