Rapid GFAP and Iba1 Expression Changes in the Female Rat Brain following Spinal Cord Injury

Mandwie, M; Piper, J; Gorrie, C; Keay, K; Musumeci, G; Al-Badri, G; Castorina, A

Rapid GFAP and Iba1 Expression Changes in the Female Rat Brain following Spinal Cord Injury

Mandwie, M Piper, J Gorrie, C Keay, K Musumeci, G Al-Badri, G Castorina, A

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Publisher:: Medknow Publications
Publication Type:: Journal Article
Citation:: Neural Regeneration Research, 2021
Issue Date:: 2021-01-11

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Field	Value	Language
dc.contributor.author	Mandwie, M
dc.contributor.author	Piper, J
dc.contributor.author	Gorrie, C
dc.contributor.author	Keay, K
dc.contributor.author	Musumeci, G
dc.contributor.author	Al-Badri, G
dc.contributor.author	Castorina, A https://orcid.org/0000-0001-7037-759X
dc.date.accessioned	2021-01-21T22:50:39Z
dc.date.available	2021-01-11
dc.date.available	2021-01-21T22:50:39Z
dc.date.issued	2021-01-11
dc.identifier.citation	Neural Regeneration Research, 2021
dc.identifier.issn	1673-5374
dc.identifier.uri	http://hdl.handle.net/10453/145532
dc.description.abstract	Spinal cord injury (SCI) is a devastating condition often associated with sleep disorders, mood change and depression. Evidence suggests that rapid changes to supporting glia may predispose individuals with SCI to such comorbidities. Here, we interrogated the expression of astrocyte- and microglial-specific markers glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) in the rat brain in the first 24 hours following spinal cord injury (SCI). Female Sprague Dawley rats underwent thoracic laminectomy; half of the rats received a mild contusion injury at the level of the T10 vertebral body (SCI group), the other half did not (Sham group). Twenty-four hours post-surgery the rats were sacrificed, and the amygdala, periaqueductal grey, prefrontal cortex, hypothalamus, lateral thalamus, hippocampus (dorsal and ventral) were collected. GFAP and Iba1 mRNA and protein levels were measured by real-time qPCR and Western blot. In SCI rats, GFAP mRNA and protein expression increased in the amygdala and hypothalamus (p<0.05). In contrast, gene and protein expression decreased in the thalamus (p<0.01) and dorsal hippocampus (p<0.05 and *p<0.01, respectively). Interestingly, Iba1 transcripts and proteins were significantly diminished only in the dorsal (p<0.05 and *p<0.01, respectively) and ventral hippocampus, where gene expression diminished (p<0.05 for both mRNA and protein). Considered together, these findings demonstrate that as early as 24 hours post-SCI there are region-specific disruptions of GFAP and Iba1 transcript and protein levels in higher brain regions.
dc.language	en
dc.publisher	Medknow Publications
dc.relation.ispartof	Neural Regeneration Research
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	1109 Neurosciences
dc.title	Rapid GFAP and Iba1 Expression Changes in the Female Rat Brain following Spinal Cord Injury
dc.type	Journal Article
utslib.location.activity	Australia
utslib.for	1109 Neurosciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	open_access	*
pubs.consider-herdc	true
dc.date.updated	2021-01-21T22:49:53Z
pubs.publication-status	Accepted

Abstract:

Spinal cord injury (SCI) is a devastating condition often associated with sleep disorders, mood change and depression. Evidence suggests that rapid changes to supporting glia may predispose individuals with SCI to such comorbidities. Here, we interrogated the expression of astrocyte- and microglial-specific markers glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba1) in the rat brain in the first 24 hours following spinal cord injury (SCI). Female Sprague Dawley rats underwent thoracic laminectomy; half of the rats received a mild contusion injury at the level of the T10 vertebral body (SCI group), the other half did not (Sham group). Twenty-four hours post-surgery the rats were sacrificed, and the amygdala, periaqueductal grey, prefrontal cortex, hypothalamus, lateral thalamus, hippocampus (dorsal and ventral) were collected. GFAP and Iba1 mRNA and protein levels were measured by real-time qPCR and Western blot. In SCI rats, GFAP mRNA and protein expression increased in the amygdala and hypothalamus (*p<0.05). In contrast, gene and protein expression decreased in the thalamus (**p<0.01) and dorsal hippocampus (*p<0.05 and **p<0.01, respectively). Interestingly, Iba1 transcripts and proteins were significantly diminished only in the dorsal (*p<0.05 and **p<0.01, respectively) and ventral hippocampus, where gene expression diminished (*p<0.05 for both mRNA and protein). Considered together, these findings demonstrate that as early as 24 hours post-SCI there are region-specific disruptions of GFAP and Iba1 transcript and protein levels in higher brain regions.

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http://hdl.handle.net/10453/145532