Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.

Mohamed, A; Chan, H; Luhur, J; Bauda, E; Gallet, B; Morlot, C; Cole, L; Awad, M; Crawford, S; Lyras, D; Rudner, DZ; Rodrigues, CDA

Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.

Mohamed, A Chan, H

Luhur, J Bauda, E Gallet, B Morlot, C Cole, L

Awad, M Crawford, S Lyras, D Rudner, DZ Rodrigues, CDA

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Publisher:: Elsevier
Publication Type:: Journal Article
Citation:: Developmental Cell, 2021, 56, (1), pp. 36-51.e5
Issue Date:: 2021

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Field	Value	Language
dc.contributor.author	Mohamed, A
dc.contributor.author	Chan, H https://orcid.org/0000-0002-5194-0006
dc.contributor.author	Luhur, J
dc.contributor.author	Bauda, E
dc.contributor.author	Gallet, B
dc.contributor.author	Morlot, C
dc.contributor.author	Cole, L https://orcid.org/0000-0001-9206-9272
dc.contributor.author	Awad, M
dc.contributor.author	Crawford, S
dc.contributor.author	Lyras, D
dc.contributor.author	Rudner, DZ
dc.contributor.author	Rodrigues, CDA
dc.date.accessioned	2021-02-19T00:18:44Z
dc.date.available	2020-12-07
dc.date.available	2021-02-19T00:18:44Z
dc.date.issued	2021
dc.identifier.citation	Developmental Cell, 2021, 56, (1), pp. 36-51.e5
dc.identifier.issn	1534-5807
dc.identifier.issn	1878-1551
dc.identifier.uri	http://hdl.handle.net/10453/146221
dc.description.abstract	Asymmetric division, a hallmark of endospore development, generates two cells, a larger mother cell and a smaller forespore. Approximately 75% of the forespore chromosome must be translocated across the division septum into the forespore by the DNA translocase SpoIIIE. Asymmetric division also triggers cell-specific transcription, which initiates septal peptidoglycan remodeling involving synthetic and hydrolytic enzymes. How these processes are coordinated has remained a mystery. Using Bacillus subtilis, we identified factors that revealed the link between chromosome translocation and peptidoglycan remodeling. In cells lacking these factors, the asymmetric septum retracts, resulting in forespore cytoplasmic leakage and loss of DNA translocation. Importantly, these phenotypes depend on septal peptidoglycan hydrolysis. Our data support a model in which SpoIIIE is anchored at the edge of a septal pore, stabilized by newly synthesized peptidoglycan and protein-protein interactions across the septum. Together, these factors ensure coordination between chromosome translocation and septal peptidoglycan remodeling to maintain spore development.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Elsevier
dc.relation	http://purl.org/au-research/grants/arc/DP190100793
dc.relation	University of Technology Sydney
dc.relation.ispartof	Developmental Cell
dc.relation.isbasedon	10.1016/j.devcel.2020.12.006
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	06 Biological Sciences, 11 Medical and Health Sciences
dc.subject.classification	Developmental Biology
dc.title	Chromosome Segregation and Peptidoglycan Remodeling Are Coordinated at a Highly Stabilized Septal Pore to Maintain Bacterial Spore Development.
dc.type	Journal Article
utslib.citation.volume	56
utslib.location.activity	United States
utslib.for	06 Biological Sciences
utslib.for	11 Medical and Health Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
pubs.consider-herdc	false
dc.date.updated	2021-02-19T00:17:53Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	56
utslib.citation.issue	1

Abstract:

Asymmetric division, a hallmark of endospore development, generates two cells, a larger mother cell and a smaller forespore. Approximately 75% of the forespore chromosome must be translocated across the division septum into the forespore by the DNA translocase SpoIIIE. Asymmetric division also triggers cell-specific transcription, which initiates septal peptidoglycan remodeling involving synthetic and hydrolytic enzymes. How these processes are coordinated has remained a mystery. Using Bacillus subtilis, we identified factors that revealed the link between chromosome translocation and peptidoglycan remodeling. In cells lacking these factors, the asymmetric septum retracts, resulting in forespore cytoplasmic leakage and loss of DNA translocation. Importantly, these phenotypes depend on septal peptidoglycan hydrolysis. Our data support a model in which SpoIIIE is anchored at the edge of a septal pore, stabilized by newly synthesized peptidoglycan and protein-protein interactions across the septum. Together, these factors ensure coordination between chromosome translocation and septal peptidoglycan remodeling to maintain spore development.

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http://hdl.handle.net/10453/146221