In-situ Neutron Reflectometry Study on Adsorption of Glucose Oxidase at Mesoporous Aluminum Oxide Film.

Yamaguchi, A; Katayama, K; Holt, SA

In-situ Neutron Reflectometry Study on Adsorption of Glucose Oxidase at Mesoporous Aluminum Oxide Film.

Yamaguchi, A Katayama, K Holt, SA

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Publisher:: JAPAN SOC ANALYTICAL CHEMISTRY
Publication Type:: Journal Article
Citation:: Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2020, 36, (11), pp. 1331-1336
Issue Date:: 2020-11

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Field	Value	Language
dc.contributor.author	Yamaguchi, A
dc.contributor.author	Katayama, K
dc.contributor.author	Holt, SA
dc.date.accessioned	2021-02-19T22:13:59Z
dc.date.available	2021-02-19T22:13:59Z
dc.date.issued	2020-11
dc.identifier.citation	Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2020, 36, (11), pp. 1331-1336
dc.identifier.issn	0910-6340
dc.identifier.issn	1348-2246
dc.identifier.uri	http://hdl.handle.net/10453/146231
dc.description.abstract	In the present study, the adsorption of glucose oxidase (GOD) to a mesoporous aluminum oxide (MAO) film was examined with in-situ neutron reflectometry (NR) measurements. The MAO film was deposited on a cover glass slip and a Si disc, and its pore structure was characterized by X-ray reflectometry (XRR) and NR. The Si disc with MAO film was applied for an in-situ NR experiment, and its NR profiles before/after adsorption of GOD were continuously measured with a flow cell. The results indicated that the negatively-charged GOD molecules hardly penetrate into the narrow pore channel (pore diameter = ca. 10 nm) with opposite surface charge.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	JAPAN SOC ANALYTICAL CHEMISTRY
dc.relation.ispartof	Analytical sciences : the international journal of the Japan Society for Analytical Chemistry
dc.relation.isbasedon	10.2116/analsci.20p160
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	0301 Analytical Chemistry, 0399 Other Chemical Sciences
dc.subject.classification	Analytical Chemistry
dc.title	In-situ Neutron Reflectometry Study on Adsorption of Glucose Oxidase at Mesoporous Aluminum Oxide Film.
dc.type	Journal Article
utslib.citation.volume	36
utslib.location.activity	Japan
utslib.for	0301 Analytical Chemistry
utslib.for	0399 Other Chemical Sciences
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-02-19T22:13:40Z
pubs.issue	11
pubs.publication-status	Published
pubs.volume	36
utslib.citation.issue	11

Abstract:

In the present study, the adsorption of glucose oxidase (GOD) to a mesoporous aluminum oxide (MAO) film was examined with in-situ neutron reflectometry (NR) measurements. The MAO film was deposited on a cover glass slip and a Si disc, and its pore structure was characterized by X-ray reflectometry (XRR) and NR. The Si disc with MAO film was applied for an in-situ NR experiment, and its NR profiles before/after adsorption of GOD were continuously measured with a flow cell. The results indicated that the negatively-charged GOD molecules hardly penetrate into the narrow pore channel (pore diameter = ca. 10 nm) with opposite surface charge.

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http://hdl.handle.net/10453/146231