Comparative assessment of genomic DNA extraction processes for Plasmodium: Identifying the appropriate method.

Mann, R; Sharma, S; Mishra, N; Valecha, N; Anvikar, AR

Comparative assessment of genomic DNA extraction processes for Plasmodium: Identifying the appropriate method.

Mann, R

Sharma, S Mishra, N Valecha, N Anvikar, AR

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Publisher:: MEDKNOW PUBLICATIONS & MEDIA PVT LTD
Publication Type:: Journal Article
Citation:: Journal of vector borne diseases, 2015, 52, (4), pp. 273-280
Issue Date:: 2015-12

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Field	Value	Language
dc.contributor.author	Mann, R https://orcid.org/0000-0003-4772-0139
dc.contributor.author	Sharma, S
dc.contributor.author	Mishra, N
dc.contributor.author	Valecha, N
dc.contributor.author	Anvikar, AR
dc.date.accessioned	2021-03-01T03:25:21Z
dc.date.available	2021-03-01T03:25:21Z
dc.date.issued	2015-12
dc.identifier.citation	Journal of vector borne diseases, 2015, 52, (4), pp. 273-280
dc.identifier.issn	0972-9062
dc.identifier.uri	http://hdl.handle.net/10453/146558
dc.description.abstract	Plasmodium DNA, in addition to being used for molecular diagnosis of malaria, find utility in monitoring patient responses to antimalarial drugs, drug resistance studies, genotyping and sequencing purposes. Over the years, numerous protocols have been proposed for extracting Plasmodium DNA from a variety of sources. Given that DNA isolation is fundamental to successful molecular studies, here we review the most commonly used methods for Plasmodium genomic DNA isolation, emphasizing their pros and cons. A comparison of these existing methods has been made, to evaluate their appropriateness for use in different applications and identify the method suitable for a particular laboratory based study. Selection of a suitable and accessible DNA extraction method for Plasmodium requires consideration of many factors, the most important being sensitivity, cost-effectiveness and, purity and stability of isolated DNA. Need of the hour is to accentuate on the development of a method that upholds well on all these parameters.
dc.format	Print
dc.language	eng
dc.publisher	MEDKNOW PUBLICATIONS & MEDIA PVT LTD
dc.relation.ispartof	Journal of vector borne diseases
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject.classification	Tropical Medicine
dc.subject.mesh	Humans
dc.subject.mesh	Plasmodium
dc.subject.mesh	Malaria
dc.subject.mesh	DNA, Protozoan
dc.subject.mesh	Genotype
dc.subject.mesh	Analytic Sample Preparation Methods
dc.subject.mesh	Analytic Sample Preparation Methods
dc.subject.mesh	DNA, Protozoan
dc.subject.mesh	Genotype
dc.subject.mesh	Humans
dc.subject.mesh	Malaria
dc.subject.mesh	Plasmodium
dc.title	Comparative assessment of genomic DNA extraction processes for Plasmodium: Identifying the appropriate method.
dc.type	Journal Article
utslib.citation.volume	52
utslib.location.activity	India
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-03-01T03:25:20Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	52
utslib.citation.issue	4

Abstract:

Plasmodium DNA, in addition to being used for molecular diagnosis of malaria, find utility in monitoring patient responses to antimalarial drugs, drug resistance studies, genotyping and sequencing purposes. Over the years, numerous protocols have been proposed for extracting Plasmodium DNA from a variety of sources. Given that DNA isolation is fundamental to successful molecular studies, here we review the most commonly used methods for Plasmodium genomic DNA isolation, emphasizing their pros and cons. A comparison of these existing methods has been made, to evaluate their appropriateness for use in different applications and identify the method suitable for a particular laboratory based study. Selection of a suitable and accessible DNA extraction method for Plasmodium requires consideration of many factors, the most important being sensitivity, cost-effectiveness and, purity and stability of isolated DNA. Need of the hour is to accentuate on the development of a method that upholds well on all these parameters.

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http://hdl.handle.net/10453/146558