Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs.

Maire, J; Girvan, SK; Barkla, SE; Perez-Gonzalez, A; Suggett, DJ; Blackall, LL; van Oppen, MJH

Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs.

Maire, J Girvan, SK Barkla, SE Perez-Gonzalez, A Suggett, DJ Blackall, LL van Oppen, MJH

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Publisher:: Springer Science and Business Media LLC
Publication Type:: Journal Article
Citation:: The ISME journal, 2021, pp. 1-15
Issue Date:: 2021-02-08

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Field	Value	Language
dc.contributor.author	Maire, J
dc.contributor.author	Girvan, SK
dc.contributor.author	Barkla, SE
dc.contributor.author	Perez-Gonzalez, A
dc.contributor.author	Suggett, DJ
dc.contributor.author	Blackall, LL
dc.contributor.author	van Oppen, MJH
dc.date.accessioned	2021-03-01T21:45:22Z
dc.date.available	2021-01-19
dc.date.available	2021-03-01T21:45:22Z
dc.date.issued	2021-02-08
dc.identifier.citation	The ISME journal, 2021, pp. 1-15
dc.identifier.issn	1751-7362
dc.identifier.issn	1751-7370
dc.identifier.uri	http://hdl.handle.net/10453/146596
dc.description.abstract	Corals house a variety of microorganisms which they depend on for their survival, including endosymbiotic dinoflagellates (Symbiodiniaceae) and bacteria. While cnidarian-microorganism interactions are widely studied, Symbiodiniaceae-bacteria interactions are only just beginning to receive attention. Here, we describe the localization and composition of the bacterial communities associated with cultures of 11 Symbiodiniaceae strains from nine species and six genera. Three-dimensional confocal laser scanning and electron microscopy revealed bacteria are present inside the Symbiodiniaceae cells as well as closely associated with their external cell surface. Bacterial pure cultures and 16S rRNA gene metabarcoding from Symbiodiniaceae cultures highlighted distinct and highly diverse bacterial communities occur intracellularly, closely associated with the Symbiodiniaceae outer cell surface and loosely associated (i.e., in the surrounding culture media). The intracellular bacteria are highly conserved across Symbiodiniaceae species, suggesting they may be involved in Symbiodiniaceae physiology. Our findings provide unique new insights into the biology of Symbiodiniaceae.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Springer Science and Business Media LLC
dc.relation.ispartof	The ISME journal
dc.relation.isbasedon	10.1038/s41396-021-00902-4
dc.rights	info:eu-repo/semantics/closedAccess
dc.subject	05 Environmental Sciences, 06 Biological Sciences, 10 Technology
dc.subject.classification	Microbiology
dc.title	Intracellular bacteria are common and taxonomically diverse in cultured and in hospite algal endosymbionts of coral reefs.
dc.type	Journal Article
utslib.location.activity	England
utslib.for	05 Environmental Sciences
utslib.for	06 Biological Sciences
utslib.for	10 Technology
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
pubs.organisational-group	/University of Technology Sydney
utslib.copyright.status	closed_access	*
dc.date.updated	2021-03-01T21:45:18Z
pubs.publication-status	Published

Abstract:

Corals house a variety of microorganisms which they depend on for their survival, including endosymbiotic dinoflagellates (Symbiodiniaceae) and bacteria. While cnidarian-microorganism interactions are widely studied, Symbiodiniaceae-bacteria interactions are only just beginning to receive attention. Here, we describe the localization and composition of the bacterial communities associated with cultures of 11 Symbiodiniaceae strains from nine species and six genera. Three-dimensional confocal laser scanning and electron microscopy revealed bacteria are present inside the Symbiodiniaceae cells as well as closely associated with their external cell surface. Bacterial pure cultures and 16S rRNA gene metabarcoding from Symbiodiniaceae cultures highlighted distinct and highly diverse bacterial communities occur intracellularly, closely associated with the Symbiodiniaceae outer cell surface and loosely associated (i.e., in the surrounding culture media). The intracellular bacteria are highly conserved across Symbiodiniaceae species, suggesting they may be involved in Symbiodiniaceae physiology. Our findings provide unique new insights into the biology of Symbiodiniaceae.

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http://hdl.handle.net/10453/146596