Flow cytometric assessment of Cd genotoxicity in three plants with different metal accumulation and detoxification capacities

Monteiro, MS; Rodriguez, E; Loureiro, J; Mann, RM; Soares, AMVM; Santos, C

Flow cytometric assessment of Cd genotoxicity in three plants with different metal accumulation and detoxification capacities

Monteiro, MS Rodriguez, E Loureiro, J Mann, RM

Soares, AMVM Santos, C

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Publication Type:: Journal Article
Citation:: Ecotoxicology and Environmental Safety, 2010, 73 (6), pp. 1231 - 1237
Issue Date:: 2010-09-01

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Field	Value	Language
dc.contributor.author	Monteiro, MS	en_US
dc.contributor.author	Rodriguez, E	en_US
dc.contributor.author	Loureiro, J	en_US
dc.contributor.author	Mann, RM https://orcid.org/0000-0001-7696-0751	en_US
dc.contributor.author	Soares, AMVM	en_US
dc.contributor.author	Santos, C	en_US
dc.date.available	2010-06-26	en_US
dc.date.issued	2010-09-01	en_US
dc.identifier.citation	Ecotoxicology and Environmental Safety, 2010, 73 (6), pp. 1231 - 1237	en_US
dc.identifier.issn	0147-6513	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14776
dc.description.abstract	Cadmium (Cd) is a widespread environmental contaminant, strongly mutagenic and known to cause DNA damage in plants. In this work, flow cytometry (FCM) was applied to determine if in vivo exposure to Cd would induce genotoxic effects at the genome level. The hyper-accumulator Thlaspi caerulescens (J. & C. Presl), the related non-accumulator Thlaspi arvense L. and the accumulator crop species Lactuca sativa L. were germinated in distilled water and grown in modified Hoagland's medium with increasing concentrations of Cd(NO3)2 (0, 1, 10 and 100γM). After 28 days of exposure, shoot and root growth was recorded and the tissues were harvested for Cd and FCM analysis. In general, roots from treated plants contained higher content of Cd than leaves and growth inhibition was observed in the treated plants. Nuclear DNA content was estimated and the G0/G1 full peak coefficient of variation (FPCV), as an indicator of clastogenic damage, was recorded. In T. arvense and T. caerulescens no significant differences were detected between control and exposed plants. Leaves of L. sativa exposed to 10γM Cd presented a statistically significant increase in FPCV values in comparison with the control group. Furthermore, roots exposed to 100γM Cd presented a reduction in nuclear DNA content and an increase in FPCV when compared to the control. FCM data indicates that no major DNA damage was induced on both Cd-exposed Thlaspi species and L. sativa leaves. On the contrary, results obtained with L. sativa roots suggests clastogenic damage in these organs exposed to 100γM of Cd. © 2010 Elsevier Inc.	en_US
dc.relation.ispartof	Ecotoxicology and Environmental Safety	en_US
dc.relation.isbasedon	10.1016/j.ecoenv.2010.06.020	en_US
dc.subject.classification	Strategic, Defence & Security Studies	en_US
dc.subject.mesh	Plants	en_US
dc.subject.mesh	Lettuce	en_US
dc.subject.mesh	Thlaspi	en_US
dc.subject.mesh	Plant Leaves	en_US
dc.subject.mesh	Plant Roots	en_US
dc.subject.mesh	DNA Damage	en_US
dc.subject.mesh	Cadmium	en_US
dc.subject.mesh	Environmental Pollutants	en_US
dc.subject.mesh	Mutagens	en_US
dc.subject.mesh	Flow Cytometry	en_US
dc.subject.mesh	Species Specificity	en_US
dc.subject.mesh	Dose-Response Relationship, Drug	en_US
dc.subject.mesh	Plant Development	en_US
dc.title	Flow cytometric assessment of Cd genotoxicity in three plants with different metal accumulation and detoxification capacities	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	73	en_US
utslib.for	0607 Plant Biology	en_US
utslib.for	03 Chemical Sciences	en_US
utslib.for	05 Environmental Sciences	en_US
utslib.for	11 Medical and Health Sciences	en_US
dc.location.activity	ISI:000248977500006
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	73	en_US

Abstract:

Cadmium (Cd) is a widespread environmental contaminant, strongly mutagenic and known to cause DNA damage in plants. In this work, flow cytometry (FCM) was applied to determine if in vivo exposure to Cd would induce genotoxic effects at the genome level. The hyper-accumulator Thlaspi caerulescens (J. & C. Presl), the related non-accumulator Thlaspi arvense L. and the accumulator crop species Lactuca sativa L. were germinated in distilled water and grown in modified Hoagland's medium with increasing concentrations of Cd(NO3)2 (0, 1, 10 and 100γM). After 28 days of exposure, shoot and root growth was recorded and the tissues were harvested for Cd and FCM analysis. In general, roots from treated plants contained higher content of Cd than leaves and growth inhibition was observed in the treated plants. Nuclear DNA content was estimated and the G0/G1 full peak coefficient of variation (FPCV), as an indicator of clastogenic damage, was recorded. In T. arvense and T. caerulescens no significant differences were detected between control and exposed plants. Leaves of L. sativa exposed to 10γM Cd presented a statistically significant increase in FPCV values in comparison with the control group. Furthermore, roots exposed to 100γM Cd presented a reduction in nuclear DNA content and an increase in FPCV when compared to the control. FCM data indicates that no major DNA damage was induced on both Cd-exposed Thlaspi species and L. sativa leaves. On the contrary, results obtained with L. sativa roots suggests clastogenic damage in these organs exposed to 100γM of Cd. © 2010 Elsevier Inc.

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http://hdl.handle.net/10453/14776