Liensinine prevents vascular inflammation by attenuating inflammatory mediators and modulating vsmc function

Jun, MY; Karki, R; Paudel, KR; Panth, N; Devkota, HP; Kim, DW

Liensinine prevents vascular inflammation by attenuating inflammatory mediators and modulating vsmc function

Jun, MY Karki, R Paudel, KR Panth, N Devkota, HP Kim, DW

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Publisher:: MDPI
Publication Type:: Journal Article
Citation:: Applied Sciences (Switzerland), 2021, 11, (1), pp. 1-11
Issue Date:: 2021-01-01

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Field	Value	Language
dc.contributor.author	Jun, MY
dc.contributor.author	Karki, R
dc.contributor.author	Paudel, KR
dc.contributor.author	Panth, N
dc.contributor.author	Devkota, HP
dc.contributor.author	Kim, DW
dc.date.accessioned	2021-04-12T02:53:02Z
dc.date.available	2021-04-12T02:53:02Z
dc.date.issued	2021-01-01
dc.identifier.citation	Applied Sciences (Switzerland), 2021, 11, (1), pp. 1-11
dc.identifier.issn	1454-5101
dc.identifier.issn	2076-3417
dc.identifier.uri	http://hdl.handle.net/10453/148014
dc.description.abstract	Liensinine is a bisbenzylisoquinoline alkaloid found in various parts of the lotus (Nelumbo nucifera Gaertn.) including seeds. In this study, we explored the preventive activity of liensinine on vascular inflammation via attenuation of inflammatory mediators in macrophage and targeting the proliferation and migration of human vascular smooth muscle cells (VSMC). Anti-oxidative activity was evaluated by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay method and measuring the peroxidation of serum lipid. Inflammatory markers were studied by evaluating the release of nitric oxide (NO) and the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in macrophage cells (RAW264.7) and interleukin (IL)-6 production in VSMC. Similarly, anti-proliferative activity in VSMC was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The enzymatic activity of matrix metalloproteinase (MMP)-9 in VSMC was evaluated by gelatin zymography. Liensinine possesses significant anti-oxidative activity as revealed by the DPPH assay and inhibition of serum lipid peroxidation. Likewise, liensinine decreased NO generation in RAW 264.7 cells. In VSMC, liensinine suppressed platelet-derived growth factor stimulated proliferation and tumor necrosis factor-α (TNF-α) induced MMP-9 enzymatic activity as well as IL-6 expression. Our results revealed the potential preventive effect of liensinine on vascular inflammation, suggesting it as a promising compound for the prevention of vascular inflammation.
dc.language	English
dc.publisher	MDPI
dc.relation.ispartof	Applied Sciences (Switzerland)
dc.relation.isbasedon	10.3390/app11010386
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Liensinine prevents vascular inflammation by attenuating inflammatory mediators and modulating vsmc function
dc.type	Journal Article
utslib.citation.volume	11
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
utslib.copyright.status	open_access	*
dc.date.updated	2021-04-12T02:53:01Z
pubs.issue	1
pubs.publication-status	Published
pubs.volume	11
utslib.citation.issue	1

Abstract:

Liensinine is a bisbenzylisoquinoline alkaloid found in various parts of the lotus (Nelumbo nucifera Gaertn.) including seeds. In this study, we explored the preventive activity of liensinine on vascular inflammation via attenuation of inflammatory mediators in macrophage and targeting the proliferation and migration of human vascular smooth muscle cells (VSMC). Anti-oxidative activity was evaluated by using the 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging assay method and measuring the peroxidation of serum lipid. Inflammatory markers were studied by evaluating the release of nitric oxide (NO) and the protein levels of inducible nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in macrophage cells (RAW264.7) and interleukin (IL)-6 production in VSMC. Similarly, anti-proliferative activity in VSMC was evaluated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The enzymatic activity of matrix metalloproteinase (MMP)-9 in VSMC was evaluated by gelatin zymography. Liensinine possesses significant anti-oxidative activity as revealed by the DPPH assay and inhibition of serum lipid peroxidation. Likewise, liensinine decreased NO generation in RAW 264.7 cells. In VSMC, liensinine suppressed platelet-derived growth factor stimulated proliferation and tumor necrosis factor-α (TNF-α) induced MMP-9 enzymatic activity as well as IL-6 expression. Our results revealed the potential preventive effect of liensinine on vascular inflammation, suggesting it as a promising compound for the prevention of vascular inflammation.

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http://hdl.handle.net/10453/148014