Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal <i>Escherichia coli</i> of Multiple Sources.

Massella, E; Giacometti, F; Bonilauri, P; Reid, CJ; Djordjevic, SP; Merialdi, G; Bacci, C; Fiorentini, L; Massi, P; Bardasi, L; Rubini, S; Savini, F; Serraino, A; Piva, S

Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal <i>Escherichia coli</i> of Multiple Sources.

Massella, E Giacometti, F Bonilauri, P Reid, CJ Djordjevic, SP Merialdi, G Bacci, C Fiorentini, L Massi, P Bardasi, L Rubini, S Savini, F Serraino, A Piva, S

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Publisher:: MDPI AG
Publication Type:: Journal Article
Citation:: Antibiotics (Basel, Switzerland), 2021, 10, (4), pp. 351-351
Issue Date:: 2021-03-26

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Field	Value	Language
dc.contributor.author	Massella, E
dc.contributor.author	Giacometti, F
dc.contributor.author	Bonilauri, P
dc.contributor.author	Reid, CJ
dc.contributor.author	Djordjevic, SP
dc.contributor.author	Merialdi, G
dc.contributor.author	Bacci, C
dc.contributor.author	Fiorentini, L
dc.contributor.author	Massi, P
dc.contributor.author	Bardasi, L
dc.contributor.author	Rubini, S
dc.contributor.author	Savini, F
dc.contributor.author	Serraino, A
dc.contributor.author	Piva, S
dc.date.accessioned	2021-04-14T01:45:49Z
dc.date.available	2021-03-19
dc.date.available	2021-04-14T01:45:49Z
dc.date.issued	2021-03-26
dc.identifier.citation	Antibiotics (Basel, Switzerland), 2021, 10, (4), pp. 351-351
dc.identifier.issn	2079-6382
dc.identifier.issn	2079-6382
dc.identifier.uri	http://hdl.handle.net/10453/148079
dc.description.abstract	We recently described the genetic antimicrobial resistance and virulence profile of a collection of 279 commensal <i>E. coli</i> of food-producing animal (FPA), pet, wildlife and human origin. Phenotypic antimicrobial resistance (AMR) and the role of commensal <i>E. coli</i> as reservoir of extra-intestinal pathogenic <i>Escherichia coli</i> (ExPEC) virulence-associated genes (VAGs) or as potential ExPEC pathogens were evaluated. The most common phenotypic resistance was to tetracycline (76/279, 27.24%), sulfamethoxazole/trimethoprim (73/279, 26.16%), streptomycin and sulfisoxazole (71/279, 25.45% both) among the overall collection. Poultry and rabbit were the sources mostly associated to AMR, with a significant resistance rate (<i>p</i> > 0.01) to quinolones, streptomycin, sulphonamides, tetracycline and, only for poultry, to ampicillin and chloramphenicol. Finally, rabbit was the source mostly associated to colistin resistance. Different pandemic (ST69/69, ST95, ST131) and emerging (ST10/ST10, ST23, ST58, ST117, ST405, ST648) ExPEC sequence types (STs) were identified among the collection, especially in poultry source. Both ST groups carried high number of ExPEC VAGs (pandemic ExPEC STs, mean = 8.92; emerging ExPEC STs, mean = 6.43) and showed phenotypic resistance to different antimicrobials (pandemic ExPEC STs, mean = 2.23; emerging ExPEC STs, mean = 2.43), suggesting their role as potential ExPEC pathogens. Variable phenotypic resistance and ExPEC VAG distribution was also observed in uncommon ExPEC lineages, suggesting commensal flora as a potential reservoir of virulence (mean = 3.80) and antimicrobial resistance (mean = 1.69) determinants.
dc.format	Electronic
dc.language	eng
dc.publisher	MDPI AG
dc.relation.ispartof	Antibiotics (Basel, Switzerland)
dc.relation.isbasedon	10.3390/antibiotics10040351
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Antimicrobial Resistance Profile and ExPEC Virulence Potential in Commensal <i>Escherichia coli</i> of Multiple Sources.
dc.type	Journal Article
utslib.citation.volume	10
utslib.location.activity	Switzerland
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
dc.date.updated	2021-04-14T01:45:48Z
pubs.issue	4
pubs.publication-status	Published
pubs.volume	10
utslib.citation.issue	4

Abstract:

We recently described the genetic antimicrobial resistance and virulence profile of a collection of 279 commensal E. coli of food-producing animal (FPA), pet, wildlife and human origin. Phenotypic antimicrobial resistance (AMR) and the role of commensal E. coli as reservoir of extra-intestinal pathogenic Escherichia coli (ExPEC) virulence-associated genes (VAGs) or as potential ExPEC pathogens were evaluated. The most common phenotypic resistance was to tetracycline (76/279, 27.24%), sulfamethoxazole/trimethoprim (73/279, 26.16%), streptomycin and sulfisoxazole (71/279, 25.45% both) among the overall collection. Poultry and rabbit were the sources mostly associated to AMR, with a significant resistance rate (p > 0.01) to quinolones, streptomycin, sulphonamides, tetracycline and, only for poultry, to ampicillin and chloramphenicol. Finally, rabbit was the source mostly associated to colistin resistance. Different pandemic (ST69/69*, ST95, ST131) and emerging (ST10/ST10*, ST23, ST58, ST117, ST405, ST648) ExPEC sequence types (STs) were identified among the collection, especially in poultry source. Both ST groups carried high number of ExPEC VAGs (pandemic ExPEC STs, mean = 8.92; emerging ExPEC STs, mean = 6.43) and showed phenotypic resistance to different antimicrobials (pandemic ExPEC STs, mean = 2.23; emerging ExPEC STs, mean = 2.43), suggesting their role as potential ExPEC pathogens. Variable phenotypic resistance and ExPEC VAG distribution was also observed in uncommon ExPEC lineages, suggesting commensal flora as a potential reservoir of virulence (mean = 3.80) and antimicrobial resistance (mean = 1.69) determinants.

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http://hdl.handle.net/10453/148079