Dissemination of novel Tn7 family transposons carrying genes for synthesis and uptake of fimsbactin siderophores among Acinetobacter baumannii isolates.

Hamidian, M; Hall, RM

Dissemination of novel Tn7 family transposons carrying genes for synthesis and uptake of fimsbactin siderophores among Acinetobacter baumannii isolates.

Hamidian, M

Hall, RM

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Publisher:: Microbiology Society
Publication Type:: Journal Article
Citation:: Microbial genomics, 2021, 7, (3)
Issue Date:: 2021-03-22

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Field	Value	Language
dc.contributor.author	Hamidian, M https://orcid.org/0000-0002-3614-7261
dc.contributor.author	Hall, RM
dc.date.accessioned	2021-04-15T05:10:08Z
dc.date.available	2021-04-15T05:10:08Z
dc.date.issued	2021-03-22
dc.identifier.citation	Microbial genomics, 2021, 7, (3)
dc.identifier.issn	2057-5858
dc.identifier.issn	2057-5858
dc.identifier.uri	http://hdl.handle.net/10453/148123
dc.description.abstract	<i>Acinetobacter baumannii</i> is a successful opportunistic pathogen that can compete for iron under iron-limiting conditions. Here, large novel transposons that carry genes for synthesis and transport of the fimsbactin siderophores present in some <i>A. baumannii</i> strains were examined. Tn<i>6171</i>, originally found in the <i>A. baumannii</i> global clone 1 (GC1) lineage 2 isolate D36, includes <i>tns</i> genes encoding proteins related to the TnsA, TnsB, TnsC transposition proteins (50-59 % identity), TnsD targeting protein (43 % identity) and TnsE (31 % identity) of Tn<i>7</i>, and is found in the chromosome downstream of the <i>glmS</i> gene, the preferred location for Tn<i>7</i>, flanked by a 5 bp target site duplication. Tn<i>6171</i> is bounded by 29 bp inverted repeats and, like Tn<i>7</i>, includes additional TnsB binding sites at each end. Tn<i>6171</i> or minor variants were detected in the equivalent location in complete or draft genomes of several further <i>A. baumannii</i> isolates belonging to GC1 [sequence type (ST) 1, ST81, ST94, ST328, ST623, ST717], GC2 (ST2) and ST10. However, in some of these isolates the surrounding <i>glmS</i> region was clearly derived from a different <i>A. baumannii</i> lineage, indicating that the transposon may have been acquired by replacement of a segment of the chromosome. A recombination-free phylogeny revealed that there were several transposon acquisition events in GC1. The GC1 isolates were mainly lineage 2, but a potential third lineage was also detected. A related transposon, designated Tn<i>6552</i>, was detected in ATCC 17978 (ST437) and other ST437 isolates. However, the Tn<i>6552 tnsD</i> targeting gene was interrupted by an ISAba12, and Tn<i>6552</i> is not downstream of <i>glmS</i>.
dc.format	Print-Electronic
dc.language	eng
dc.publisher	Microbiology Society
dc.relation	http://purl.org/au-research/grants/arc/DE200100111
dc.relation.ispartof	Microbial genomics
dc.relation.isbasedon	10.1099/mgen.0.000548
dc.rights	info:eu-repo/semantics/openAccess
dc.subject	0604 Genetics, 0605 Microbiology
dc.title	Dissemination of novel Tn<i>7</i> family transposons carrying genes for synthesis and uptake of fimsbactin siderophores among <i>Acinetobacter baumannii</i> isolates.
dc.type	Journal Article
utslib.citation.volume	7
utslib.location.activity	England
utslib.for	0604 Genetics
utslib.for	0605 Microbiology
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - ithree - Institute of Infection, Immunity and Innovation
utslib.copyright.status	open_access	*
dc.date.updated	2021-04-15T05:10:07Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	7
utslib.citation.issue	3

Abstract:

Acinetobacter baumannii is a successful opportunistic pathogen that can compete for iron under iron-limiting conditions. Here, large novel transposons that carry genes for synthesis and transport of the fimsbactin siderophores present in some A. baumannii strains were examined. Tn6171, originally found in the A. baumannii global clone 1 (GC1) lineage 2 isolate D36, includes tns genes encoding proteins related to the TnsA, TnsB, TnsC transposition proteins (50-59 % identity), TnsD targeting protein (43 % identity) and TnsE (31 % identity) of Tn7, and is found in the chromosome downstream of the glmS gene, the preferred location for Tn7, flanked by a 5 bp target site duplication. Tn6171 is bounded by 29 bp inverted repeats and, like Tn7, includes additional TnsB binding sites at each end. Tn6171 or minor variants were detected in the equivalent location in complete or draft genomes of several further A. baumannii isolates belonging to GC1 [sequence type (ST) 1, ST81, ST94, ST328, ST623, ST717], GC2 (ST2) and ST10. However, in some of these isolates the surrounding glmS region was clearly derived from a different A. baumannii lineage, indicating that the transposon may have been acquired by replacement of a segment of the chromosome. A recombination-free phylogeny revealed that there were several transposon acquisition events in GC1. The GC1 isolates were mainly lineage 2, but a potential third lineage was also detected. A related transposon, designated Tn6552, was detected in ATCC 17978 (ST437) and other ST437 isolates. However, the Tn6552 tnsD targeting gene was interrupted by an ISAba12, and Tn6552 is not downstream of glmS.

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http://hdl.handle.net/10453/148123

Dissemination of novel Tn<i>7</i> family transposons carrying genes for synthesis and uptake of fimsbactin siderophores among <i>Acinetobacter baumannii</i> isolates.