Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro

Thomas, M; Lawrence, A; Kroon, S; Vodstrcil, LA; Phillips, S; Hocking, JS; Timms, P; Huston, WM

Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro

Thomas, M Lawrence, A Kroon, S Vodstrcil, LA Phillips, S Hocking, JS Timms, P Huston, WM

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Publisher:: Microbiology Society
Publication Type:: Journal Article
Citation:: Access Microbiology, 2021, 3, (3)
Issue Date:: 2021-03-01

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Field	Value	Language
dc.contributor.author	Thomas, M
dc.contributor.author	Lawrence, A
dc.contributor.author	Kroon, S
dc.contributor.author	Vodstrcil, LA
dc.contributor.author	Phillips, S
dc.contributor.author	Hocking, JS
dc.contributor.author	Timms, P
dc.contributor.author	Huston, WM
dc.date.accessioned	2021-04-17T01:30:21Z
dc.date.available	2021-04-17T01:30:21Z
dc.date.issued	2021-03-01
dc.identifier.citation	Access Microbiology, 2021, 3, (3)
dc.identifier.issn	2516-8290
dc.identifier.issn	2516-8290
dc.identifier.uri	http://hdl.handle.net/10453/148181
dc.description.abstract	<jats:p>Urogenital <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.7767" xlink:type="simple">Chlamydia trachomatis</jats:ext-link> </jats:named-content> </jats:italic> infection is the most common sexually transmitted bacterial infection throughout the world. While progress has been made to better understand how type strains develop and respond to environmental stress <jats:italic>in vitro</jats:italic>, very few studies have examined how clinical isolates behave under similar conditions. Here, we examined the development and persistence phenotypes of several clinical isolates, to determine how similar they are to each other, and the type strain <jats:italic> <jats:named-content content-type="species"> <jats:ext-link xmlns:xlink="http://www.w3.org/1999/xlink" ext-link-type="uri" xlink:href="http://doi.org/10.1601/nm.7767" xlink:type="simple">C. trachomatis</jats:ext-link> </jats:named-content> </jats:italic> D/UW-3/Cx. The type strain was shown to produce infectious progeny at a higher magnitude than each of the clinical isolates, in each of the six tested cell lines. All chlamydial strains produced the highest number of infectious progeny at 44 h post-infection in the McCoy B murine fibroblast cell line, yet showed higher levels of infectivity in the MCF-7 human epithelial cell line. The clinical isolates were shown to be more susceptible than the type strain to the effects of penicillin and iron deprivation persistence models in the MCF-7 cell line. While subtle differences between clinical isolates were observed throughout the experiments conducted, no significant differences were identified. This study reinforces the importance of examining clinical isolates when trying to relate <jats:italic>in vitro</jats:italic> data to clinical outcomes, as well as the importance of considering the adaptations many type strains have to being cultured <jats:italic>in vitro</jats:italic>.</jats:p>
dc.language	en
dc.publisher	Microbiology Society
dc.relation.ispartof	Access Microbiology
dc.relation.isbasedon	10.1099/acmi.0.000204
dc.rights	info:eu-repo/semantics/openAccess
dc.title	Chlamydial clinical isolates show subtle differences in persistence phenotypes and growth in vitro
dc.type	Journal Article
utslib.citation.volume	3
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Faculty of Science/School of Life Sciences
utslib.copyright.status	open_access	*
dc.date.updated	2021-04-17T01:30:18Z
pubs.issue	3
pubs.publication-status	Published
pubs.volume	3
utslib.citation.issue	3

Abstract:

Urogenital

Chlamydia trachomatis

infection is the most common sexually transmitted bacterial infection throughout the world. While progress has been made to better understand how type strains develop and respond to environmental stress in vitro, very few studies have examined how clinical isolates behave under similar conditions. Here, we examined the development and persistence phenotypes of several clinical isolates, to determine how similar they are to each other, and the type strain

C. trachomatis

D/UW-3/Cx. The type strain was shown to produce infectious progeny at a higher magnitude than each of the clinical isolates, in each of the six tested cell lines. All chlamydial strains produced the highest number of infectious progeny at 44 h post-infection in the McCoy B murine fibroblast cell line, yet showed higher levels of infectivity in the MCF-7 human epithelial cell line. The clinical isolates were shown to be more susceptible than the type strain to the effects of penicillin and iron deprivation persistence models in the MCF-7 cell line. While subtle differences between clinical isolates were observed throughout the experiments conducted, no significant differences were identified. This study reinforces the importance of examining clinical isolates when trying to relate in vitro data to clinical outcomes, as well as the importance of considering the adaptations many type strains have to being cultured in vitro.

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http://hdl.handle.net/10453/148181