Aerotaxis in Desulfovibrio

Eschemann, A; Kühl, M; Cypionka, H

Aerotaxis in Desulfovibrio

Eschemann, A Kühl, M

Cypionka, H

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Publication Type:: Journal Article
Citation:: Environmental Microbiology, 1999, 1 (6), pp. 489 - 494
Issue Date:: 1999-01-01

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Field	Value	Language
dc.contributor.author	Eschemann, A	en_US
dc.contributor.author	Kühl, M https://orcid.org/0000-0002-1792-4790	en_US
dc.contributor.author	Cypionka, H	en_US
dc.date.issued	1999-01-01	en_US
dc.identifier.citation	Environmental Microbiology, 1999, 1 (6), pp. 489 - 494	en_US
dc.identifier.issn	1462-2912	en_US
dc.identifier.uri	http://hdl.handle.net/10453/14823
dc.description.abstract	Aerotaxis of two sulphate-reducing bacteria, the freshwater strain Desulfovibrio desulfuricans CSN (DSM 9104) and the marine strain Desulfovibrio oxyclinae N13 (DSM 11498), was studied using capillary microslides, microscopy and oxygen microsensors. The bacteria formed ring-shaped bands in oxygen diffusion gradients surrounding O2 bubbles, which were placed into anoxic sulphate-free cell suspensions in capillary microslides. The radial expansion of the oxic volume by diffusion was stopped by aerobic respiration. Bands were formed by cells avoiding high O2 levels near the O2 bubble, as well as by cells entering from the surrounding anoxic zone. At the inner edge of the bands, O2 levels of up to 20% air saturation (50μM O2) were found, while the outer edge always coincided with the oxic-anoxic interface. Ring diameters and O2 concentrations at the inner edge of the band depended on the cell density and the strain used in the suspension. Band formation did not occur in the absence of an electron donor (S mM lactate) or when N2 gas bubbles were used. Both strains were highly motile with velocities of ≈ 32 μm s-1 during forward runs, and 7 μm s-1 during backward runs respectively. Within the bands, cells moved in circles of about 20 μm diameter, while cells outside the band exhibited straighter or only slightly bent traces. It is concluded that the capacity of respiration at high rates and the positive and negative aerotactical responses of Desulfovibrio provide an efficient strategy for removing O2 from the habitat in situations where sufficient electron donors and high cell densities are present.	en_US
dc.relation.ispartof	Environmental Microbiology	en_US
dc.relation.isbasedon	10.1046/j.1462-2920.1999.00057.x	en_US
dc.subject.classification	Microbiology	en_US
dc.subject.mesh	Desulfovibrio	en_US
dc.subject.mesh	Oxygen	en_US
dc.subject.mesh	Water Microbiology	en_US
dc.subject.mesh	Chemotaxis	en_US
dc.subject.mesh	Oxygen Consumption	en_US
dc.title	Aerotaxis in Desulfovibrio	en_US
dc.type	Journal Article
utslib.citation.volume	6	en_US
utslib.citation.volume	1	en_US
utslib.for	0605 Microbiology	en_US
utslib.for	0603 Evolutionary Biology	en_US
pubs.embargo.period	Not known	en_US
pubs.organisational-group	/University of Technology Sydney
pubs.organisational-group	/University of Technology Sydney/Faculty of Science
pubs.organisational-group	/University of Technology Sydney/Strength - C3 - Climate Change Cluster
utslib.copyright.status	closed_access
pubs.issue	6	en_US
pubs.publication-status	Published	en_US
pubs.volume	1	en_US

Abstract:

Aerotaxis of two sulphate-reducing bacteria, the freshwater strain Desulfovibrio desulfuricans CSN (DSM 9104) and the marine strain Desulfovibrio oxyclinae N13 (DSM 11498), was studied using capillary microslides, microscopy and oxygen microsensors. The bacteria formed ring-shaped bands in oxygen diffusion gradients surrounding O2 bubbles, which were placed into anoxic sulphate-free cell suspensions in capillary microslides. The radial expansion of the oxic volume by diffusion was stopped by aerobic respiration. Bands were formed by cells avoiding high O2 levels near the O2 bubble, as well as by cells entering from the surrounding anoxic zone. At the inner edge of the bands, O2 levels of up to 20% air saturation (50μM O2) were found, while the outer edge always coincided with the oxic-anoxic interface. Ring diameters and O2 concentrations at the inner edge of the band depended on the cell density and the strain used in the suspension. Band formation did not occur in the absence of an electron donor (S mM lactate) or when N2 gas bubbles were used. Both strains were highly motile with velocities of ≈ 32 μm s-1 during forward runs, and 7 μm s-1 during backward runs respectively. Within the bands, cells moved in circles of about 20 μm diameter, while cells outside the band exhibited straighter or only slightly bent traces. It is concluded that the capacity of respiration at high rates and the positive and negative aerotactical responses of Desulfovibrio provide an efficient strategy for removing O2 from the habitat in situations where sufficient electron donors and high cell densities are present.

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http://hdl.handle.net/10453/14823