Termination structures in the Escherichia coli chromosome replication fork trap

Publisher:
Academic Press
Publication Type:
Journal Article
Citation:
Journal of Molecular Biology, 2009, 387 (3), pp. 532 - 539
Issue Date:
2009-01
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The Escherichia coli chromosome contains two opposed sets of unidirectional DNA replication pause (Ter) sites that, according to the replication fork trap theory, control the termination of chromosome replication by restricting replication fork fusion to the terminus region. In contrast, a recent hypothesis suggested that termination occurs at the dif locus instead. Using twodimensional agarose gel electrophoresis, we examined DNA replication intermediates at the Ter sites and at dif in wild-type cells. Two definitive signatures of site-specific terminationspecific replication fork arrest and converging replication forkswere clearly detected at Ter sites, but not at dif. We also detected a significant pause during the latter stages of replication fork convergence at Ter sites. Quantification of fork pausing at the Ter sites in both their native chromosomal context and the plasmid context further supported the fork trap model.
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